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Name: | metT-leuW-glnUW-metU-glnVX |
Synonym(s): | metT |
Gene(s): | glnX, glnV, metU, glnW, glnU, leuW, metT Genome Browser M3D Gene expression COLOMBOS |
Note(s): | This operon overlaps with three ORFs in the opposite direction. |
Evidence: | [BTEI] Boundaries of transcription experimentally identified [LTED] Length of transcript experimentally determined |
Reference(s): |
[1] Mohanty BK., et al., 2008 [2] Nakajima N., et al., 1981 [3] Nakajima N., et al., 1982 |
Promoter | |
Name: | metTp |
+1: | 697163 |
Sigma Factor: | Sigma70 Sigmulon |
Distance from start of the gene: | 30 |
Sequence: |
caattttccttgaaaaagaggttgacgctgcaaggctctatacgcataatgcgccccgcaAcgccgataaggtatcgcgaa -35 -10 +1 |
Evidence: | [HIPP] |
Terminator(s) | |
Type: | rho-independent |
Sequence: | aatgaacgcaGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGtggctggggt |
Reference(s): |
[4] Fournier MJ., et al., 1985 [5] Peters JM., et al., 2009 [6] Zuker M. 2003 |
Allosteric regulation of RNA-polymerase |
Regulator | Function | Promoter target of RNApol | Growth Conditions | Note | Evidence | Reference | |
---|---|---|---|---|---|---|---|
ppGpp | inhibition | metTp | Based on results of nuclease protection and primer extension assays, leuV, argT, metT, and leuX were determined to be involved in the stringent response induced by serine hydroxamate Rowley KB,1993. | [IEP] | [7] |
Evidence: | [IEP] Inferred from expression pattern |
Reference(s): | [7] Rowley KB., et al., 1993 |
Name: | glnW-metU-glnVX |
Gene(s): | glnX, glnV, metU, glnW Genome Browser M3D Gene expression COLOMBOS |
Promoter | |
Name: | glnWp |
+1: | Unknown |
Note(s): | Zaslaver et al. demonstrated in 2006, by means of a library of fluorescent transcription fusions, that this promoter can be transcribed in vitro Zaslaver A,2006. Based on this, a putative promoter was suggested, but the +1 site of the transcription initiation has not been determined, although there exists promoter activity. |
Evidence: | [IEP] |
Reference(s): | [8] Zaslaver A., et al., 2006 |
Terminator(s) | |
Type: | rho-independent |
Sequence: | aatgaacgcaGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGtggctggggt |
Reference(s): |
[4] Fournier MJ., et al., 1985 [5] Peters JM., et al., 2009 [6] Zuker M. 2003 |
Reference(s) |
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