RegulonDB RegulonDB 10.8: Operon Form
   

aroP operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: aroP
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: aroP
Gene(s): aroP   Genome Browser M3D Gene expression COLOMBOS
Note(s): The aroPp2 promoter is partially repressed by TyrR alone; this repression is enhanced in the presence of tyrosine or phenylalanine Yang J,1999 A lesser effect of repression of the aroP gene by tryptophan has also been observed Chye ML,1987 For the repression of aroPp2, an interaction between the N-terminal domain of TyrR and the RNA polymerase is required Yang J,1999
In the presence of tyrosine and ATP, the TyrR hexamer is bound to two tyrR boxes, a weak and a strong box located 43.5 and 20.5 bp downstream of the aroPp2 promoter, respectively. At these positions, TyrR represses the aroP gene expression, disabling the formation of the open complex Yang J,1999
In the presence of phenylalanine or in the absence of an effector, the TyrR dimer bound to the strong TryR box represses the transcription by inhibiting the step(s) that follows the formation of the open complex Yang J,1999
Evidence: [AISDTU] Automated inference that a single-gene directon is a transcription unit
[BTEI] Boundaries of transcription experimentally identified
Reference(s): [1] Honore N., et al., 1990
[2] Wang P., et al., 1997
Promoter
Name: aroPp2
+1: 121650
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 99
Sequence: tgcattcgctgccgcataccattattcttgatctgacggaagtctttttgtaacaattcaAacttctttgatgtaaacaaa
                            -35                     -10     +1                   
Note(s): aropP2 is the minor promoter that transcribes the aroP gene Wang P,1997
Evidence: [HIPP]
[TIM]
Reference(s): [2] Wang P., et al., 1997
Terminator(s)
Type: rho-independent
Sequence: aaagattattGCCCTCACCCTGTACGGGTGAGGGCgtagagagat
Reference(s): [1] Honore N., et al., 1990
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote Cra activator aroPp2 121840 121857 -198.5 taaaaatccgAATTGAACCGATTCACTTaccaattttg nd [AIBSCS], [CV(GEA)], [GEA], [ICWHO] [3], [4]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote TyrR-tyrosine1 repressor aroPp2 121600 121617 42.5 aatacaacaaACGGAATTGCAAACTTACacacgcatca nd [APIORCISFBSCS], [BPP], [CV(GEA)], [CV(GEA)], [CV(GEA/SM)], [CV(SM)], [GEA], [SM] [2], [5], [6], [7], [8], [9]
proximal TyrR-tyrosine2 repressor aroPp2 121623 121640 19.5 aacttctttgATGTAAACAAATTAATACaacaaacgga nd [APIORCISFBSCS], [BPP], [CV(GEA)], [CV(GEA)], [CV(GEA/SM)], [CV(SM)], [GEA], [SM] [2], [5], [6], [7], [8]
Note(s): 1The TyrR-binding site at position bp 20.5 from the aroPp2 promoter is a strong TyrR box that is involved in the repression of aroP gene transcription Yang J,1999 This strong box, which is closest to the promoter, is critical for repression, due to the fact that this site is bound by TyrR as a dimer or as a hexamer. The weak box has a less significant role in aroPp2 repression Yang J,1999
2The TyrR binding site at position 20.5 bp from the aroPp2 promoter is a strong TyrR box that is involved in the repression of aroP gene transcription
Yang J,1999 This strong box, which is closest to the
promoter, is critical for repression, due to the fact that this site is
bound by TyrR as a dimer or as a hexamer. The weak box has a less
significant role in the aroPp2 repression Yang J,19992The TyrR binding site at position 20.5 bp from the aroPp2 promoter is a strong TyrR box that is involved in the repression of aroP gene transcription
Yang J,1999 This strong box, which is closest to the
promoter, is critical for repression, due to the fact that this site is
bound by TyrR as a dimer or as a hexamer. The weak box has a less
significant role in the aroPp2 repression Yang J,1999
3The TyrR-binding site at position bp 20.5 from the aroPp2 promoter is a strong TyrR box that is involved in the repression of aroP gene transcription Yang J,1999 This strong box, which is closest to the promoter, is critical for repression, due to the fact that this site is bound by TyrR as a dimer or as a hexamer. The weak box has a less significant role in aroPp2 repression Yang J,1999


Transcription unit          
Name: aroP
Synonym(s): OP00014
Gene(s): aroP   Genome Browser M3D Gene expression COLOMBOS
Note(s): The transcription from the aroPp1 promoter is repressed by the TyrR
protein by recruiting the RNA polymerase to a nonproductive promoter
located divergently from the aroPp1 promoter overlapping it. The RNA
polymerase cannot be bound to both promoters at the same time. While
this repression can be observed in the presence of tyrosine, a lesser
repression is seen in the presence of phenylalanine or tryptophan
9765583. Chye ML,1987

