RegulonDB RegulonDB 10.7: Operon Form
   

glnK-amtB operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: glnK-amtB
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: glnK-amtB
Gene(s): glnK, amtB   Genome Browser M3D Gene expression COLOMBOS
Note(s): The transcription of the glnK-amtB operon is regulated by nitrogen availability; while nitrogen limitation causes operon expression, nitrogen excess represses completely the operon expression |CITS:[16740928]|.
Under nitrogen-rich growth conditions, the expression of the glnK gene increased in mutants for two genes that encode two terminal oxidases, cyoA and cydB, and in mutants for two transcriptional regulators, Fnr and Fur. However, under nitrogen-limited growth conditions, gene expression was decreased |CITS:[21272324]|.
Evidence: [BTEI] Boundaries of transcription experimentally identified
Reference(s): [1] van Heeswijk WC., et al., 1996
Promoter
Name: glnKp
+1: 472556
Sigma Factor: Sigma54 Sigmulon
Distance from start of the gene: 42
Sequence: ttaacttcctgctctctttctcgtttttcatttctggcacaccgcttgcaataccttcttCgtgtagcagaaccattaccg
                                   -24         -12          +1                   
Note(s): We assigned a putative transcription start site to this promoter based on the observation that the majority of the σ54-dependent promoters, whose transcription start sites were determined experimentally, present a distance of 11 nucleotides between the transcription start site and the -12 box Barrios H,1999.
Evidence: [AIPP]
[HIPP]
Reference(s): [2] Reitzer L., et al., 2001
[1] van Heeswijk WC., et al., 1996
Terminator(s)
Type: rho-independent
Sequence: caagcactgcAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTtaattgtgat
Reference(s): [1] van Heeswijk WC., et al., 1996
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd FNR activator glnKp nd nd nd nd nd [GEA] [4]
nd FNR repressor glnKp nd nd nd nd nd [GEA] [4]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd Fur-Fe2+ activator glnKp nd nd nd nd nd [GEA] [4]
nd Fur-Fe2+ repressor glnKp nd nd nd nd nd [GEA] [4]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd GadX activator glnKp nd nd nd nd nd [GEA] [3]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal NtrC-Pasp activator glnKp 472461 472477 -87.0 taacgcactgTGCACTGTCATAGTGCGttttcatttt nd [APIORCISFBSCS] [1]





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