RegulonDB RegulonDB 10.8: Operon Form
   

amiA-hemF operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: amiA-hemF
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: amiA-hemF
Gene(s): amiA, hemF   Genome Browser M3D Gene expression COLOMBOS
Evidence: [LTED] Length of transcript experimentally determined
Reference(s): [1] Troup B., et al., 1994
Promoter
Name: amiAp
+1: 2552241
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 111
Sequence: gtgaacggtcgaattagccaatatctgacgaaaatcggttgaaaaagtggcataatggggAgttgtcaactattgaaatga
                         -35                       -10      +1                   
Evidence: [HIPP]
[TIM]
Reference(s): [1] Troup B., et al., 1994
TF binding sites (TFBSs)
[IHF,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal IHF1 repressor amiAp 2552254 2552266 20.5 tgtcaactatTGAAATGAAAAGTaaaacaattc nd [APIORCISFBSCS] [1]
Note(s): 1The regulatory effect of IHF on the promoter exbBp is as of yet not known. However, we assigned a negative effect to this regulatory interaction based on the fact that the protein's binding site is downstream of the promoter (+21.5).1The regulatory effect of IHF on the promoter exbBp is as of yet not known. However, we assigned a negative effect to this regulatory interaction based on the fact that the protein's binding site is downstream of the promoter (+21.5).


Transcription unit          
Name: hemF
Gene(s): hemF   Genome Browser M3D Gene expression COLOMBOS
Evidence: [LTED] Length of transcript experimentally determined
Reference(s): [1] Troup B., et al., 1994
Promoter
Name: hemFp
+1: 2553205
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 20
Sequence: ccacagcgattgctgaaggcgtgatcagttatttccactggttcgacaaccagaaagcacAttcgaaaaagcgataagtta
                             -35                   -10      +1                   
Evidence: [TIM]
Reference(s): [1] Troup B., et al., 1994
TF binding sites (TFBSs)
[IHF,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal IHF1 repressor hemFp 2553206 2553218 8.0 agaaagcacaTTCGAAAAAGCGAtaagttatga nd [APIORCISFBSCS] [1]
Note(s): 1The regulatory effect of IHF on the promoter hemFp is not yet known. However, we assigned a negative effect to this regulatory interaction based on the fact that the protein's binding site overlaps with the promoter.1The regulatory effect of IHF on the promoter hemFp is not yet known. However, we assigned a negative effect to this regulatory interaction based on the fact that the protein's binding site overlaps with the promoter.


Evidence    

 [HIPP] Human inference of promoter position

 [TIM] Transcription initiation mapping

 [APIORCISFBSCS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.


Reference(s)    

 [1] Troup B., Jahn M., Hungerer C., Jahn D., 1994, Isolation of the hemF operon containing the gene for the Escherichia coli aerobic coproporphyrinogen III oxidase by in vivo complementation of a yeast HEM13 mutant., J Bacteriol 176(3):673-80

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