RegulonDB RegulonDB 10.6.3: Operon Form
   

zraSR operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: zraSR
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: zraSR
Synonym(s): hydHG
Gene(s): zraS, zraR   Genome Browser M3D Gene expression COLOMBOS
Evidence: [IEP] Inferred from expression pattern
[ITCR] Inferred through co-regulation
Reference(s): [1] Stoker K., et al., 1989
Promoter
Name: zraSp
+1: 4201905
Sigma Factor: Sigma54 Sigmulon
Distance from start of the gene: 21
Sequence: tgtgttacagcgcagggtaagcgctgataaaagatggcatgatttctgctgtcagaaaggGatgagcaggcaaagaagaag
                                   -24         -12          +1                   
Note(s): We assigned a putative transcription start site to this promoter based on the observation that the majority of the σ54-dependent promoters, whose transcription start sites were determined experimentally, present a distance of 11 nucleotides between the transcription start site and the -12 box Barrios H,1999.
Evidence: [AIPP]
[HIPP]
Reference(s): [2] Leonhartsberger S., et al., 2001
[3] Reitzer L., et al., 2001
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote CRP-cAMP activator zraSp 4201671 4201692 -223.5 ccagggcaatTTTCGTGTTCCGTTTCATGGTTaatcctccag nd [AIBSCS], [GEA] [4]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote ZraR-Phosphorylated activator zraSp 4201782 4201798 -115.0 catgataaacGAGTAAAAATGACTCGCctgctgcggg nd [BPP], [GEA], [HIBSCS] [2]
proximal ZraR-Phosphorylated2 activator zraSp 4201811 4201827 -86.0 gctgcgggtaGCGAGTCATTTTTACTCattgaaactt nd [BPP], [GEA] [2]
Note(s): 2ZraR activates the zraSp promoter in the presence of high Zn2+ and Pb2+ concentrationsLeonhartsberger S,2001.3ZraR activates the zraSp promoter in the presence of high Zn2+ and Pb2+ concentrationsLeonhartsberger S,2001.


RNA cis-regulatory element    
Regulation, transcriptional elongation  
Attenuator type: Transcriptional
Strand: forward
Evidence: [ICA] Inferred by computational analysis
Reference(s): [5] null null
  Structure type Energy LeftPos RightPos Sequence (RNA-strand)
  terminator -14.5 4201791 4201824 gagtaaaaatGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTActcattgaaa
  anti-terminator -5.3 4201770 4201803 gggtaatctgGCATGATAAACGAGTAAAAATGACTCGCCTGCTgcgggtagcg
  anti-anti-terminator -11.8 4201733 4201775 tgcatcttccGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGataaacgagt
Notes: "The provided "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appear as the reverse complement of the sequence delimited by LeftPos-RigtPos"





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