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| Operon |  |
|---|---|
| Name: | leuLABCD |
| This page displays every known transcription unit of this operon and their known regulation. | |
| Name: | leuLABCD |
| Synonym(s): | OP00249, leu, leuABCD |
| Gene(s): | leuD, leuC, leuB, leuA, leuL Genome Browser M3D Gene expression COLOMBOS |
| Note(s): | The leuLABCD operon encodes the enzymes reponsible for the biosynthesis of leucine from valine. It contains a terminator region following leuL at which transcription terminates in vitro Wessler SR,1981. Gemmill RM,1983. This is presumed to be a component of leucine-based attenuation control of this operon, as the leader peptide contains a quartet of leucines, and insertions within the leader disrupt transcription of leuLABCD Vartak NB,1991. Lrp was previously reported to regulate this operon Lin R,1992, but a later study showed that any Lrp effect was indirect 10515950. |
| Evidence: | [PAGTSBP] Products of adjacent genes in the same biological process |
| Reference(s): | [1] Somers JM., et al., 1973 |
| Promoter | |
| Name: | leuLp |
| +1: | 83735 |
| Sigma Factor: | Sigma70 Sigmulon |
| Distance from start of the gene: | 27 |
| Sequence: |
cgggacgtttttattgcgtcagggttgacatccgtttttgtatccagtaactctaaaagcAtatcgcattcatctggagct -35 -10 +1 |
| Evidence: |
[CV(RS-EPT-CBR)] [CV(TIM)] [HIPP] [RS-EPT-CBR] [TIM] |
| Reference(s): |
[2] Gemmill RM., et al., 1983 [3] Salgado H, et al., 2012 [1] Somers JM., et al., 1973 [4] Stratmann T., et al., 2012 [5] Wessler SR., et al., 1981 |
| Evidence: |
[GEA] Gene expression analysis [IEP] Inferred from expression pattern |
| Reference(s): |
[7] Durfee T., et al., 2008 [8] Reimers JM., et al., 2004 |
| Name: | leuL |
| Gene(s): | leuL Genome Browser M3D Gene expression COLOMBOS |
| Note(s): | The mRNA that contains the leuL gene has been observed mainly in the poles of the cell 31540875. |
| Evidence: | [BTEI] Boundaries of transcription experimentally identified |
| Reference(s): |
[2] Gemmill RM., et al., 1983 [4] Stratmann T., et al., 2012 |
| Promoter | |
| Name: | leuLp |
| +1: | 83735 |
| Sigma Factor: | Sigma70 Sigmulon |
| Distance from start of the gene: | 27 |
| Sequence: |
cgggacgtttttattgcgtcagggttgacatccgtttttgtatccagtaactctaaaagcAtatcgcattcatctggagct -35 -10 +1 |
| Evidence: |
[CV(RS-EPT-CBR)] [CV(TIM)] [HIPP] [RS-EPT-CBR] [TIM] |
| Reference(s): |
[2] Gemmill RM., et al., 1983 [3] Salgado H, et al., 2012 [1] Somers JM., et al., 1973 [4] Stratmann T., et al., 2012 [5] Wessler SR., et al., 1981 |
| Terminator(s) | |
| Type: | rho-independent |
| Sequence: | ttcgggcataAAAAAACCCGCGCAATGGCGCGGGTTTTTTgtttgactgc |
| Reference(s): | [5] Wessler SR., et al., 1981 |
| Evidence: |
[GEA] Gene expression analysis [IEP] Inferred from expression pattern |
| Reference(s): |
[7] Durfee T., et al., 2008 [8] Reimers JM., et al., 2004 |
| Name: | leuLABCD |
| Gene(s): | leuD, leuC, leuB, leuA, leuL Genome Browser M3D Gene expression COLOMBOS |
| Evidence: | [BTEI] Boundaries of transcription experimentally identified |
| Reference(s): | [4] Stratmann T., et al., 2012 |
| Promoter | |
| Name: | leuLp2 |
| +1: | 84024 |
| Distance from start of the gene: | 316 |
| Sequence: |
taattaatttatatatttaatgcattaattcttaacattaattgatcaataatattcaccAaatcaatatcaaaaaaaatc |
| Evidence: | [TIM] |
| Reference(s): | [4] Stratmann T., et al., 2012 |
| RNA cis-regulatory element | |
|---|
| Regulation, transcriptional elongation | |
| Attenuator type: | Transcriptional |
| Strand: | reverse |
| Structure type | Energy | LeftPos | RightPos | Sequence (RNA-strand) | |
|---|---|---|---|---|---|
| terminator | -16.9 | 83571 | 83600 | gtcaaacaaaAAACCCGCGCCATTGCGCGGGTTTTTTTAtgcccgaagc | |
| anti-terminator | -19.72 | 83591 | 83655 | catcttctttGCGCGGTAGACGAGTGAGCGGCATCCAGCATTAAGCCAGCACGCAGTCAAACAAAAAACCCGCGccattgcgcg | |
| anti-anti-terminator | -14.5 | 83635 | 83686 | gttcgctttaTCGGTCTACTACTACTAAACGCATCTTCTTTGCGCGGTAGACGAGTGAGCGgcatccagca |
| Notes: "The provided "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appear as the reverse complement of the sequence delimited by LeftPos-RigtPos" |
| REGULATION, RNA cis-regulatory element: | |
| Rfam type: | Cis-reg; leader |
| Strand: | reverse |
| Description | Rfam score | Left Pos | Right Pos | Sequence (RNA-strand) | |
|---|---|---|---|---|---|
| Leucine operon leader | 145.2 | 83573 | 83722 | ucgcauucauCUGGAGCUGAUUUAAUGACUCACAUCGUUCGCUUUAUCGGUCUACUACUACUAAACGCAUCUUCUUUGCGCGGUAGACGAGUGAGCGGCAUCCAGCAUUAAGCCAGCACGCAGUCAAACAAAAAACCCGCGCCAUUGCGCGGGUUUUUUUaugcccgaag |
| Notes: "The provied "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appears as the reverse complement of the sequence delimited by LeftPos-RightPos" |
| Reference(s) |
|
|---|---|
[CV(RS-EPT-CBR)] cross validation(RS-EPT-CBR)