Note(s): |
SlyA activates hlyE trasnscription by antagonizing the negative action of the H-NS protein Ludwig A,1999. Wyborn NR,2004. Bartoli J,2020. H-NS prevents binding of RNA polymerase to the hlyE promoter. On the other hand, SlyA allows binding of RNA polymerase but inhibits binding of H-NS Lithgow JK,2007. The location of the sites for SlyA reported by Lithgow JK,2007 are unusual for typical bacterial transcription. However, it has been shown that some other S. typhimurium genes that are positively regulated by SlyA contain SlyA-binding sites downstream from the transcriptional start site, including pagC and ugtL Navarre WW, Halsey TA, Walthers D, Frye J, McClelland M, Potter JL, Kenney LJ, Gunn JS, Fang FC, Libby SJ,2005. Shi Y, Latifi T, Cromie MJ, Groisman EA,2004. FNR and CRP are in competition for binding at the hlyE regulatory region, and FNR ocuppies the site more frequently than CRP even under aerobic conditions, but under such conditions FNR is not an active protein and so this protein cannot activate trancription of hlyE as efficiently as CRP. The expression of hlyE CRP-mediated activation requires the presence of Hns. |
Sequence: |
Evidence: |
[HIPP] [TIM]
|
Reference(s): |
[2] Ludwig A., et al., 1999 [3] Westermark M., et al., 2000
|
Type |
Transcription factor |
Function |
Promoter |
Binding Sites |
Growth Conditions |
Evidence (Confirmed, Strong, Weak) |
Reference(s) |
LeftPos |
RightPos |
Central Rel-Pos |
Sequence |
proximal |
FNR
|
activator |
hlyEp |
1230521
|
1230534
|
-61.5
|
taaacattgtTTGATATTTATCATattaatagaa |
nd
|
[GEA], [BPP]
|
[3], [5]
|
Type |
Transcription factor |
Function |
Promoter |
Binding Sites |
Growth Conditions |
Evidence (Confirmed, Strong, Weak) |
Reference(s) |
LeftPos |
RightPos |
Central Rel-Pos |
Sequence |
proximal |
Fis
|
repressor |
hlyEp |
1230441
|
1230455
|
19.0
|
aagtaaaattTATTAATAGTTGTAAaacaggagtt |
nd
|
[GEA], [AIBSCS]
|
[4]
|
proximal |
Fis
|
repressor |
hlyEp |
1230451
|
1230465
|
9.0
|
gaattagatgAAGTAAAATTTATTAatagttgtaa |
nd
|
[GEA], [AIBSCS]
|
[4]
|
proximal |
Fis
|
repressor |
hlyEp |
1230522
|
1230536
|
-63.0
|
attaaacattGTTTGATATTTATCAtattaataga |
nd
|
[GEA], [AIBSCS]
|
[4]
|
remote |
Fis
|
repressor |
hlyEp |
1230569
|
1230583
|
-110.0
|
aatatcacccGGCTAAAAAATAGAAtagaagcatt |
nd
|
[GEA], [AIBSCS]
|
[4]
|
remote |
Fis
|
repressor |
hlyEp |
1230651
|
1230665
|
-192.0
|
tgcataggctGCTGATTTTTCGTTCtcttatcttg |
nd
|
[GEA], [AIBSCS]
|
[4]
|
Type |
Transcription factor |
Function |
Promoter |
Binding Sites |
Growth Conditions |
Evidence (Confirmed, Strong, Weak) |
Reference(s) |
LeftPos |
RightPos |
Central Rel-Pos |
Sequence |
proximal |
H-NS
|
activator |
hlyEp |
1230465
|
1230479
|
-6.0
|
gcccggctaaCTATGAATTAGATGAagtaaaattt |
nd
|
[GEA], [BPP]
|
[3], [5], [6]
|
proximal |
H-NS
|
activator |
hlyEp |
1230515
|
1230529
|
-56.5
|
attgtttgatATTTATCATATTAATagaaataaag |
nd
|
[GEA], [BPP]
|
[3], [5], [6]
|
Type |
Transcription factor |
Function |
Promoter |
Binding Sites |
Growth Conditions |
Evidence (Confirmed, Strong, Weak) |
Reference(s) |
LeftPos |
RightPos |
Central Rel-Pos |
Sequence |
proximal |
H-NS
|
repressor |
hlyEp |
1230465
|
1230479
|
-6.0
