RegulonDB RegulonDB 10.8: Operon Form
   

fepB operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: fepB
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: fepB
Synonym(s): OP00225
Gene(s): fepB   Genome Browser M3D Gene expression COLOMBOS
Reference(s): [1] Elkins MF., et al., 1989
Promoter
Name: fepBp
+1: 624727
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 217
Sequence: ggtaacgcaatctgcaactcggggttgcatctggttgtggttacaatgaaaatgagaagcAttattgatggattcgcataa
                         -35                    -10         +1                   
Evidence: [HIPP]
[IMP]
[TIM]
Reference(s): [2] Brickman TJ., et al., 1990
TF binding sites (TFBSs)
[Fur,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal Fur-Fe2+ repressor fepBp 624719 624737 -1.0 ttacaatgaaAATGAGAAGCATTATTGATggattcgcat nd [AIBSCS], [APIORCISFBSCS], [BPP], [CV(GEA)], [CV(GEA)], [GEA] [2], [3], [4], [5]
proximal Fur-Fe2+ repressor fepBp 624725 624743 -7.0 ttgtggttacAATGAAAATGAGAAGCATTattgatggat nd [AIBSCS], [APIORCISFBSCS], [BPP], [CV(GEA)], [CV(GEA)], [CV(GEA/SM)], [CV(SM)], [GEA], [SM] [2], [3], [4], [5]
proximal Fur-Fe2+ repressor fepBp 624731 624749 -13.0 atctggttgtGGTTACAATGAAAATGAGAagcattattg nd [APIORCISFBSCS], [BPP] [2]
[RutR,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote RutR repressor fepBp 624548 624564 172.5 gattgtgcgcTTTGTCGAATTTGTCATtacgccctta nd [AIBSCS], [BPP], [CV(GEA)], [CV(GEA)], [GEA] [6]


Evidence    

 [HIPP] Human inference of promoter position

 [IMP] Inferred from mutant phenotype

 [TIM] Transcription initiation mapping

 [AIBSCS] Automated inference based on similarity to consensus sequences

 [APIORCISFBSCS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.

 [BPP] Binding of purified proteins

 [CV(GEA)] cross validation(GEA)

 [GEA] Gene expression analysis

 [CV(GEA/SM)] cross validation(GEA/SM)

 [CV(SM)] cross validation(SM)

 [SM] Site mutation


Reference(s)    

 [1] Elkins MF., Earhart CF., 1989, Nucleotide sequence and regulation of the Escherichia coli gene for ferrienterobactin transport protein FepB., J Bacteriol 171(10):5443-51

 [2] Brickman TJ., Ozenberger BA., McIntosh MA., 1990, Regulation of divergent transcription from the iron-responsive fepB-entC promoter-operator regions in Escherichia coli., J Mol Biol 212(4):669-82

 [3] Chen Z., Lewis KA., Shultzaberger RK., Lyakhov IG., Zheng M., Doan B., Storz G., Schneider TD., 2007, Discovery of Fur binding site clusters in Escherichia coli by information theory models., Nucleic Acids Res 35(20):6762-77

 [4] Newman DL., Shapiro JA., 1999, Differential fiu-lacZ fusion regulation linked to Escherichia coli colony development., Mol Microbiol 33(1):18-32

 [5] Vassinova N., Kozyrev D., 2000, A method for direct cloning of fur-regulated genes: identification of seven new fur-regulated loci in Escherichia coli., Microbiology 146 Pt 12:3171-3182

 [6] Shimada T., Ishihama A., Busby SJ., Grainger DC., 2008, The Escherichia coli RutR transcription factor binds at targets within genes as well as intergenic regions., Nucleic Acids Res 36(12):3950-5

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