RegulonDB RegulonDB 10.8: Operon Form
   

serA operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: serA
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: serA
Synonym(s): OP00315
Gene(s): serA   Genome Browser M3D Gene expression COLOMBOS
Note(s): The serA gene codes for 3-phosphoglycerate dehydrogenase, which catalyzes the first step in the biosynthesis of serine from glucose Tobey KL,1986. It was observed that the serA gene is repressed by the Nac protein and that there are two Nac-binding sites overlapping the serAp1 promoter Blauwkamp TA,2002 Therefore, we linked this regulation to the serAp1 promoter; however, the serAp2 promoter could be also affected by Nac. Blauwkamp TA,2002 et al. hypothesized that Nac represses serine biosynthesis, through the repression of serA, as part of a cellular mechanism to reduce metabolism when cells become starved for nitrogen. On the other hand, there is indirect evidence of Lrp regulating serA transcription (gene expression analysis) Tuan LR,1990 but no further studies have been done to prove a direct interaction.
Based on genome-wide analysis, 472 single-gene knockouts were studied to determine their anaerobic fermentation products, based on the control of redox reactions. It was determined that the combined knockout of the guaB, pyrD, serA, fnr, arcA, and arcB genes enhanced D-lactate overproduction Kim HJ,2013
Evidence: [BTEI] Boundaries of transcription experimentally identified
Reference(s): [1] Calvo JM., et al., 1994
[2] Tobey KL., et al., 1986
[3] Tuan LR., et al., 1990
Promoter
Name: serAp1
+1: 3058456
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 46
Sequence: tcaacatcgcgacgcaaacgttcatattgccgcaatattattttttgatatgttgaaaggCggatgcaaatccgcacacaa
                           -35                   -10        +1                   
Evidence: [HIPP]
[IMP]
[TIM]
Reference(s): [4] Yang L., et al., 2002
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd Lrp activator serAp1 nd nd nd nd nd [BPP], [GEA] [4]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal Nac repressor serAp1 3058468 3058482 -19.0 tattgccgcaATATTATTTTTTGATatgttgaaag nd [APIORCISFBSCS], [CV(GEA/ROMA)], [GEA] [5]
proximal Nac repressor serAp1 3058479 3058493 -30.0 gcaaacgttcATATTGCCGCAATATtattttttga nd [APIORCISFBSCS], [CV(GEA/ROMA)], [GEA] [5]


Transcription unit          
Name: serA
Gene(s): serA   Genome Browser M3D Gene expression COLOMBOS
Note(s): The serA gene codes for 3-phosphoglycerate dehydrogenase, which catalyzes the first step in the biosynthesis of serine from glucose Tobey KL,1986. It was observed that the serA gene is repressed by the Nac protein and that there are two Nac-binding sites overlapping the serAp1 promoter Blauwkamp TA,2002 Therefore, we linked this regulation to the serAp1 promoter; however, the serAp2 promoter could be also affected by Nac. Blauwkamp TA,2002 et al. hypothesized that Nac represses serine biosynthesis, through the repression of serA, as part of a cellular mechanism to reduce metabolism when cells become starved for nitrogen. On the other hand, there is indirect evidence of Lrp regulating serA transcription (gene expression analysis) Tuan LR,1990 but no further studies have been done to prove a direct interaction
Evidence: [BTEI] Boundaries of transcription experimentally identified
Reference(s): [2] Tobey KL., et al., 1986
Promoter
Name: serAp2
+1: 3058549
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 139
Sequence: gtgtcacgcttttaccaggcaattgtcgattgctctaaataaatcctctaaaccagcataTtcatccaagaattacctttg
                              -35                     -10   +1                   
Evidence: [HIPP]
[IMP]
[TIM]
Reference(s): [4] Yang L., et al., 2002
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal CRP-cAMP activator serAp2 3058618 3058639 -80.0 cccccgttaaAAAAATTCTCTTCATTAAATTTggtgacatgt nd [APIORCISFBSCS], [CV(GEA/ROMA)], [GEA] [4]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd Lrp-leucine repressor serAp2 nd nd nd nd nd [BPP], [GEA] [4]


Regulation by sRNA    
  Small RNA name (Regulator) Regulation type Mechanism Function Binding Sites Evidence Reference
LeftPos RightPos Sequence (RNA-strand)
  gcvB unknown unknown activator       [IMP] [6]
Notes: "The provided sequence is that of the RNA strand,i.e. 'U's are showed instead the 'T'"





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