|
gcccggctaaCTATGAATTAGATGAagtaaaattt |
nd
|
[GEA], [BPP]
|
[3], [5], [6]
|
proximal |
H-NS
|
repressor |
hlyEp |
1230515
|
1230529
|
-56.5
|
attgtttgatATTTATCATATTAATagaaataaag |
nd
|
[GEA], [BPP]
|
[3], [5], [6]
|
Type |
Transcription factor |
Function |
Promoter |
Binding Sites |
Growth Conditions |
Evidence (Confirmed, Strong, Weak) |
Reference(s) |
LeftPos |
RightPos |
Central Rel-Pos |
Sequence |
remote |
SlyA
|
activator |
hlyEp |
1230376
|
1230377
|
90.5
|
aaatcgttgcAgataaaacgg |
nd
|
[GEA], [BPP]
|
[6], [7]
|
remote |
SlyA
|
activator |
hlyEp |
1230390
|
1230391
|
76.5
|
aatgattatgActgaaatcgt |
nd
|
[GEA], [BPP]
|
[6], [7]
|
remote |
SlyA
|
activator |
hlyEp |
1230405
|
1230406
|
61.5
|
atatttaaagAggcgaatgat |
nd
|
[GEA], [BPP]
|
[6], [7]
|
remote |
SlyA
|
activator |
hlyEp |
1230414
|
1230415
|
52.5
|
acaatttataTatttaaagag |
nd
|
[GEA], [BPP]
|
[6], [7]
|
proximal |
SlyA
|
activator |
hlyEp |
1230510
|
1230511
|
-44.5
|
tattaatagaAataaagacat |
nd
|
[GEA], [BPP]
|
[1], [2], [3], [5], [6], [7]
|
proximal |
SlyA
|
activator |
hlyEp |
1230521
|
1230522
|
-55.5
|
gatatttatcAtattaataga |
nd
|
[GEA], [BPP]
|
[2], [5], [6], [7]
|
Transcription factor |
Function |
Promoter |
Binding Sites |
Evidence (Confirmed, Strong, Weak) |
Reference(s) |
LeftPos |
RightPos |
Central Rel-Pos |
Sequence |
Fis |
repressor |
hlyEp |
1230651
|
1230665
|
-192.0
|
tgcataggctGCTGATTTTTCGTTCtcttatcttg
|
[GEA]
|
[4]
|
Fis |
repressor |
hlyEp |
1230569
|
1230583
|
-110.0
|
aatatcacccGGCTAAAAAATAGAAtagaagcatt
|
[GEA]
|
[4]
|
Fis |
repressor |
hlyEp |
1230522
|
1230536
|
-63.0
|
attaaacattGTTTGATATTTATCAtattaataga
|
[GEA]
|
[4]
|
CRP-cyclic-AMP |
activator |
hlyEp |
1230517
|
1230538
|
-61.5
|
acattaaacaTTGTTTGATATTTATCATATTAatagaaataa
|
[GEA]
|
[3], [5]
|
FNR |
activator |
hlyEp |
1230521
|
1230534
|
-61.5
|
taaacattgtTTGATATTTATCATattaatagaa
|
[GEA]
|
[3], [5]
|
H-NS |
activator |
hlyEp |
1230515
|
1230529
|
-56.5
|
attgtttgatATTTATCATATTAATagaaataaag
|
[GEA]
|
[3], [5], [6]
|
H-NS |
repressor |
hlyEp |
1230515
|
1230529
|
-56.5
|
attgtttgatATTTATCATATTAATagaaataaag
|
[GEA]
|
[3], [5], [6]
|
SlyA |
activator |
hlyEp |
1230521
|
1230522
|
-55.5
|
gatatttatcAtattaataga
|
[GEA]
|
[2], [5], [6], [7]
|
SlyA |
activator |
hlyEp |
1230510
|
1230511
|
-44.5
|
tattaatagaAataaagacat
|
[GEA]
|
[1], [2], [3], [5], [6], [7]
|
H-NS |
activator |
hlyEp |
1230465
|
1230479
|
-6.0
|
gcccggctaaCTATGAATTAGATGAagtaaaattt
|
[GEA]
|
[3], [5], [6]
|
H-NS |
repressor |
hlyEp |
1230465
|
1230479
|
-6.0
|
gcccggctaaCTATGAATTAGATGAagtaaaattt
|
[GEA]
|
[3], [5], [6]
|
Fis |
repressor |
hlyEp |
1230451
|
1230465
|
9.0
|
gaattagatgAAGTAAAATTTATTAatagttgtaa
|
[GEA]
|
[4]
|
Fis |
repressor |
hlyEp |
1230441
|
1230455
|
19.0
|
aagtaaaattTATTAATAGTTGTAAaacaggagtt
|
[GEA]
|
[4]
|
SlyA |
activator |
hlyEp |
1230414
|
1230415
|
52.5
|
acaatttataTatttaaagag
|
[GEA]
|
[6], [7]
|
SlyA |
activator |
hlyEp |
1230405
|
1230406
|
61.5
|
atatttaaagAggcgaatgat
|
[GEA]
|
[6], [7]
|
SlyA |
activator |
hlyEp |
1230390
|
1230391
|
76.5
|
aatgattatgActgaaatcgt
|
[GEA]
|
[6], [7]
|
SlyA |
activator |
hlyEp |
1230376
|
1230377
|
90.5
|
aaatcgttgcAgataaaacgg
|
[GEA]
|
[6], [7]
|
|