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Name: | gcvTHP |
Synonym(s): | OP00232, gcv |
Gene(s): | gcvP, gcvH, gcvT Genome Browser M3D Gene expression COLOMBOS |
Note(s): | The gcvTHP operon is expressed at a higher level in LB medium than in minimal medium. Glycine enhances its expression under minimal medium conditions Yang D,2020. Based on gene expression analysis, it was shown that CsgD could positively regulate gcvTHP expression Herrington MB,2013 |
Reference(s): |
[1] Okamura-Ikeda K., et al., 1993 [2] Stauffer LT., et al., 1994 |
Promoter | |
Name: | gcvTp |
+1: | 3050772 |
Sigma Factor: | Sigma70 Sigmulon |
Distance from start of the gene: | 105 |
Sequence: |
atgtgatgatcaattttaccttatggttaacagtctgtttcggtggtaagttcaggcaaaAgagaacgattgcgttgggga -35 -10 +1 |
Note(s): | gcvTp is a class III activator-dependent promoter (where multiple activator molecules typically bind more than 90 bp upstream of the RNA polymerase-binding site) Stauffer LT,2005 |
Evidence: | [TIM] |
Reference(s): |
[1] Okamura-Ikeda K., et al., 1993 [3] Stauffer LT., et al., 1993 [4] Stauffer LT., et al., 1994 |
Terminator(s) | |
Type: | rho-independent |
Sequence: | atctgactaaAAAGGCGCCGAAGCGCCTTTagaaaatagt |
Reference(s): | [2] Stauffer LT., et al., 1994 |
Type | Transcription factor | Function | Promoter | Binding Sites | Growth Conditions | Evidence (Confirmed, Strong, Weak) | Reference(s) | |||
---|---|---|---|---|---|---|---|---|---|---|
LeftPos | RightPos | Central Rel-Pos | Sequence | |||||||
remote | CRP-cAMP | activator | gcvTp | 3050907 | 3050929 | -145.5 | tgtcttattcAAATTGACTTTCTCATCACATCAtctttgtata | nd | [APIORCISFBSCS], [BPP], [CV(GEA)], [CV(GEA)], [CV(GEA/SM)], [CV(SM)], [GEA], [SM] | [5] |
remote | CRP-cAMP | activator | gcvTp | 3051075 | 3051097 | -313.5 | tcctctgttgTTTATTTGATACCCATCACACTTtcatctcccg | nd | [APIORCISFBSCS], [BPP], [CV(GEA)], [CV(GEA)], [CV(GEA/SM)], [CV(SM)], [GEA], [SM] | [5] |
Type | Transcription factor | Function | Promoter | Binding Sites | Growth Conditions | Evidence (Confirmed, Strong, Weak) | Reference(s) | |||
---|---|---|---|---|---|---|---|---|---|---|
LeftPos | RightPos | Central Rel-Pos | Sequence | |||||||
remote | GcvA1 | activator | gcvTp | 3050991 | 3051005 | -226.0 | ccgttttcgcATTATATTTTCTAATgccattattt | nd | [APIORCISFBSCS], [BCE], [BPP], [CV(GEA)], [CV(GEA)], [CV(GEA/SM)], [CV(SM)], [GEA], [SM] | [6], [7], [8], [9], [10], [11], [12], [13] |
remote | GcvA2 | activator | gcvTp | 3051023 | 3051037 | -258.0 | ttttcctcatTTGAAATAAACTAATttcacctccg | nd | [APIORCISFBSCS], [BCE], [BPP], [CV(GEA)], [CV(GEA)], [CV(GEA/SM)], [CV(SM)], [GEA], [SM] | [6], [7], [8], [9], [10], [11], [12], [13] |
Type | Transcription factor | Function | Promoter | Binding Sites | Growth Conditions | Evidence (Confirmed, Strong, Weak) | Reference(s) | |||
---|---|---|---|---|---|---|---|---|---|---|
LeftPos | RightPos | Central Rel-Pos | Sequence | |||||||
proximal | GcvA | repressor | gcvTp | 3050809 | 3050823 | -44.0 | aatgtgatgaTCAATTTTACCTTATggttaacagt | nd | [APIORCISFBSCS], [BCE], [BPP], [CV(GEA)], [CV(GEA)], [CV(GEA/SM)], [CV(SM)], [GEA], [IC], [SM] | [6], [8], [11], [13], [15], [16] |
