RegulonDB RegulonDB 10.8: Operon Form
   

cusCFBA operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: cusCFBA
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: cusCFBA
Synonym(s): ylcBCD-ybdE
Gene(s): cusC, cusF, cusB, cusA   Genome Browser M3D Gene expression COLOMBOS
Note(s): cusCFBA expression is induced under Zn excess in a CusR-dependent way Xu Z,2019.
This transcription unit may even include the pheP gene that is downstream of the cusA gene.
cusB expression is increased at least 5-fold by indole Hirakawa H,2005.
The mRNA that contains the cusA gene has been observed mainly in the cellular membrane 31540875.
Evidence: [LTED] Length of transcript experimentally determined
Reference(s): [1] Franke S., et al., 2001
Promoter
Name: cusCp
+1: 595573
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 27
Sequence: tgacaattttgtcatttttctgtcaccggaaaatcagagcctggcgagtaaagttggcggCataaaatcaccagaaattat
                      -35                        -10        +1                   
Evidence: [HIPP]
[TIM]
Reference(s): [1] Franke S., et al., 2001
[2] Yamamoto K., et al., 2005
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal CusR-Pasp activator cusCp 595512 595528 -53.5 tattggcaaaATGACAATTTTGTCATTtttctgtcac nd [APIORCISFBSCS], [BPP], [CV(GEA)], [CV(GEA)], [GEA] [2], [4], [5], [6], [7]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal HprR-P activator cusCp 595511 595529 -53.5 ttattggcaaAATGACAATTTTGTCATTTttctgtcacc nd [APIORCISFBSCS], [BPP] [5], [6]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal PhoB-Phosphorylated activator cusCp 595510 595528 -54.0 cttattggcaAAATGACAATTTTGTCATTtttctgtcac nd [APIORCISFBSCS], [BPP], [CV(GEA)], [CV(GEA)], [GEA] [3]


RNA cis-regulatory element    
Regulation, transcriptional elongation  
Attenuator type: Transcriptional
Strand: forward
  Structure type Energy LeftPos RightPos Sequence (RNA-strand)
  terminator -7.3 595505 595533 atcgcttattGGCAAAATGACAATTTTGTCATTTTTCTgtcaccggaa
  anti-terminator -5.2 595472 595507 cgggcaattcTAGAGTAGCGGGATCAGATGGCAATCGCTTATTGGcaaaatgaca
  anti-anti-terminator -9.6 595450 595484 catatttcctCCGCATGTTGCCCGGGCAATTCTAGAGTAGCGGGatcagatggc
Notes: "The provided "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appear as the reverse complement of the sequence delimited by LeftPos-RigtPos"





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