RegulonDB RegulonDB 10.8: Operon Form
   

potFGHI operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: potFGHI
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit       
Name: potFGHI
Gene(s): potF, potG, potH, potI   Genome Browser M3D Gene expression COLOMBOS
Note(s): There is a putative weak transcriptional termination signal in the potG gene, so synthesis of a part of mRNA may stop at this point.
Evidence: [LTED] Length of transcript experimentally determined
Reference(s): [1] Pistocchi R., et al., 1993
Promoter
Name: potFp2
+1: 893634
Distance from start of the gene: 150
Sequence: atttgttaaataccgttttttaatccgagctatagtctcaaaccctggctaaagttattcTtgcgatgcttttatatagcg
Evidence: [TIM]
Reference(s): [1] Pistocchi R., et al., 1993


Transcription unit          
Name: potFGHI
Gene(s): potF, potG, potH, potI   Genome Browser M3D Gene expression COLOMBOS
Note(s): There is a putative weak transcriptional termination signal in the potG gene, so synthesis of a part of mRNA may stop at this point.
Evidence: [LTED] Length of transcript experimentally determined
Reference(s): [1] Pistocchi R., et al., 1993
Promoter
Name: potFp1
+1: 893736
Sigma Factor: Sigma54 Sigmulon
Distance from start of the gene: 48
Sequence: gttctcttttcttacaccgcgccgataaaaaatatgcacgtttattgcatatctttcagtGtgacaacttttgttcgtttg
                                  -24         -12           +1                   
Note(s): We assigned a putative transcription start site to this promoter based on the observation that the majority of the σ54-dependent promoters, whose transcription start sites were determined experimentally, present a distance of 11 nucleotides between the transcription start site and the -12 box Barrios H,1999.
Evidence: [AIPP]
Reference(s): [2] Reitzer L., et al., 2001
TF binding sites (TFBSs)
[NtrC,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd NtrC-Pasp activator potFp1 nd nd nd nd nd [GEA] [3]


Evidence    

 [TIM] Transcription initiation mapping

 [AIPP] Automated inference of promoter position

 [GEA] Gene expression analysis


Reference(s)    

 [1] Pistocchi R., Kashiwagi K., Miyamoto S., Nukui E., Sadakata Y., Kobayashi H., Igarashi K., 1993, Characteristics of the operon for a putrescine transport system that maps at 19 minutes on the Escherichia coli chromosome., J Biol Chem 268(1):146-52

 [2] Reitzer L., Schneider BL., 2001, Metabolic context and possible physiological themes of sigma(54)-dependent genes in Escherichia coli., Microbiol Mol Biol Rev 65(3):422-44, table of contents

 [3] Zimmer DP., Soupene E., Lee HL., Wendisch VF., Khodursky AB., Peter BJ., Bender RA., Kustu S., 2000, Nitrogen regulatory protein C-controlled genes of Escherichia coli: scavenging as a defense against nitrogen limitation., Proc Natl Acad Sci U S A 97(26):14674-9

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