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| Operon |  |
|---|---|
| Name: | aroP |
| This page displays every known transcription unit of this operon and their known regulation. | |
| Name: | aroP |
| Gene(s): | aroP Genome Browser M3D Gene expression COLOMBOS |
| Note(s): | The aroPp2 promoter is partially repressed by TyrR alone; this repression is enhanced in the presence of tyrosine or phenylalanine Yang J,1999 A lesser effect of repression of the aroP gene by tryptophan has also been observed Chye ML,1987 For the repression of aroPp2, an interaction between the N-terminal domain of TyrR and the RNA polymerase is required Yang J,1999 In the presence of tyrosine and ATP, the TyrR hexamer is bound to two tyrR boxes, a weak and a strong box located 43.5 and 20.5 bp downstream of the aroPp2 promoter, respectively. At these positions, TyrR represses the aroP gene expression, disabling the formation of the open complex Yang J,1999 In the presence of phenylalanine or in the absence of an effector, the TyrR dimer bound to the strong TryR box represses the transcription by inhibiting the step(s) that follows the formation of the open complex Yang J,1999 |
| Evidence: | [AISDTU] Automated inference that a single-gene directon is a transcription unit [BTEI] Boundaries of transcription experimentally identified |
| Reference(s): |
[1] Honore N., et al., 1990 [2] Wang P., et al., 1997 |
| Promoter | |
| Name: | aroPp2 |
| +1: | 121650 |
| Sigma Factor: | Sigma70 Sigmulon |
| Distance from start of the gene: | 99 |
| Sequence: |
tgcattcgctgccgcataccattattcttgatctgacggaagtctttttgtaacaattcaAacttctttgatgtaaacaaa -35 -10 +1 |
| Note(s): | aropP2 is the minor promoter that transcribes the aroP gene Wang P,1997 |
| Evidence: |
[HIPP] [TIM] |
| Reference(s): | [2] Wang P., et al., 1997 |
| Terminator(s) | |
| Type: | rho-independent |
| Sequence: | aaagattattGCCCTCACCCTGTACGGGTGAGGGCgtagagagat |
| Reference(s): | [1] Honore N., et al., 1990 |
| Type | Transcription factor | Function | Promoter | Binding Sites | Growth Conditions | Evidence (Confirmed, Strong, Weak) | Reference(s) | |||
|---|---|---|---|---|---|---|---|---|---|---|
| LeftPos | RightPos | Central Rel-Pos | Sequence | |||||||
| remote | Cra | activator | aroPp2 | 121840 | 121857 | -198.5 | taaaaatccgAATTGAACCGATTCACTTaccaattttg | nd | [AIBSCS], [CV(GEA)], [GEA], [ICWHO] | [3], [4] |
| Type | Transcription factor | Function | Promoter | Binding Sites | Growth Conditions | Evidence (Confirmed, Strong, Weak) | Reference(s) | |||
|---|---|---|---|---|---|---|---|---|---|---|
| LeftPos | RightPos | Central Rel-Pos | Sequence | |||||||
| remote | TyrR-tyrosine1 | repressor | aroPp2 | 121600 | 121617 | 42.5 | aatacaacaaACGGAATTGCAAACTTACacacgcatca | nd | [APIORCISFBSCS], [BPP], [CV(GEA)], [CV(GEA)], [CV(GEA/SM)], [CV(SM)], [GEA], [SM] | [2], [5], [6], [7], [8], [9] |
| proximal | TyrR-tyrosine2 | repressor | aroPp2 | 121623 | 121640 | 19.5 | aacttctttgATGTAAACAAATTAATACaacaaacgga | nd | [APIORCISFBSCS], [BPP], [CV(GEA)], [CV(GEA)], [CV(GEA/SM)], [CV(SM)], [GEA], [SM] | [2], [5], [6], [7], [8] |
| Note(s): |
1The TyrR-binding site at position bp 20.5 from the aroPp2 promoter is a strong TyrR box that is involved in the repression of aroP gene transcription Yang J,1999 This strong box, which is closest to the promoter, is critical for repression, due to the fact that this site is bound by TyrR as a dimer or as a hexamer. The weak box has a less significant role in aroPp2 repression Yang J,1999 2The TyrR binding site at position 20.5 bp from the aroPp2 promoter is a strong TyrR box that is involved in the repression of aroP gene transcription Yang J,1999 This strong box, which is closest to the promoter, is critical for repression, due to the fact that this site is bound by TyrR as a dimer or as a hexamer. The weak box has a less significant role in the aroPp2 repression Yang J,19992The TyrR binding site at position 20.5 bp from the aroPp2 promoter is a strong TyrR box that is involved in the repression of aroP gene transcription Yang J,1999 This strong