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| Operon |  |
|---|---|
| Name: | dsdXA |
| This page displays every known transcription unit of this operon and their known regulation. | |
| Name: | dsdXA |
| Synonym(s): | OP00199, dsd |
| Gene(s): | dsdX, dsdA Genome Browser M3D Gene expression COLOMBOS |
| Note(s): | dsdXA, among other genes involved in carbon source transport and metabolism, were downregulated in two MG1655 lysogens carrying closely related Stx2a phages O104 and PA8 1208335|. |
| Reference(s): | [1] Norregaard-Madsen M., et al., 1995 |
| Promoter | |
| Name: | dsdXp |
| +1: | 2477821 |
| Sigma Factor: | Sigma70 Sigmulon |
| Distance from start of the gene: | 26 |
| Sequence: |
catgtgaaaggcaaattttatcgtttgtcagcctgcgttgtttttttgtccaatatcatcAggttaatcacaggggaaggt -35 -10 +1 |
| Note(s): | dsdX is preceded by a putative σ70 -10 box (-7 to -12) with the sequence TCCAAT as well as a putativeσ70 -35 box (-31 to --6). The dsdXA promoter belongs to the more complex class III CRP-dependent promoters. |
| Evidence: | [TIM] |
| Reference(s): | [1] Norregaard-Madsen M., et al., 1995 |
| Type | Transcription factor | Function | Promoter | Binding Sites | Growth Conditions | Evidence (Confirmed, Strong, Weak) | Reference(s) | |||
|---|---|---|---|---|---|---|---|---|---|---|
| LeftPos | RightPos | Central Rel-Pos | Sequence | |||||||
| remote | CRP-cAMP1 | activator | dsdXp | 2477693 | 2477714 | -117.5 | tgagatgagcTAAAGTGAACCATATCTCAATTcaccttcatt | nd | [BCE] | [1] |
| proximal | CRP-cAMP2 | activator | dsdXp | 2477723 | 2477744 | -87.5 | ttcaccttcaTTTTTAGATGTAAATCACTCCAttgatgcaat | nd | [BCE] | [1] |
| Note(s): |
1The positive effect of the cAMP-CRP complex is seen only together with DsdC. Thus, CRP cannot act on its own in this system. 2The positive effect of the cAMP-CRP complex is seen only together with DsdC. Thus, CRP cannot act by itself in this system.1The DsdC activator can act independently and is absolutely required for the transcription activation of the dsdXA promoter. Expression from this promoter can be futher enhanced (about seven fold) in the presence of the cAMP-CRP complex, leading to a more than 200-fold activation of transcription. 1The positive effect of the cAMP-CRP complex is seen only together with DsdC. Thus, CRP cannot act on its own in this system. 2The positive effect of the cAMP-CRP complex is seen only together with DsdC. Thus, CRP cannot act by itself in this system. 3The DsdC activator can act independently and is absolutely required for the transcription activation of the dsdXA promoter. Expression from this promoter can be futher enhanced (about seven fold) in the presence of the cAMP-CRP complex, leading to a more than 200-fold activation of transcription. |
| Name: | dsdA |
| Gene(s): | dsdA Genome Browser M3D Gene expression COLOMBOS |
| Note(s): | A potential RNA G-quadruplex structure, formed by guanine-rich sequences located in the coding sequence region of the gene, was identified for dsdA . This structure could regulate the expression of the gene, as observed for hemL gene expression 31964733. |
| Evidence: | [BTEI] Boundaries of transcription experimentally identified |
| Reference(s): | [3] Bornstein-Forst SM., et al., 1987 |
| Promoter | |
| Name: | dsdAp |
| +1: | 2479121 |
| Sigma Factor: | Sigma70 Sigmulon |
| Distance from start of the gene: | 81 |
| Sequence: |
ctggctagtgaagcaatattgcggcgctacgctcaatgaaacatttaaatactatacgacAgcgacatttatcgcttcagt -35 -10 +1 |
| Evidence: | [TIM] |
| Reference(s): | [3] Bornstein-Forst SM., et al., 1987 |
[TIM] Transcription initiation mapping