RegulonDB RegulonDB 10.7: Operon Form
   

dsdXA operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: dsdXA
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: dsdXA
Synonym(s): OP00199, dsd
Gene(s): dsdX, dsdA   Genome Browser M3D Gene expression COLOMBOS
Note(s): dsdXA, among other genes involved in carbon source transport and metabolism, were downregulated in two MG1655 lysogens carrying closely related Stx2a phages O104 and PA8 |CITS: 31208335|.
Reference(s): [1] Norregaard-Madsen M., et al., 1995
Promoter
Name: dsdXp
+1: 2477821
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 26
Sequence: catgtgaaaggcaaattttatcgtttgtcagcctgcgttgtttttttgtccaatatcatcAggttaatcacaggggaaggt
                                 -35                  -10   +1                   
Note(s): dsdX is preceded by a putative σ70 -10 box (-7 to -12) with the sequence TCCAAT as well as a putativeσ70 -35 box (-31 to --6). The dsdXA promoter belongs to the more complex class III CRP-dependent promoters.
Evidence: [TIM] ; Method: Primer Extension
[TIM]
Reference(s): [1] Norregaard-Madsen M., et al., 1995
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote CRP-cAMP1 activator dsdXp 2477693 2477714 -117.5 tgagatgagcTAAAGTGAACCATATCTCAATTcaccttcatt nd [BCE] [1]
proximal CRP-cAMP2 activator dsdXp 2477723 2477744 -87.5 ttcaccttcaTTTTTAGATGTAAATCACTCCAttgatgcaat nd [BCE] [1]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd DsdC-D-serine1 activator dsdXp nd nd nd nd nd [GEA] [1], [2]
Note(s): 1The positive effect of the cAMP-CRP complex is seen only together with DsdC. Thus, CRP cannot act on its own in this system.
2The positive effect of the cAMP-CRP complex is seen only together with DsdC. Thus, CRP cannot act by itself in this system.1The DsdC activator can act independently and is absolutely required for the transcription activation of the dsdXA promoter.
Expression from this promoter can be futher enhanced (about seven fold) in the presence of the cAMP-CRP complex, leading to a more than 200-fold activation of transcription.
1The positive effect of the cAMP-CRP complex is seen only together with DsdC. Thus, CRP cannot act on its own in this system.
2The positive effect of the cAMP-CRP complex is seen only together with DsdC. Thus, CRP cannot act by itself in this system.
3The DsdC activator can act independently and is absolutely required for the transcription activation of the dsdXA promoter.
Expression from this promoter can be futher enhanced (about seven fold) in the presence of the cAMP-CRP complex, leading to a more than 200-fold activation of transcription.


Transcription unit          
Name: dsdA
Gene(s): dsdA   Genome Browser M3D Gene expression COLOMBOS
Evidence: [BTEI] Boundaries of transcription experimentally identified
Reference(s): [3] Bornstein-Forst SM., et al., 1987
Promoter
Name: dsdAp
+1: 2479121
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 81
Sequence: ctggctagtgaagcaatattgcggcgctacgctcaatgaaacatttaaatactatacgacAgcgacatttatcgcttcagt
                           -35                    -10       +1                   
Evidence: [TIM]
Reference(s): [3] Bornstein-Forst SM., et al., 1987
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd CRP-cAMP activator dsdAp nd nd nd nd nd [APIORCISFBSCS] [4]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd DsdC-D-serine activator dsdAp nd nd nd nd nd [GEA] [1]
External database links:
UNIPROT:
P46068





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