RegulonDB RegulonDB 10.8: Operon Form
   

metT-leuW-glnUW-metU-glnVX operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: metT-leuW-glnUW-metU-glnVX
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: metT-leuW-glnUW-metU-glnVX
Synonym(s): metT
Gene(s): glnX, glnV, metU, glnW, glnU, leuW, metT   Genome Browser M3D Gene expression COLOMBOS
Note(s): This operon overlaps with three ORFs in the opposite direction.
Evidence: [BTEI] Boundaries of transcription experimentally identified
[LTED] Length of transcript experimentally determined
Reference(s): [1] Mohanty BK., et al., 2008
[2] Nakajima N., et al., 1981
[3] Nakajima N., et al., 1982
Promoter
Name: metTp
+1: 697163
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 30
Sequence: caattttccttgaaaaagaggttgacgctgcaaggctctatacgcataatgcgccccgcaAcgccgataaggtatcgcgaa
                      -35                    -10            +1                   
Evidence: [HIPP]
Terminator(s)
Type: rho-independent
Sequence: aatgaacgcaGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGtggctggggt
Reference(s): [4] Fournier MJ., et al., 1985
[5] Peters JM., et al., 2009
[6] Zuker M. 2003
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal Fis activator metTp 697223 697237 -67.0 cctaaccaaaCAGTCACTTTCGAGCaattttcctt nd [IHBCE] nd
Allosteric regulation of RNA-polymerase
  Regulator Function Promoter target of RNApol Growth Conditions Note Evidence Reference
  ppGpp inhibition metTp Based on results of nuclease protection and primer extension assays, leuV, argT, metT, and leuX were determined to be involved in the stringent response induced by serine hydroxamate Rowley KB,1993. [IEP] [7]
Evidence: [IEP] Inferred from expression pattern
Reference(s): [7] Rowley KB., et al., 1993


Transcription unit       
Name: glnW-metU-glnVX
Gene(s): glnX, glnV, metU, glnW   Genome Browser M3D Gene expression COLOMBOS
Promoter
Name: glnWp
+1: Unknown
Note(s): Zaslaver et al. demonstrated in 2006, by means of a library of fluorescent transcription fusions, that this promoter can be transcribed in vitro Zaslaver A,2006. Based on this, a putative promoter was suggested, but the +1 site of the transcription initiation has not been determined, although there exists promoter activity.
Evidence: [IEP]
Reference(s): [8] Zaslaver A., et al., 2006
Terminator(s)
Type: rho-independent
Sequence: aatgaacgcaGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGtggctggggt
Reference(s): [4] Fournier MJ., et al., 1985
[5] Peters JM., et al., 2009
[6] Zuker M. 2003





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