RegulonDB RegulonDB 10.8: Operon Form
   

bolA operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: bolA
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit       
Name: bolA
Gene(s): bolA   Genome Browser M3D Gene expression COLOMBOS
Evidence: [BTEI] Boundaries of transcription experimentally identified
Reference(s): [1] Aldea M., et al., 1989
Promoter
Name: bolAp2
+1: 454349
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 123
Sequence: ctggtaaaagaaacactgatgcgaggcctgtgtttcaatctttaaatcagtaaacttcatAcgcttgacggaaaaaccagg
                              -35                  -10      +1                   
Evidence: [AIPP]
[TIM]
Reference(s): [1] Aldea M., et al., 1989
Terminator(s)
Type: rho-independent
Sequence: gattttatgaAAAACGGCCTGCGGGCCGTTTTgttttgtctg
Reference(s): [1] Aldea M., et al., 1989


Transcription unit          
Name: bolA
Gene(s): bolA   Genome Browser M3D Gene expression COLOMBOS
Note(s): bolA gene expression levels are higher in biofilms than in exponential-phase planktonic cells 21219458 bolA facilitates biofilm development through σS 21219458
The bolA and rpoS genes are expressed in response to sudden changes in the environment 21219458
Evidence: [BTEI] Boundaries of transcription experimentally identified
Reference(s): [1] Aldea M., et al., 1989
[2] Bohannon DE., et al., 1991
Promoter
Name: bolAp1
+1: 454434
Sigma Factor: Sigma38, Sigma70
Distance from start of the gene: 38
Sequence: aacctaaatatttgttgttaagctgcaatggaaacggtaaaagcggctagtatttaaaggGatggatgacatctcagcgtt
                       -35                 -10              +1                   
Evidence: [AIPP]
[CV(RPF)]
[CV(RPF/RS-EPT-CBR)]
[CV(RPF/TIM)]
[CV(RS-EPT-CBR)]
[CV(TIM)]
[RPF]
[RS-EPT-CBR]
[TIM]
Reference(s): [1] Aldea M., et al., 1989
[2] Bohannon DE., et al., 1991
[3] Kvint K., et al., 2000
[4] Nguyen LH., et al., 1997
[5] Salgado H, et al., 2012
[6] Yamamoto K., et al., 2000
Terminator(s)
Type: rho-independent
Sequence: gattttatgaAAAACGGCCTGCGGGCCGTTTTgttttgtctg
Reference(s): [1] Aldea M., et al., 1989
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd H-NS repressor bolAp1 nd nd nd nd nd [GEA] [7]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal OmpR-P repressor bolAp1 454377 454396 -47.5 aggacgaaacCTAAATATTTGTTGTTAAGCtgcaatggaa nd [APIORCISFBSCS], [BPP] [6]
Allosteric regulation of RNA-polymerase
  Regulator Function Promoter target of RNApol Growth Conditions Note Evidence Reference
  ppGpp activation bolAp1   [GEA]
[IEP]
[3]
[8]
Evidence: [GEA] Gene expression analysis
[IEP] Inferred from expression pattern
Reference(s): [3] Kvint K., et al., 2000
[8] Traxler MF., et al., 2008




Reference(s)    

 [1] Aldea M., Garrido T., Hernandez-Chico C., Vicente M., Kushner SR., 1989, Induction of a growth-phase-dependent promoter triggers transcription of bolA, an Escherichia coli morphogene., EMBO J 8(12):3923-31

 [2] Bohannon DE., Connell N., Keener J., Tormo A., Espinosa-Urgel M., Zambrano MM., Kolter R., 1991, Stationary-phase-inducible "gearbox" promoters: differential effects of katF mutations and role of sigma 70., J Bacteriol 173(14):4482-92

 [3] Kvint K., Farewell A., Nystrom T., 2000, RpoS-dependent promoters require guanosine tetraphosphate for induction even in the presence of high levels of sigma(s)., J Biol Chem 275(20):14795-8

 [4] Nguyen LH., Burgess RR., 1997, Comparative analysis of the interactions of Escherichia coli sigma S and sigma 70 RNA polymerase holoenzyme with the stationary-phase-specific bolAp1 promoter., Biochemistry 36(7):1748-54

 [5] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [6] Yamamoto K., Nagura R., Tanabe H., Fujita N., Ishihama A., Utsumi R., 2000, Negative regulation of the bolA1p of Escherichia coli K-12 by the transcription factor OmpR for osmolarity response genes., FEMS Microbiol Lett 186(2):257-62

 [7] Barth M., Marschall C., Muffler A., Fischer D., Hengge-Aronis R., 1995, Role for the histone-like protein H-NS in growth phase-dependent and osmotic regulation of sigma S and many sigma S-dependent genes in Escherichia coli., J Bacteriol 177(12):3455-64

 [8] Traxler MF., Summers SM., Nguyen HT., Zacharia VM., Hightower GA., Smith JT., Conway T., 2008, The global, ppGpp-mediated stringent response to amino acid starvation in Escherichia coli., Mol Microbiol 68(5):1128-48


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