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Name: | thrLABC |
Synonym(s): | OP00248, thr, thrABC |
Gene(s): | thrL, thrA, thrB, thrC Genome Browser M3D Gene expression COLOMBOS |
Note(s): | The thrLABC operon codes for four enzymes involved in threonine and homoserine biosynthesis. thrLABC is regulated in response to the abundance of both isoleucine and threonine 13959617. Part of this regulation presumably occurs via attenuation, as thrLABC contains a leader sequence rich in threonine and isoleucine codons followed immediately by a functional transcriptional terminator that acts to prevent transcription of the enzyme-coding genes in the operon Lynn SP,1982. Gardner JF.,1979. 2410621. 1709279. |
Evidence: | [PM] Polar mutation |
Reference(s): |
[1] Lynn SP., et al., 1987 [2] Parsot C., et al., 1983 [3] Theze J., et al., 1974 |
Promoter | |
Name: | thrLp |
+1: | 148 |
Sigma Factor: | Sigma70 Sigmulon |
Distance from start of the gene: | 42 |
Sequence: |
gccgtgagtaaattaaaattttattgacttaggtcactaaatactttaaccaatataggcAtagcgcacagacagataaaa -35 -10 +1 |
Evidence: |
[CV(RS-EPT-CBR)] [CV(TIM)] [IMP] [RS-EPT-CBR] [TIM] |
Reference(s): |
[4] Gardner JF. 1982 [5] Lynn SP., et al., 1982 [6] Salgado H, et al., 2012 |
Terminator(s) | |
Type: | rho-independent |
Sequence: | atctattcatTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATtatggagaaa |
Reference(s): |
[7] Feng CQ., et al., 2019 [8] Lesnik EA., et al., 2001 |
Evidence: |
[APPH] Assay of protein purified to homogeneity [IEP] Inferred from expression pattern |
Reference(s): |
[9] Lee JH., et al., 2012 [10] Mechold U., et al., 2013 |
Name: | thrL |
Gene(s): | thrL Genome Browser M3D Gene expression COLOMBOS |
Evidence: | [BTEI] Boundaries of transcription experimentally identified |
Reference(s): |
[11] Gardner JF. 1979 [4] Gardner JF. 1982 |
Promoter | |
Name: | thrLp |
+1: | 148 |
Sigma Factor: | Sigma70 Sigmulon |
Distance from start of the gene: | 42 |
Sequence: |
gccgtgagtaaattaaaattttattgacttaggtcactaaatactttaaccaatataggcAtagcgcacagacagataaaa -35 -10 +1 |
Evidence: |
[CV(RS-EPT-CBR)] [CV(TIM)] [IMP] [RS-EPT-CBR] [TIM] |
Reference(s): |
[4] Gardner JF. 1982 [5] Lynn SP., et al., 1982 [6] Salgado H, et al., 2012 |
Terminator(s) | |
Type: | rho-independent |
Sequence: | ggaaacacagAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTcgaccaaagg |
Reference(s): | [11] Gardner JF. 1979 |
Evidence: |
[APPH] Assay of protein purified to homogeneity [IEP] Inferred from expression pattern |
Reference(s): |
[9] Lee JH., et al., 2012 [10] Mechold U., et al., 2013 |
RNA cis-regulatory element | ![]() |
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Regulation, transcriptional elongation | |
Attenuator type: | Transcriptional |
Strand: | forward |
Structure type | Energy | LeftPos | RightPos | Sequence (RNA-strand) | |
---|---|---|---|---|---|
terminator | -20.0 | 276 | 311 | aacacagaaaAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCgaccaaaggt | |
anti-terminator | -19.41 | 244 | 288 | acaggtaacgGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCtgacagtgcg | |
anti-anti-terminator | -14.5 | 200 | 255 | tgaaacgcatTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGAcgcgtacagg |
Notes: "The provided "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appear as the reverse complement of the sequence delimited by LeftPos-RigtPos" |
REGULATION, RNA cis-regulatory element: | |
Rfam type: | Cis-reg; leader |
Strand: | forward |
Description | Rfam score | Left Pos | Right Pos | Sequence (RNA-strand) | |
---|---|---|---|---|---|
Threonine operon leader | 122.9 | 191 | 310 | acaacauccaUGAAACGCAUUAGCACCACCAUUACCACCACCAUCACCAUUACCACAGGUAACGGUGCGGGCUGACGCGUACAGGAAACACAGAAAAAAGCCCGCACCUGACAGUGCGGGCUUUUUUUUUcgaccaaagg |
Notes: "The provied "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appears as the reverse complement of the sequence delimited by LeftPos-RightPos" |
Reference(s) |
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