TyrR binds to a strong and a weak TyrR box located at 40.5 and 63.5 bp
downstream of the aroPp1 promoter, respectively. The strong TyrR box is
directly involved in the repression of the aroPp1 promoter, although the
participation of the weak box has not been discarded Wang P,1998
Evidence: [AISDTU] Automated inference that a single-gene directon is a transcription unit
[BTEI] Boundaries of transcription experimentally identified
Reference(s): [5] Chye ML., et al., 1987
[1] Honore N., et al., 1990
[2] Wang P., et al., 1997
Promoter
Name: aroPp1
+1: 121671
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 120
Sequence: ttcaaccatgcataaagcgggtgcattcgctgccgcataccattattcttgatctgacggAagtctttttgtaacaattca
                     -35                    -10             +1                   
Note(s): aroPp1 is the major promoter transcribing the aroP gene. Although it has a weak -35 sequence, there is a UP element located upstream which increases its strength Wang P,1997
A nonproductive promoter called P3 is located divergently from the aroPp1 promoter, overlapping it. When the RNA polymerase is recruited by TyrR to this promoter the aroPp1 promoter remains inactive Wang P,1998 [9209034]|.
Evidence: [HIPP]
[TIM]
Reference(s): [5] Chye ML., et al., 1987
[2] Wang P., et al., 1997
Terminator(s)
Type: rho-independent
Sequence: aaagattattGCCCTCACCCTGTACGGGTGAGGGCgtagagagat
Reference(s): [1] Honore N., et al., 1990
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote Cra activator aroPp1 121840 121857 -177.5 taaaaatccgAATTGAACCGATTCACTTaccaattttg nd [AIBSCS], [CV(GEA)], [GEA] [3], [4]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote TyrR-tyrosine1 repressor aroPp1 121600 121617 63.5 aatacaacaaACGGAATTGCAAACTTACacacgcatca nd [APIORCISFBSCS], [BPP], [CV(GEA)], [CV(GEA)], [CV(GEA/SM)], [CV(SM)], [GEA], [SM] [2], [5], [6], [7], [8], [9]
remote TyrR-tyrosine2 repressor aroPp1 121623 121640 40.5 aacttctttgATGTAAACAAATTAATACaacaaacgga nd [APIORCISFBSCS], [BPP], [CV(GEA)], [CV(GEA)], [CV(GEA/SM)], [CV(SM)], [GEA], [SM] [2], [5], [6], [7], [8], [9]
Note(s): 1The TyrR-binding site at position 63.5 from the aroPp1 promoter is a weak TyrR box that is involved in the repression of aroP gene transcription Yang J,1999
2The TyrR-binding site at position bp 40.5 from the aroPp1 promoter is a strong TyrR box that is involved in the repression of aroP gene transcription Yang J,19992The TyrR-binding site at position bp 40.5 from the aroPp1 promoter is a strong TyrR box that is involved in the repression of aroP gene transcription Yang J,1999
3The TyrR-binding site at position 63.5 from the aroPp1 promoter is a weak TyrR box that is involved in the repression of aroP gene transcription Yang J,1999




Reference(s)    

 [1] Honore N., Cole ST., 1990, Nucleotide sequence of the aroP gene encoding the general aromatic amino acid transport protein of Escherichia coli K-12: homology with yeast transport proteins., Nucleic Acids Res 18(3):653

 [2] Wang P., Yang J., Pittard AJ., 1997, Promoters and transcripts associated with the aroP gene of Escherichia coli., J Bacteriol 179(13):4206-12

 [3] Sarkar D., Siddiquee KA., Arauzo-Bravo MJ., Oba T., Shimizu K., 2008, Effect of cra gene knockout together with edd and iclR genes knockout on the metabolism in Escherichia coli., Arch Microbiol 190(5):559-71

 [4] Shimada T., Yamamoto K., Ishihama A., 2011, Novel members of the Cra regulon involved in carbon metabolism in Escherichia coli., J Bacteriol 193(3):649-59

 [5] Chye ML., Pittard J., 1987, Transcription control of the aroP gene in Escherichia coli K-12: analysis of operator mutants., J Bacteriol 169(1):386-93

 [6] Wang JG., Fan CS., Wu YQ., Jin RL., Liu DX., Shang L., Jiang PH., 2003, Regulation of aroP expression by tyrR gene in Escherichia coli., Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) 35(11):993-7

 [7] Wang P., Yang J., Lawley B., Pittard AJ., 1997, Repression of the aroP gene of Escherichia coli involves activation of a divergent promoter., J Bacteriol 179(13):4213-8

 [8] Yang J., Wang P., Pittard AJ., 1999, Mechanism of repression of the aroP P2 promoter by the TyrR protein of Escherichia coli., J Bacteriol 181(20):6411-8

 [9] Wang P., Yang J., Ishihama A., Pittard AJ., 1998, Demonstration that the TyrR protein and RNA polymerase complex formed at the divergent P3 promoter inhibits binding of RNA polymerase to the major promoter, P1, of the aroP gene of Escherichia coli., J Bacteriol 180(20):5466-72


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