RegulonDB RegulonDB 10.8: Operon Form
   

queA operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: queA
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: queA
Gene(s): queA   Genome Browser M3D Gene expression COLOMBOS
Promoter
Name: queAp
+1: 424985
Distance from start of the gene: 26
Sequence: tattctatccaaaagggggtaaaggttgcagggagagcgccccggcactagactacccgcCtcttattttagtctgagtca
Evidence: [CV(RS-EPT-CBR)]
[CV(TIM)]
[HIPP]
[RS-EPT-CBR]
[TIM]
Reference(s): [1] Reuter K., et al., 1991
[2] Salgado H, et al., 2012
TF binding sites (TFBSs)
[Fis,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal Fis activator queAp 424920 424934 -58.0 aaaattcatcGATTATATTCTATCCaaaagggggt nd [BPP], [GEA] [3]
Allosteric regulation of RNA-polymerase
  Regulator Function Promoter target of RNApol Growth Conditions Note Evidence Reference
  ppGpp inhibition queAp   [EME] [4]
Evidence: [EME] Expression microarray evidence
Reference(s): [4] Traxler MF., et al., 2008


Evidence    

 [CV(RS-EPT-CBR)] cross validation(RS-EPT-CBR)

 [CV(TIM)] cross validation(TIM)

 [HIPP] Human inference of promoter position

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [TIM] Transcription initiation mapping

 [BPP] Binding of purified proteins

 [GEA] Gene expression analysis


Reference(s)    

 [1] Reuter K., Slany R., Ullrich F., Kersten H., 1991, Structure and organization of Escherichia coli genes involved in biosynthesis of the deazaguanine derivative queuine, a nutrient factor for eukaryotes., J Bacteriol 173(7):2256-64

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [3] Slany RK., Kersten H., 1992, The promoter of the tgt/sec operon in Escherichia coli is preceded by an upstream activation sequence that contains a high affinity FIS binding site., Nucleic Acids Res 20(16):4193-8

 [4] Traxler MF., Summers SM., Nguyen HT., Zacharia VM., Hightower GA., Smith JT., Conway T., 2008, The global, ppGpp-mediated stringent response to amino acid starvation in Escherichia coli., Mol Microbiol 68(5):1128-48

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