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| Operon |  |
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| Name: | serA |
| This page displays every known transcription unit of this operon and their known regulation. | |
| Name: | serA | |||||||||
| Synonym(s): | OP00315 | |||||||||
| Gene(s): | serA Genome Browser M3D Gene expression COLOMBOS | |||||||||
| Note(s): | The serA gene codes for 3-phosphoglycerate dehydrogenase, which catalyzes the first step in the biosynthesis of serine from glucose Tobey KL,1986. It was observed that the serA gene is repressed by the Nac protein and that there are two Nac-binding sites overlapping the serAp1 promoter Blauwkamp TA,2002 Therefore, we linked this regulation to the serAp1 promoter; however, the serAp2 promoter could be also affected by Nac. Blauwkamp TA,2002 et al. hypothesized that Nac represses serine biosynthesis, through the repression of serA, as part of a cellular mechanism to reduce metabolism when cells become starved for nitrogen. On the other hand, there is indirect evidence of Lrp regulating serA transcription (gene expression analysis) Tuan LR,1990 but no further studies have been done to prove a direct interaction. Based on genome-wide analysis, 472 single-gene knockouts were studied to determine their anaerobic fermentation products, based on the control of redox reactions. It was determined that the combined knockout of the guaB, pyrD, serA, fnr, arcA, and arcB genes enhanced D-lactate overproduction Kim HJ,2013 |
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| Evidence: | [BTEI] Boundaries of transcription experimentally identified | |||||||||
| Reference(s): |
[1] Calvo JM., et al., 1994 [2] Tobey KL., et al., 1986 [3] Tuan LR., et al., 1990 |
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| Promoter | ||||||||||
| Name: | serAp1 | |||||||||
| +1: | 3058456 | |||||||||
| Sigma Factor: | Sigma70 Sigmulon | |||||||||
| Distance from start of the gene: | 46 | |||||||||
| Sequence: |
tcaacatcgcgacgcaaacgttcatattgccgcaatattattttttgatatgttgaaaggCggatgcaaatccgcacacaa -35 -10 +1 |
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| Evidence: |
[HIPP] [ICWHO] [IHBCE] [IMP] [TIM] |
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| Reference(s): |
[4] Huerta AM., et al., 2003 [5] Yang L., et al., 2002 |
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| Type | Transcription factor | Function | Promoter | Binding Sites | Growth Conditions | Evidence (Confirmed, Strong, Weak) | Reference(s) | |||
|---|---|---|---|---|---|---|---|---|---|---|
| LeftPos | RightPos | Central Rel-Pos | Sequence | |||||||
| proximal | Nac | repressor | serAp1 | 3058468 | 3058482 | -19.0 | tattgccgcaATATTATTTTTTGATatgttgaaag | nd | [GEA], [RSE], [AIBSPD], [APIORCISFBSCS], [CSE] | [6], [7] |
| proximal | Nac | repressor | serAp1 | 3058479 | 3058493 | -30.0 | gcaaacgttcATATTGCCGCAATATtattttttga | nd | [GEA], [APIORCISFBSCS] | [6] |
| Name: | serA | |||||||||
| Gene(s): | serA Genome Browser M3D Gene expression COLOMBOS | |||||||||
| Note(s): | The serA gene codes for 3-phosphoglycerate dehydrogenase, which catalyzes the first step in the biosynthesis of serine from glucose Tobey KL,1986. It was observed that the serA gene is repressed by the Nac protein and that there are two Nac-binding sites overlapping the serAp1 promoter Blauwkamp TA,2002 Therefore, we linked this regulation to the serAp1 promoter; however, the serAp2 promoter could be also affected by Nac. Blauwkamp TA,2002 et al. hypothesized that Nac represses serine biosynthesis, through the repression of serA, as part of a cellular mechanism to reduce metabolism when cells become starved for nitrogen. On the other hand, there is indirect evidence of Lrp regulating serA transcription (gene expression analysis) Tuan LR,1990 but no further studies have been done to prove a direct interaction | |||||||||
| Evidence: | [BTEI] Boundaries of transcription experimentally identified | |||||||||
| Reference(s): | [2] Tobey KL., et al., 1986 | |||||||||
| Promoter | ||||||||||
| Name: | serAp2 | |||||||||
| +1: | 3058549 | |||||||||
| Sigma Factor: | Sigma70 Sigmulon | |||||||||
| Distance from start of the gene: | 139 | |||||||||
| Sequence: |
gtgtcacgcttttaccaggcaattgtcgattgctctaaataaatcctctaaaccagcataTtcatccaagaattacctttg -35 -10 +1 |
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| Evidence: |
[HIPP] [ICWHO] [IHBCE] [IMP] [TIM] |
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| Reference(s): |
[4] Huerta AM., et al., 2003 [5] Yang L., et al., 2002 |
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| Type | Transcription factor | Function | Promoter | Binding Sites | Growth Conditions | Evidence (Confirmed, Strong, Weak) | Reference(s) | |||
|---|---|---|---|---|---|---|---|---|---|---|
| LeftPos | RightPos | Central Rel-Pos | Sequence | |||||||
| proximal | CRP-cyclic-AMP | activator | serAp2 | 3058618 | 3058639 | -80.0 | cccccgttaaAAAAATTCTCTTCATTAAATTTggtgacatgt | nd | [GEA], [APIORCISFBSCS] | [5] |
| Reference(s) |
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|---|---|
[HIPP] Human inference of promoter position