RegulonDB RegulonDB 11.1: Operon Form
   

pheU operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: pheU
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: pheU
Gene(s): pheU   Genome Browser M3D Gene expression COLOMBOS
Evidence: [EXP-IDA-BOUNDARIES-DEFINED] Boundaries of transcription experimentally identified
[EXP-IDA-TRANSCRIPT-LEN-DETERMINATION] Length of transcript experimentally determined
Reference(s): [1] Ow MC., et al., 2002
Promoter
Name: pheUp
+1: 4362627
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 1
Sequence: aatcgcttttactgaaattaggttgacgagatgtgcagattacggtttaatgcgccccgtTgcccggatagctcagtcggt
                            -35                      -10    +1                   
Evidence: [COMP-AINF]
[COMP-HINF-POSITIONAL-IDENTIFICATION]
Reference(s): [2] Huerta AM., et al., 2003
Terminator(s)
Type: rho-independent
Sequence: tggcgtcctgAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAAtttggtgccc
Reference(s): [3] Peters JM., et al., 2009
[4] Zuker M. 2003
TF binding sites (TFBSs)
[Fis,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence Confidence level (C: Confirmed, S: Strong, W: Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal Fis activator pheUp 4362694 4362708 -74.0 agaagcgattTGCCGCAATCTTAAGcagttgaatc nd [COMP-HINF] nd nd


Evidence    

 [COMP-AINF] Inferred computationally without human oversight

 [COMP-HINF-POSITIONAL-IDENTIFICATION] Human inference of promoter position

 [COMP-HINF] Inferred by a human based on computational evidence


Reference(s)    

 [1] Ow MC., Kushner SR., 2002, Initiation of tRNA maturation by RNase E is essential for cell viability in E. coli., Genes Dev 16(9):1102-15

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [3] Peters JM., Mooney RA., Kuan PF., Rowland JL., Keles S., Landick R., 2009, Rho directs widespread termination of intragenic and stable RNA transcription., Proc Natl Acad Sci U S A 106(36):15406-11

 [4] Zuker M., 2003, Mfold web server for nucleic acid folding and hybridization prediction., Nucleic Acids Res 31(13):3406-15

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