RegulonDB RegulonDB 11.1: Operon Form
   

alaWX operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: alaWX
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: alaWX
Synonym(s): alaW
Gene(s): alaX, alaW   Genome Browser M3D Gene expression COLOMBOS
Evidence: [EXP-IDA-BOUNDARIES-DEFINED] Boundaries of transcription experimentally identified
Promoter
Name: alaWp
+1: 2518253
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 22
Sequence: actacacgttgcaatttgccgttgacacattcgggcggaattcatatgatgccgcccgtcAactcgacaagctttacgtga
                           -35                    -10       +1                   
Evidence: [COMP-AINF]
[COMP-HINF-POSITIONAL-IDENTIFICATION]
Reference(s): [1] Huerta AM., et al., 2003
Terminator(s)
Type: rho-independent
Sequence: caaaaagcagAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTggtggagcta
Reference(s): [2] Peters JM., et al., 2009
[3] Zuker M. 2003
TF binding sites (TFBSs)
[Fis,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence Confidence level (C: Confirmed, S: Strong, W: Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal Fis activator alaWp 2518322 2518336 -76.0 attgttcattTGCCTGATTTTTCAGcgattgaaac nd [EXP-IDA-BINDING-OF-CELLULAR-EXTRACTS] nd nd


RNA cis-regulatory element    
Regulation, transcriptional elongation  
Attenuator type: Translational
Strand: reverse
  Structure type Energy LeftPos RightPos Sequence (RNA-strand)
  terminator -9.0 2518229 2518259 tatgatgccgCCCGTCAACTCGACAAGCTTTACGTGACGGggctatagct
Notes: "The provided "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appear as the reverse complement of the sequence delimited by LeftPos-RigtPos"


Evidence    

 [COMP-AINF] Inferred computationally without human oversight

 [COMP-HINF-POSITIONAL-IDENTIFICATION] Human inference of promoter position

 [EXP-IDA-BINDING-OF-CELLULAR-EXTRACTS] Binding of cellular extracts


Reference(s)    

 [1] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [2] Peters JM., Mooney RA., Kuan PF., Rowland JL., Keles S., Landick R., 2009, Rho directs widespread termination of intragenic and stable RNA transcription., Proc Natl Acad Sci U S A 106(36):15406-11

 [3] Zuker M., 2003, Mfold web server for nucleic acid folding and hybridization prediction., Nucleic Acids Res 31(13):3406-15

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