RegulonDB RegulonDB 10.6.3: Operon Form
   

rcsA operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: rcsA
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: rcsA
Gene(s): rcsA   Genome Browser M3D Gene expression COLOMBOS
Note(s): Both sdiA and rcsA are involved in acyl-homoserine lactone (AHL)-mediated induction of the prophage process, and this induction is RecA independent |CITS:[19783745]|. Based on analysis with mutants, sdiA does not have any direct negative or positive regulatory role in rcsA expression, and the data suggest that intermediate transducers are involved in carrying the AHL-SdiA-mediated signal to activate rcsA for prophage induction |CITS:[19783745]|.
The expression of the gene rcsA is increased when colicin (at 30 ng/ml) is added to the medium |CITS: [23421615]|.
Evidence: [LTED] Length of transcript experimentally determined
Reference(s): [1] Ebel W., et al., 1999
Promoter
Name: rcsAp
+1: 2023836
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 132
Sequence: ctcactcacatatcgcaacatttactttactttaagacaattccaggcaaattatacaacActttacgggatagtaagtcc
                          -35                     -10       +1                   
Evidence: [HIPP]
[TIM]
Reference(s): [2] Sledjeski D., et al., 1995
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd GadE activator rcsAp nd nd nd nd nd [BPP], [GEA] [4]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd H-NS1 repressor rcsAp nd nd nd nd nd [GEA], [ICWHO] [1], [2], [5], [6]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote RcsAB activator rcsAp 2023657 2023671 -172.0 ctgatattatTAGTGAATTGCCATTaatataattc nd [AIBSCS], [GEA] [1]
remote RcsAB2 activator rcsAp 2023704 2023718 -125.0 catgttatccTAAGGATTATCCGAAaaataatacc nd [BCE], [BPP], [SM] [3]
Note(s): 1Beyond the silencing of rcsA expression by H-NS and the multicopy effect of DsrA on H-NS, little is known about the regulation of rscA expression.
Based on bioinformatics analyses with MODEL.IT and PYMOL software, it was determined that the region -130 to -400, from the TSS of rcsAp, does exist as a putative curved DNA and could be the most probable region where the H-NS binds to and represses rcsA transcription Meenakshi S,20182The effect of RcsB on rcsA expression depends on the presence of the RcsA protein. There is a putative Rcs box (RcsA-RcsB)-binding site in the rcsA promoter region. Direct interaction of RcsB and/or RcsA in the E. coli rcsA promoter region remains to be shown. The RcsB effect on rcsA expression can be overcome by excess RcsA, indicating that RcsB functions as an auxiliary factor in rcsA expression.2The effect of RcsB on rcsA expression depends on the presence of the RcsA protein. There is a putative Rcs box (RcsA-RcsB)-binding site in the rcsA promoter region. Direct interaction of RcsB and/or RcsA in the E. coli rcsA promoter region remains to be shown. The RcsB effect on rcsA expression can be overcome by excess RcsA, indicating that RcsB functions as an auxiliary factor in rcsA expression.
4Beyond the silencing of rcsA expression by H-NS and the multicopy effect of DsrA on H-NS, little is known about the regulation of rscA expression.
Based on bioinformatics analyses with MODEL.IT and PYMOL software, it was determined that the region -130 to -400, from the TSS of rcsAp, does exist as a putative curved DNA and could be the most probable region where the H-NS binds to and represses rcsA transcription Meenakshi S,2018





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