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|
| Operon |  |
|---|---|
| Name: | otsBA |
| This page displays every known transcription unit of this operon and their known regulation. | |
| Name: | otsA |
| Gene(s): | otsA Genome Browser M3D Gene expression COLOMBOS |
| Note(s): | The start codon of otsA is located 23 nt before the end of otsB, resulting in an overlap between the ots genes. |
| Evidence: | [PM] Polar mutation |
| Reference(s): | [1] Kaasen I., et al., 1992 |
| Promoter | |
| Name: | otsAp |
| +1: | 1981651 |
| Sigma Factor: | Sigma38 Sigmulon |
| Distance from start of the gene: | 39 |
| Sequence: |
catcatggcgactggcgggtgtgccggatgtctggagctggcttgaaatgataaccaccgCattacaacaaaaaagagaaa -35 -10 +1 |
| Note(s): | The otsA promoter is an internal weak promoter, and it is within the otsB gene and 39 nt upstream from the otsA start codon. Upstream of otsB there is an inverted repeat, and a half-site of this repeat is also found in front of otsA. These may represent binding sites for a regulatory protein. The transcription of the otsBA operon is reduced when the rpoS gene is inactivated, but whether there is a σS-type interaction (direct or indirect) with the otsBA operon is not known. |
| Evidence: |
[HIPP] [TIM] |
| Reference(s): |
[2] Hengge-Aronis R., et al., 1991 [3] Kaasen I., et al., 1994 |
| Terminator(s) | |
| Type: | rho-independent |
| Sequence: | aatcacagttTCTTTTCGCGAGCATCCTGCTCGGCGAAAAGagttattgcc |
| Reference(s): | [3] Kaasen I., et al., 1994 |
| Name: | otsBA |
| Gene(s): | otsA, otsB Genome Browser M3D Gene expression COLOMBOS |
| Note(s): | The start codon of otsA is located 23 nt before the end of otsB, resulting in an overlap between the ots genes. |
| Evidence: | [PM] Polar mutation |
| Reference(s): | [1] Kaasen I., et al., 1992 |
| Promoter | |
| Name: | otsBp2 |
| +1: | 1982442 |
| Sigma Factor: | Sigma38 Sigmulon |
| Distance from start of the gene: | 55 |
| Sequence: |
taacggttggctgttcttcgttgcaaatggcgacccccgtcacactgtctatacttacatGtctgtaaagcgcgttctgcg -35 -10 +1 |
| Note(s): | The operon otsAB is positively regulated by RpoS in stationary phase and is mainly controlled at the transcription level Hengge-Aronis R,1991. Strøm AR, Kaasen I,1993 It is also osmotically regulated. Region 4 of the σ38 protein and the elements near the -35 DNA region are involved in triggering the response to high salt concentrations, which ensures the transcriptional activation of the otsBp2 promoter to counteract this physiological challenge Rosenthal AZ,2006. |
| Evidence: |
[RS-EPT-CBR] [TIM] |
| Reference(s): |
[4] Becker G., et al., 2001 [3] Kaasen I., et al., 1994 [5] Rosenthal AZ., et al., 2006 [6] Salgado H, et al., 2012 |
| Terminator(s) | |
| Type: | rho-independent |
| Sequence: | aatcacagttTCTTTTCGCGAGCATCCTGCTCGGCGAAAAGagttattgcc |
| Reference(s): | [3] Kaasen I., et al., 1994 |
| Name: | otsBA |
| Gene(s): | otsA, otsB Genome Browser M3D Gene expression COLOMBOS |
| Note(s): | The start codon of otsA is located 23 nt before the end of otsB, resulting in an overlap between the ots genes. |
| Evidence: | [PM] Polar mutation |
| Reference(s): | [1] Kaasen I., et al., 1992 |
| Promoter | |
| Name: | otsBp |
| +1: | 1982465 |
| Sigma Factor: | Sigma38 Sigmulon |
| Distance from start of the gene: | 78 |
| Sequence: |
tttcccaccatagccaaccgccataacggttggctgttcttcgttgcaaatggcgaccccCgtcacactgtctatacttac -35 -10 +1 |
| Note(s): | Upstream of otsB there is an inverted repeat, and a half-site of this repeat is also found in front of otsA. These may represent binding sites for a regulatory protein. The transcription of the otsBA operon is reduced when the rpoS gene is inactivated, but it is not clear whether the σS effect is the result of a direct or indirect interaction on the otsBA operon. |
| Evidence: |
[HIPP] [TIM] |
| Reference(s): |
[2] Hengge-Aronis R., et al., 1991 [3] Kaasen I., et al., 1994 |
| Terminator(s) | |
| Type: | rho-independent |
| Sequence: | aatcacagttTCTTTTCGCGAGCATCCTGCTCGGCGAAAAGagttattgcc |
| Reference(s): | [3] Kaasen I., et al., 1994 |
| Reference(s) |
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|---|---|
[HIPP] Human inference of promoter position