RegulonDB RegulonDB 10.8: Operon Form
   

ydjA-selD-topB operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: ydjA-selD-topB
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit       
Name: selD-topB
Gene(s): topB, selD   Genome Browser M3D Gene expression COLOMBOS
Evidence: [BTEI] Boundaries of transcription experimentally identified
[LTED] Length of transcript experimentally determined
Reference(s): [1] Sawers G., et al., 1991
Promoter
Name: selDp1
+1: 1848024
Distance from start of the gene: 16
Sequence: ggaattcagacactctcacttatcacttcacggaatgagggttaccatacccggattgcaTtgacaggagatgtccatgag
Note(s): Zaslaver et al. demonstrated in 2006, by means of a library of fluorescent transcription fusions, that this promoter can be transcribed in vitro Zaslaver A,2006. Based on this, a putative promoter was suggested, but the +1 site of the transcription initiation has not been determined, although there exists promoter activity.
Evidence: [IEP]
[TIM]
Reference(s): [1] Sawers G., et al., 1991
[2] Zaslaver A., et al., 2006
Terminator(s)
Type: rho-independent
Sequence: aaaaaatatcCAACGAATTTTCTTGATCCGCTGgatatttcag
Reference(s): [3] DiGate RJ., et al., 1989


Transcription unit       
Name: selD-topB
Gene(s): topB, selD   Genome Browser M3D Gene expression COLOMBOS
Evidence: [BTEI] Boundaries of transcription experimentally identified
[LTED] Length of transcript experimentally determined
Reference(s): [1] Sawers G., et al., 1991
Promoter
Name: selDp2
+1: 1848029
Distance from start of the gene: 21
Sequence: gcgcaggaattcagacactctcacttatcacttcacggaatgagggttaccatacccggaTtgcattgacaggagatgtcc
Evidence: [TIM]
Reference(s): [1] Sawers G., et al., 1991
Terminator(s)
Type: rho-independent
Sequence: atatccaacgAATTTTCTTGATCCGCTGGATATttcagaatat
Reference(s): [3] DiGate RJ., et al., 1989


Transcription unit       
Name: ydjA-selD-topB
Gene(s): topB, selD, ydjA   Genome Browser M3D Gene expression COLOMBOS
Evidence: [BTEI] Boundaries of transcription experimentally identified
[LTED] Length of transcript experimentally determined
Reference(s): [1] Sawers G., et al., 1991
Promoter
Name: ydjAp
+1: 1848700
Distance from start of the gene: 24
Sequence: tcagcaggaattgcggactgtgctgtaacaaaatccactctcgtgttaattttgtgaataAatatcacgacaggagttaat
Evidence: [TIM]
Reference(s): [1] Sawers G., et al., 1991
Terminator(s)
Type: rho-independent
Sequence: aaaaaatatcCAACGAATTTTCTTGATCCGCTGgatatttcag
Reference(s): [3] DiGate RJ., et al., 1989


Evidence    

 [IEP] Inferred from expression pattern

 [TIM] Transcription initiation mapping


Reference(s)    

 [1] Sawers G., Heider J., Zehelein E., Bock A., 1991, Expression and operon structure of the sel genes of Escherichia coli and identification of a third selenium-containing formate dehydrogenase isoenzyme., J Bacteriol 173(16):4983-93

 [2] Zaslaver A., Bren A., Ronen M., Itzkovitz S., Kikoin I., Shavit S., Liebermeister W., Surette MG., Alon U., 2006, A comprehensive library of fluorescent transcriptional reporters for Escherichia coli., Nat Methods 3(8):623-8

 [3] DiGate RJ., Marians KJ., 1989, Molecular cloning and DNA sequence analysis of Escherichia coli topB, the gene encoding topoisomerase III., J Biol Chem 264(30):17924-30

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