box, which is closest to the promoter, is critical for repression, due to the fact that this site is bound by TyrR as a dimer or as a hexamer. The weak box has a less significant role in the aroPp2 repression Yang J,1999 3The TyrR-binding site at position bp 20.5 from the aroPp2 promoter is a strong TyrR box that is involved in the repression of aroP gene transcription Yang J,1999 This strong box, which is closest to the promoter, is critical for repression, due to the fact that this site is bound by TyrR as a dimer or as a hexamer. The weak box has a less significant role in aroPp2 repression Yang J,1999 |
| Name: | aroP |
| Synonym(s): | OP00014 |
| Gene(s): | aroP Genome Browser M3D Gene expression COLOMBOS |
| Note(s): | The transcription from the aroPp1 promoter is repressed by the TyrR protein by recruiting the RNA polymerase to a nonproductive promoter located divergently from the aroPp1 promoter overlapping it. The RNA polymerase cannot be bound to both promoters at the same time. While this repression can be observed in the presence of tyrosine, a lesser repression is seen in the presence of phenylalanine or tryptophan 9765583. Chye ML,1987 TyrR binds to a strong and a weak TyrR box located at 40.5 and 63.5 bp downstream of the aroPp1 promoter, respectively. The strong TyrR box is directly involved in the repression of the aroPp1 promoter, although the participation of the weak box has not been discarded Wang P,1998 |
| Evidence: | [AISDTU] Automated inference that a single-gene directon is a transcription unit [BTEI] Boundaries of transcription experimentally identified |
| Reference(s): |
[5] Chye ML., et al., 1987 [1] Honore N., et al., 1990 [2] Wang P., et al., 1997 |
| Promoter | |
| Name: | aroPp1 |
| +1: | 121671 |
| Sigma Factor: | Sigma70 Sigmulon |
| Distance from start of the gene: | 120 |
| Sequence: |
ttcaaccatgcataaagcgggtgcattcgctgccgcataccattattcttgatctgacggAagtctttttgtaacaattca -35 -10 +1 |
| Note(s): | aroPp1 is the major promoter transcribing the aroP gene. Although it has a weak -35 sequence, there is a UP element located upstream which increases its strength Wang P,1997 A nonproductive promoter called P3 is located divergently from the aroPp1 promoter, overlapping it. When the RNA polymerase is recruited by TyrR to this promoter the aroPp1 promoter remains inactive Wang P,1998 [9209034]|. |
| Evidence: |
[HIPP] [TIM] |
| Reference(s): |
[5] Chye ML., et al., 1987 [2] Wang P., et al., 1997 |
| Terminator(s) | |
| Type: | rho-independent |
| Sequence: | aaagattattGCCCTCACCCTGTACGGGTGAGGGCgtagagagat |
| Reference(s): | [1] Honore N., et al., 1990 |
| Type | Transcription factor | Function | Promoter | Binding Sites | Growth Conditions | Evidence (Confirmed, Strong, Weak) | Reference(s) | |||
|---|---|---|---|---|---|---|---|---|---|---|
| LeftPos | RightPos | Central Rel-Pos | Sequence | |||||||
| remote | TyrR-tyrosine1 | repressor | aroPp1 | 121600 | 121617 | 63.5 | aatacaacaaACGGAATTGCAAACTTACacacgcatca | nd | [APIORCISFBSCS], [BPP], [CV(GEA)], [CV(GEA)], [CV(GEA/SM)], [CV(SM)], [GEA], [SM] | [2], [5], [6], [7], [8], [9] |
| remote | TyrR-tyrosine2 | repressor | aroPp1 | 121623 | 121640 | 40.5 | aacttctttgATGTAAACAAATTAATACaacaaacgga | nd | [APIORCISFBSCS], [BPP], [CV(GEA)], [CV(GEA)], [CV(GEA/SM)], [CV(SM)], [GEA], [SM] | [2], [5], [6], [7], [8], [9] |
| Note(s): |
1The TyrR-binding site at position 63.5 from the aroPp1 promoter is a weak TyrR box that is involved in the repression of aroP gene transcription Yang J,1999 2The TyrR-binding site at position bp 40.5 from the aroPp1 promoter is a strong TyrR box that is involved in the repression of aroP gene transcription Yang J,19992The TyrR-binding site at position bp 40.5 from the aroPp1 promoter is a strong TyrR box that is involved in the repression of aroP gene transcription Yang J,1999 3The TyrR-binding site at position 63.5 from the aroPp1 promoter is a weak TyrR box that is involved in the repression of aroP gene transcription Yang J,1999 |
| Reference(s) |
|
|---|---|
[HIPP] Human inference of promoter position