RegulonDB RegulonDB 10.10: Operon Form
   

cusRS operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: cusRS
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: cusRS
Synonym(s): ylcA-ybcZ
Gene(s): cusS, cusR   Genome Browser M3D Gene expression COLOMBOS
Evidence: [LTED] Length of transcript experimentally determined
Reference(s): [1] Franke S., et al., 2001
Promoter
Name: cusRp
+1: 595462
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 19
Sequence: aaaattgtcattttgccaataagcgattgccatctgatcccgctactctagaattgcccgGgcaacatgcggaggaaatat
                           -35                   -10        +1                   
Evidence: [HIPP]
[ICWHO]
[TIM]
Reference(s): [1] Franke S., et al., 2001
[2] Huerta AM., et al., 2003
[3] Yamamoto K., et al., 2005
TF binding sites (TFBSs)
[CusR,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal CusR-phosphorylated activator cusRp 595512 595528 -57.5 gtgacagaaaAATGACAAAATTGTCATtttgccaata nd [GEA], [APIORCISFBSCS], [BPP] [3], [4], [5]
[HprR,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal HprR-phosphorylated activator cusRp 595511 595529 -57.5 ggtgacagaaAAATGACAAAATTGTCATTttgccaataa nd [APIORCISFBSCS], [BPP] [6]
[PhoB,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal PhoB-phosphorylated activator cusRp 595510 595528 -57.0 gtgacagaaaAATGACAAAATTGTCATTTtgccaataag nd [GEA], [APIORCISFBSCS], [BPP] [7]


Evidence    

 [HIPP] Human inference of promoter position

 [ICWHO] Inferred computationally without human oversight

 [TIM] Transcription initiation mapping

 [GEA] Gene expression analysis

 [APIORCISFBSCS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.

 [BPP] Binding of purified proteins


Reference(s)    

 [1] Franke S., Grass G., Nies DH., 2001, The product of the ybdE gene of the Escherichia coli chromosome is involved in detoxification of silver ions., Microbiology 147(Pt 4):965-72

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [3] Yamamoto K., Ishihama A., 2005, Transcriptional response of Escherichia coli to external copper., Mol Microbiol 56(1):215-27

 [4] Munson GP., Lam DL., Outten FW., O'Halloran TV., 2000, Identification of a copper-responsive two-component system on the chromosome of Escherichia coli K-12., J Bacteriol 182(20):5864-71

 [5] Xu Z., Wang P., Wang H., Yu ZH., Au-Yeung HY., Hirayama T., Sun H., Yan A., 2019, Zinc excess increases cellular demand for iron and decreases tolerance to copper in Escherichia coli., J Biol Chem 294(45):16978-16991

 [6] Urano H., Umezawa Y., Yamamoto K., Ishihama A., Ogasawara H., 2015, Cooperative regulation of the common target genes between H2O2-sensing YedVW and Cu2+-sensing CusSR in Escherichia coli., Microbiology 161(Pt 4):729-38

 [7] Yang C., Huang TW., Wen SY., Chang CY., Tsai SF., Wu WF., Chang CH., 2012, Genome-wide PhoB binding and gene expression profiles reveal the hierarchical gene regulatory network of phosphate starvation in Escherichia coli., PLoS One 7(10):e47314

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