RegulonDB RegulonDB 10.8: Operon Form
   

putP operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: putP
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: putP
Gene(s): putP   Genome Browser M3D Gene expression COLOMBOS
Note(s): Under NlpE outer membrane lipoprotein overexpression, the transcription of the putP gene is decreased Raivio TL,2013
Evidence: [BTEI] Boundaries of transcription experimentally identified
Reference(s): [1] Nakao T., et al., 1987
[2] Nakao T., et al., 1987
Promoter
Name: putPp1
+1: 1079168
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 137
Sequence: gagtgcaacctgatgaaaaatagtgtcgctgagcactaaaatttaatgtaaatggtgtgtTaaatcgattgtgaataacca
                       -35                  -10             +1                   
Evidence: [HIPP]
[TIM]
Reference(s): [2] Nakao T., et al., 1987
Terminator(s)
Type: rho-independent
Sequence: gctccgccgtCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTttggcttcag
Reference(s): [1] Nakao T., et al., 1987
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal PutA repressor putPp1 1079084 1079089 -81.5 atttttgaaaGGTGCAaccgcaaaaa nd [BPP], [CV(SM)], [GEA], [SM] [3]
proximal PutA repressor putPp1 1079111 1079116 -54.5 tgtgagagagTGCAACctgatgaaaa nd [BPP], [CV(SM)], [GEA], [SM] [3]


Transcription unit       
Name: putP
Gene(s): putP   Genome Browser M3D Gene expression COLOMBOS
Reference(s): [1] Nakao T., et al., 1987
[2] Nakao T., et al., 1987
Promoter
Name: putPp2
+1: 1079179
Distance from start of the gene: 126
Sequence: gatgaaaaatagtgtcgctgagcactaaaatttaatgtaaatggtgtgttaaatcgattgTgaataaccagcgcttccggc
Evidence: [TIM]
Reference(s): [2] Nakao T., et al., 1987
Terminator(s)
Type: rho-independent
Sequence: gctccgccgtCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTttggcttcag
Reference(s): [1] Nakao T., et al., 1987


Transcription unit       
Name: putP
Gene(s): putP   Genome Browser M3D Gene expression COLOMBOS
Evidence: [BTEI] Boundaries of transcription experimentally identified
Reference(s): [1] Nakao T., et al., 1987
[2] Nakao T., et al., 1987
Promoter
Name: putPp3
+1: 1079185
Distance from start of the gene: 120
Sequence: aaatagtgtcgctgagcactaaaatttaatgtaaatggtgtgttaaatcgattgtgaataAccagcgcttccggcaggata
Evidence: [TIM]
Reference(s): [2] Nakao T., et al., 1987
Terminator(s)
Type: rho-independent
Sequence: gctccgccgtCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTttggcttcag
Reference(s): [1] Nakao T., et al., 1987


Transcription unit          
Name: putP
Gene(s): putP   Genome Browser M3D Gene expression COLOMBOS
Evidence: [BTEI] Boundaries of transcription experimentally identified
Reference(s): [1] Nakao T., et al., 1987
[2] Nakao T., et al., 1987
Promoter
Name: putPp4
+1: 1079210
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 95
Sequence: ttaatgtaaatggtgtgttaaatcgattgtgaataaccagcgcttccggcaggatacggtCgccctggtaaaacataaact
                           -35                    -10       +1                   
Evidence: [HIPP]
[TIM]
Reference(s): [1] Nakao T., et al., 1987
[2] Nakao T., et al., 1987
Terminator(s)
Type: rho-independent
Sequence: gctccgccgtCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTttggcttcag
Reference(s): [1] Nakao T., et al., 1987
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote CRP-cAMP repressor putPp4 1079093 1079114 -106.0 aggtgcaaccGCAAAAAATGTGAGAGAGTGCAacctgatgaa nd [APIORCISFBSCS], [CV(GEA)], [GEA] [1]
proximal CRP-cAMP repressor putPp4 1079123 1079144 -76.5 caacctgatgAAAAATAGTGTCGCTGAGCACTaaaatttaat nd [APIORCISFBSCS], [CV(GEA)], [GEA] [1]
proximal CRP-cAMP repressor putPp4 1079155 1079176 -44.5 aaaatttaatGTAAATGGTGTGTTAAATCGATtgtgaataac nd [APIORCISFBSCS], [CV(GEA)], [GEA] [1]


Transcription unit       
Name: putP
Gene(s): putP   Genome Browser M3D Gene expression COLOMBOS
Evidence: [BTEI] Boundaries of transcription experimentally identified
Reference(s): [1] Nakao T., et al., 1987
[2] Nakao T., et al., 1987
Promoter
Name: putPp5
+1: 1079291
Distance from start of the gene: 14
Sequence: ctgttaccccgttccggtggcagatataacggcaagtttcgacattgccgataataatttTttggagactttagatggcta
Evidence: [CV(RS-EPT-CBR)]
[CV(TIM)]
[RS-EPT-CBR]
[TIM]
Reference(s): [2] Nakao T., et al., 1987
[4] Salgado H, et al., 2012
Terminator(s)
Type: rho-independent
Sequence: gctccgccgtCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTttggcttcag
Reference(s): [1] Nakao T., et al., 1987


RNA cis-regulatory element    
Regulation, transcriptional elongation  
Attenuator type: Translational
Strand: forward
  Structure type Energy LeftPos RightPos Sequence (RNA-strand)
  terminator -7.5 1079260 1079280 cagatataacGGCAAGTTTCGACATTGCCGataataattt
Notes: "The provided "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appear as the reverse complement of the sequence delimited by LeftPos-RigtPos"





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