proximal | GcvA | repressor | gcvTp | 3050831 | 3050845 | -66.0 | ttgcatttttTAACCATAAGCTAATgtgatgatca | nd | [APIORCISFBSCS], [BCE], [BPP], [CV(GEA)], [CV(GEA)], [CV(GEA/SM)], [CV(SM)], [GEA], [SM] | [6], [8], [11], [13], [15], [16] |
remote | GcvA3 | repressor | gcvTp | 3050991 | 3051005 | -226.0 | ccgttttcgcATTATATTTTCTAATgccattattt | nd | [APIORCISFBSCS], [BCE], [BPP], [CV(GEA)], [CV(GEA)], [CV(GEA/SM)], [CV(SM)], [GEA], [SM] | [6], [7], [8], [9], [10], [11], [12], [13] |
remote | GcvA4 | repressor | gcvTp | 3051023 | 3051037 | -258.0 | ttttcctcatTTGAAATAAACTAATttcacctccg | nd | [APIORCISFBSCS], [BCE], [BPP], [CV(GEA)], [CV(GEA)], [CV(GEA/SM)], [CV(SM)], [GEA], [SM] | [6], [7], [8], [9], [10], [11], [12], [13] |
Type | Transcription factor | Function | Promoter | Binding Sites | Growth Conditions | Evidence (Confirmed, Strong, Weak) | Reference(s) | |||
---|---|---|---|---|---|---|---|---|---|---|
LeftPos | RightPos | Central Rel-Pos | Sequence | |||||||
proximal | PurR-hypoxanthine | repressor | gcvTp | 3050758 | 3050773 | 7.5 | gttcaggcaaAAGAGAACGATTGCGTtggggaccgg | nd | [AIBSCS], [APIORCISFBSCS], [CV(GEA)], [GEA] | [13], [17] |
Note(s): |
1By making use of microarray analyses it was concluded that anarobiosis does not affect expression of the gcv operon, although FNR appears to activate it. A putative FNR-binding site, which was not shown in the report, was identified upstream of this operon Salmon K,2003.1GcvA interacts with the RNA polymerase α-subunit to activate gcvTp Stauffer LT,2005 2GcvA interacts with the RNA polymerase α-subunit to activate gcvTp Stauffer LT,20053GcvA interacts with the RNA polymerase α-subunit to activate gcvTp Stauffer LT,2005 4GcvA interacts with the RNA polymerase α-subunit to activate gcvTp Stauffer LT,20051Lrp takes part in gcv operon regulation as a modulator of the GcvA effect. It bends the DNA upstream of the transcription start position to allow GcvA to function Stauffer LT,19992GcvA interacts with the RNA polymerase α-subunit to activate gcvTp Stauffer LT,2005 3GcvA interacts with the RNA polymerase α-subunit to activate gcvTp Stauffer LT,2005 4GcvA interacts with the RNA polymerase α-subunit to activate gcvTp Stauffer LT,2005 5GcvA interacts with the RNA polymerase α-subunit to activate gcvTp Stauffer LT,2005 6Lrp takes part in gcv operon regulation as a modulator of the GcvA effect. It bends the DNA upstream of the transcription start position to allow GcvA to function Stauffer LT,1999 11By making use of microarray analyses it was concluded that anarobiosis does not affect expression of the gcv operon, although FNR appears to activate it. A putative FNR-binding site, which was not shown in the report, was identified upstream of this operon Salmon K,2003. |
Name: | gcvP |
Gene(s): | gcvP Genome Browser M3D Gene expression COLOMBOS |
Evidence: | [BTEI] Boundaries of transcription experimentally identified |
Reference(s): | [19] Zaslaver A., et al., 2006 |
Promoter | |
Name: | gcvPp |
+1: | Unknown |
Evidence: | [IEP] |
Reference(s): | [19] Zaslaver A., et al., 2006 |
Terminator(s) | |
Type: | rho-independent |
Sequence: | atctgactaaAAAGGCGCCGAAGCGCCTTTagaaaatagt |
Reference(s): | [2] Stauffer LT., et al., 1994 |
Reference(s) |
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