RegulonDB RegulonDB 10.6.3: Operon Form
   

glpFKX operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: glpFKX
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: glpFKX
Synonym(s): OP00237
Gene(s): glpX, glpK, glpF   Genome Browser M3D Gene expression COLOMBOS
Note(s): The first documented case of transposon-mediated directed mutagenesis has been determined for the glpFKX promoter region, where CRP acts as activator and GlpR as repressor |CITS:[22248543]|.
Evidence: [BTEI] Boundaries of transcription experimentally identified
[PM] Polar mutation
Reference(s): [1] Lupski JR., et al., 1990
[2] Truniger V., et al., 1992
[3] Weissenborn DL., et al., 1992
Promoter
Name: glpFp
+1: 4118161
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 71
Sequence: ggcacacacattttaagttcgatatttctcgtttttgctcgttaacgataagtttacagcAtgcctacaagcatcgtggag
                          -10                    -35        +1                   
Evidence: [HIPP]
[TIM] ; Method: Primer Extension
[TIM]
Reference(s): [3] Weissenborn DL., et al., 1992
Terminator(s)
Type: rho-independent
Sequence: aacaaaaaaaTTCCAGTCCCGAAGGACTGGAAggctcaatcg
Reference(s): [2] Truniger V., et al., 1992
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal CRP-cAMP activator glpFp 4118188 4118209 -37.5 cacacacattTTAAGTTCGATATTTCTCGTTTttgctcgtta nd [GEA], [HIBSCS] [3]
proximal CRP-cAMP activator glpFp 4118211 4118232 -60.5 ataacgctcaTTTTATGACGAGGCACACACATtttaagttcg nd [GEA], [HIBSCS] [3]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote GlpR1 repressor glpFp 4117109 4117128 1043.5 attagcgtgtCGCAGCGCGAATTTGAGCAAatctacccaa nd [BPP], [HIBSCS] [3]
remote GlpR2 repressor glpFp 4117151 4117170 1001.5 accagctcccGCGCGGTCGTAATGGATCACgatgccaata nd [BPP], [HIBSCS] [3]
proximal GlpR3 repressor glpFp 4118169 4118188 -17.5 atttctcgttTTTGCTCGTTAACGATAAGTttacagcatg nd [BPP], [HIBSCS] [3]
proximal GlpR4 repressor glpFp 4118189 4118208 -37.5 acacacatttTAAGTTCGATATTTCTCGTTtttgctcgtt nd [BPP], [HIBSCS] [3]
proximal GlpR5 repressor glpFp 4118210 4118229 -58.5 acgctcatttTATGACGAGGCACACACATTttaagttcga nd [BPP], [HIBSCS] [3]
proximal GlpR6 repressor glpFp 4118231 4118250 -79.5 gatggaataaATGGCGCGATAACGCTCATTttatgacgag nd [BPP], [HIBSCS] [3]
Note(s): 1This binding site is within the glpK coding region. The affinity of the repressor for this site is much lower than the affinity of it for the sites in the regulatory region.
2This binding site is within the glpK coding region. The affinity of the repressor for this site is much lower than the affinity of it for the sites in the regulatory region.
3GlpR perhaps binds cooperatively to four adjacent operator sites.
4GlpR perhaps binds cooperatively to four adjacent operator sites.
5GlpR perhaps binds cooperatively to four adjacent operator sites.
6GlpR perhaps binds cooperatively to four adjacent operator sites.1GlpR perhaps binds cooperatively to four adjacent operator sites.
3GlpR perhaps binds cooperatively to four adjacent operator sites.
5GlpR perhaps binds cooperatively to four adjacent operator sites.
6GlpR perhaps binds cooperatively to four adjacent operator sites.
7This binding site is within the glpK coding region. The affinity of the repressor for this site is much lower than the affinity of it for the sites in the regulatory region.
8This binding site is within the glpK coding region. The affinity of the repressor for this site is much lower than the affinity of it for the sites in the regulatory region.





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