RegulonDB RegulonDB 10.8: Operon Form
   

valUXY-lysV operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: valUXY-lysV
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: valUXY-lysV
Synonym(s): valU
Gene(s): valU, valX, valY, lysV   Genome Browser M3D Gene expression COLOMBOS
Evidence: [BTEI] Boundaries of transcription experimentally identified
Promoter
Name: valUp
+1: 2520924
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 7
Sequence: aacaatttctttagaaaatgcgttgactcattttgaactctccctataatgcgactccacAcagcgggggtgattagctca
                       -35                   -10            +1                   
Evidence: [HIPP]
Terminator(s)
Type: rho-independent
Sequence: cgacccaccaATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTAtctgcgattt
Reference(s): [1] Peters JM., et al., 2009
[2] Zuker M. 2003
TF binding sites (TFBSs)
[Fis,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote Fis activator valUp 2520803 2520817 -114.0 tattgttttcGTTGACAAATTGCGCaacacggtct nd [AIBSCS], [BPP], [CV(CHIP-SV/SM)], [SM] [3]
remote Fis activator valUp 2520824 2520838 -93.0 gcgcaacacgGTCTGTTTTGCGATCatcttgctta nd [AIBSCS], [APIORCISFBSCS], [BPP], [CV(CHIP-SV/SM)], [SM] [3]
proximal Fis activator valUp 2520844 2520858 -73.0 cgatcatcttGCTTAAATTTACGACgaacgaacaa nd [AIBSCS], [BCE], [BPP], [CV(CHIP-SV/SM)], [SM] [3]


Evidence    

 [HIPP] Human inference of promoter position

 [AIBSCS] Automated inference based on similarity to consensus sequences

 [BPP] Binding of purified proteins

 [CV(CHIP-SV/SM)] cross validation(CHIP-SV/SM)

 [SM] Site mutation

 [APIORCISFBSCS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.

 [BCE] Binding of cellular extracts


Reference(s)    

 [1] Peters JM., Mooney RA., Kuan PF., Rowland JL., Keles S., Landick R., 2009, Rho directs widespread termination of intragenic and stable RNA transcription., Proc Natl Acad Sci U S A 106(36):15406-11

 [2] Zuker M., 2003, Mfold web server for nucleic acid folding and hybridization prediction., Nucleic Acids Res 31(13):3406-15

 [3] Champagne N., Lapointe J., 1998, Influence of FIS on the transcription from closely spaced and non-overlapping divergent promoters for an aminoacyl-tRNA synthetase gene (gltX) and a tRNA operon (valU) in Escherichia coli., Mol Microbiol 27(6):1141-56

RegulonDB