RegulonDB RegulonDB 10.9: Operon Form
   

putA operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: putA
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: putA
Gene(s): putA   Genome Browser M3D Gene expression COLOMBOS
Reference(s): [1] Nakao T., et al., 1987
Promoter
Name: putAp
+1: 1078925
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 43
Sequence: ttgctacgcatgtcacatttaacatggttgcacaaagttgcaacatcatggatatttcacGataacgttaagttgcacctt
                            -35                    -10      +1                   
Evidence: [HIPP]
[ICWHO]
[TIM]
Reference(s): [2] Huerta AM., et al., 2003
[1] Nakao T., et al., 1987
TF binding sites (TFBSs)
[BasR,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal BasR-Phosphorylated repressor putAp 1078914 1078933 2.5 aacatcatggATATTTCACGATAACGTTAAGttgcaccttt nd [APIORCISFBSCS], [BPP] [6]
[MarA,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd MarA activator putAp nd nd nd nd nd [BPP], [GEA], [IGI] [7]
[PutA,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal PutA repressor putAp 1078909 1078914 14.5 gataacgttaAGTTGCAcctttctgaa nd [BPP], [GEA], [SM] [5]
proximal PutA repressor putAp 1078944 1078949 -21.5 ggttgcacaaAGTTGCAacatcatgga nd [BPP], [GEA], [SM] [5]
proximal PutA repressor putAp 1078954 1078959 -31.5 catttaacatGGTTGCAcaaagttgca nd [BPP], [GEA], [SM] [3], [4], [5]


Evidence    

 [HIPP] Human inference of promoter position

 [ICWHO] Inferred computationally without human oversight

 [TIM] Transcription initiation mapping

 [APIORCISFBSCS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.

 [BPP] Binding of purified proteins

 [GEA] Gene expression analysis

 [IGI] Inferred from genetic interaction

 [SM] Site mutation


Reference(s)    

 [1] Nakao T., Yamato I., Anraku Y., 1987, Nucleotide sequence of putC, the regulatory region for the put regulon of Escherichia coli K12., Mol Gen Genet 210(2):364-8

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [3] Becker DF., Thomas EA., 2001, Redox properties of the PutA protein from Escherichia coli and the influence of the flavin redox state on PutA-DNA interactions., Biochemistry 40(15):4714-21

 [4] Brown ED., Wood JM., 1992, Redesigned purification yields a fully functional PutA protein dimer from Escherichia coli., J Biol Chem 267(18):13086-92

 [5] Zhou Y., Larson JD., Bottoms CA., Arturo EC., Henzl MT., Jenkins JL., Nix JC., Becker DF., Tanner JJ., 2008, Structural basis of the transcriptional regulation of the proline utilization regulon by multifunctional PutA., J Mol Biol 381(1):174-88

 [6] Ogasawara H., Shinohara S., Yamamoto K., Ishihama A., 2012, Novel regulation targets of the metal-response BasS-BasR two-component system of Escherichia coli., Microbiology 158(Pt 6):1482-92

 [7] Pomposiello PJ., Bennik MH., Demple B., 2001, Genome-wide transcriptional profiling of the Escherichia coli responses to superoxide stress and sodium salicylate., J Bacteriol 183(13):3890-902

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