RegulonDB RegulonDB 11.1: Operon Form
   

aspV operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: aspV
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: aspV
Gene(s): aspV   Genome Browser M3D Gene expression COLOMBOS
Evidence: [EXP-IDA-BOUNDARIES-DEFINED] Boundaries of transcription experimentally identified
Promoter
Name: aspVp
+1: 236913
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 18
Sequence: ttcaaaagatgagaaaaaccgttgacgaaggtcgaggcaatccgtaatattcgcctcgttCccaacggaacacaacgcgga
                      -35                    -10            +1                   
Evidence: [COMP-AINF]
[COMP-HINF-POSITIONAL-IDENTIFICATION]
Reference(s): [1] Huerta AM., et al., 2003
Terminator(s)
Type: rho-independent
Sequence: aaaatgagttCAGAGAGCCGCAAGATTTTTAATTTTGCGGTTTTTTTgtatttgaat
Reference(s): [2] Cox EC., et al., 1986
[3] Peters JM., et al., 2009
[4] Zuker M. 2003
TF binding sites (TFBSs)
[Fis,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence Confidence level (C: Confirmed, S: Strong, W: Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal Fis activator aspVp 236830 236844 -76.0 aatagcgactTGGGCGATTTTTGCAgcaaacgatt nd [EXP-IDA-BINDING-OF-CELLULAR-EXTRACTS] nd nd


Evidence    

 [COMP-AINF] Inferred computationally without human oversight

 [COMP-HINF-POSITIONAL-IDENTIFICATION] Human inference of promoter position

 [EXP-IDA-BINDING-OF-CELLULAR-EXTRACTS] Binding of cellular extracts


Reference(s)    

 [1] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [2] Cox EC., Horner DL., 1986, DNA sequence and coding properties of mutD(dnaQ) a dominant Escherichia coli mutator gene., J Mol Biol 190(1):113-7

 [3] Peters JM., Mooney RA., Kuan PF., Rowland JL., Keles S., Landick R., 2009, Rho directs widespread termination of intragenic and stable RNA transcription., Proc Natl Acad Sci U S A 106(36):15406-11

 [4] Zuker M., 2003, Mfold web server for nucleic acid folding and hybridization prediction., Nucleic Acids Res 31(13):3406-15

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