RegulonDB RegulonDB 10.7: Operon Form
   

osmB operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: osmB
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit       
Name: osmB
Gene(s): osmB   Genome Browser M3D Gene expression COLOMBOS
Note(s): The expression of the gene osmB is increased when colicin (at 30 ng/ml) is added to the medium |CITS: [23421615]|.
Evidence: [BTEI] Boundaries of transcription experimentally identified
Reference(s): [1] Jung JU., et al., 1989
Promoter
Name: osmBp2
+1: 1343369
Sigma Factor: Sigma70, Sigma38
Distance from start of the gene: 41
Sequence: gcgcgagcagatttcacggaataatttcaccagacttattcttagctattatagttatagAgagcttacttccgtgaatca
                          -35                  -10          +1                   
Note(s): The osmBp2 promoter is activated by potassium glutamate but only when σS is used. σ70 recognizes osmBp2 at low osmolarity. Only osmBp2 is induced by stationary phase at low osmolarity. The two promoters are active when both high osmolarity and stationary-phase signals are present simultaneously, but the osmBp2 activity is much greater than the osmBp1 activity.
osmBp2 was unambiguously characterized by Jung JU,1989 Boulanger A,2005.
Evidence: [HIPP]
[TIM]
Reference(s): [2] Boulanger A., et al., 2005
[3] Ding Q., et al., 1995
[4] Jung JU., et al., 1990
[1] Jung JU., et al., 1989


Transcription unit          
Name: osmB
Gene(s): osmB   Genome Browser M3D Gene expression COLOMBOS
Note(s): The expression of the gene osmB is increased when colicin (at 30 ng/ml) is added to the medium |CITS: [23421615]|.
Evidence: [BTEI] Boundaries of transcription experimentally identified
Reference(s): [1] Jung JU., et al., 1989
Promoter
Name: osmBp1
+1: 1343476
Sigma Factor: Sigma38 Sigmulon
Distance from start of the gene: 148
Sequence: ctggttgataattaagactatttacctgttattaacactctcaagatataaaattattatCagcgatataacaggaagtca
                                -35               -10       +1                   
Note(s): Only osmBp2 is induced during stationary phase at low osmolarity. The two promoters are active when both high osmolarity and stationary phase signals are present simultaneously, but the osmBp2 activity is much greater than the osmBp1 activity.
The transcriptional start site of the osmBp1 promoter determined by Jung JU,1989 is different by 1 bp from the one characterized by Boulanger A,2005
Evidence: [TIM]
Reference(s): [2] Boulanger A., et al., 2005
[4] Jung JU., et al., 1990
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal RcsB-Pasp56 activator osmBp1 1343511 1343524 -41.5 ggttgataatTAAGACTATTTACCtgttattaac nd [APIORCISFBSCS], [GEA] [2]


Regulation by sRNA    
  Small RNA name (Regulator) Regulation type Mechanism Function Binding Sites Evidence Reference
LeftPos RightPos Sequence (RNA-strand)
  omrA antisense post-transcriptional regulation repressor       [IMP] [5]
[6]
Notes: "The provided sequence is that of the RNA strand,i.e. 'U's are showed instead the 'T'"





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