RegulonDB RegulonDB 10.9: Operon Form
   

glcDEFGBA operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: glcDEFGBA
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: glcDEFGBA
Gene(s): glcA, glcB, glcG, glcF, glcE, glcD   Genome Browser M3D Gene expression COLOMBOS
Evidence: [LTED] Length of transcript experimentally determined
Reference(s): [1] Nunez MF., et al., 2001
Promoter
Name: glcDp
+1: 3128076
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 55
Sequence: ggtctgaccggtagctaaagagatagacgaaaacgaaaagcccgcttaataactgttcacAgaagcagcgcgcaaaaatca
                        -35                    -10          +1                   
Evidence: [HIPP]
[ICWHO]
[TIM]
Reference(s): [2] Huerta AM., et al., 2003
[3] Pellicer MT., et al., 1999
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote ArcA-Phosphorylated repressor glcDp 3128173 3128187 -104.0 ttatgttacaTCAATTTAACATTGAGttaaccaaga nd [AIBSCS], [AIBSPD], [BPP], [CCE], [GEA] [3], [4]
remote ArcA-Phosphorylated repressor glcDp 3128180 3128194 -111.0 taagtgattaTGTTACATCAATTTAAcattgagtta nd [AIBSPD], [BPP], [CCE], [GEA] [3]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote GlcC-glycolate activator glcDp 3128245 3128259 -176.0 cccggacctcGTGCACAGGTAGGACCaatttttctg nd [BCE], [GEA], [SM] [3]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote IHF activator glcDp 3128177 3128189 -107.0 gattatgttaCATCAATTTAACATtgagttaacc nd [BCE], [GEA] [3]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal PdhR repressor glcDp 3128124 3128140 -56.0 agctggaaaaAAAATGGTCTGACCGGTAgctaaagaga nd [AIBSCS], [BPP], [GEA] [5]


Transcription unit       
Name: glcBA
Gene(s): glcA, glcB   Genome Browser M3D Gene expression COLOMBOS
Evidence: [PAGTSBP] Products of adjacent genes in the same biological process
Promoter
Name: glcBp
+1: Unknown
Note(s): Zaslaver et al. demonstrated in 2006, by means of a library of fluorescent transcription fusions, that this promoter can be transcribed in vitro Zaslaver A,2006. Based on this, a putative promoter was suggested, but the +1 site of the transcription initiation has not been determined, although there exists promoter activity.
Evidence: [IEP]
Reference(s): [6] Zaslaver A., et al., 2006


RNA cis-regulatory element    
Regulation, transcriptional elongation  
Attenuator type: Transcriptional
Strand: reverse
  Structure type Energy LeftPos RightPos Sequence (RNA-strand)
  terminator -11.3 3121354 3121398 ggtagcaattGCGTTAGCGGCGTACTGTTGCTGTAAAGTATAACGCCTATATTTtacagttcag
  anti-terminator -7.72 3121385 3121417 atatacttgtTATGCCAGAGGTAGCAATTGCGTTAGCGGCGTactgttgctg
  anti-anti-terminator -13.51 3121404 3121463 taacgatactCTGCGTCTGCGTGTGCGGAACAGAAACCCTTACCCGATATACTTGTTATGCCAGAGGTAgcaattgcgt
Notes: "The provided "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appear as the reverse complement of the sequence delimited by LeftPos-RigtPos"





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