RegulonDB RegulonDB 10.8: Operon Form
   

glyA operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: glyA
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: glyA
Synonym(s): OP00267
Gene(s): glyA   Genome Browser M3D Gene expression COLOMBOS
Note(s): Lorenz et al. in 1996 showed that MetR at low concentrations represses the glyA gene, when it binds on the binding site located at -142 Lorenz E,1996, and simultaneously activates the transcription of the hmp gene Membrillo-Hernandez J,1998. In addition, MetR functions as an activator when it binds as a dimer of dimers to binding sites located at -121 and -143 relative to the transcription start site of glyA.
Based on gene expression analysis, it was shown that CsgD could positively regulate glyA expression Herrington MB,2013
Reference(s): [1] Lorenz E., et al., 1995
[2] Steiert JG., et al., 1992
Promoter
Name: glyAp
+1: 2685574
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 67
Sequence: cgtttgcgtaaattcctttgtcaagacctgttatcgcacaatgattcggttatactgttcGccgttgtccaacaggaccgc
                            -35                    -10      +1                   
Evidence: [CV(RPF)]
[CV(RPF/TIM)]
[CV(TIM)]
[HIPP]
[RPF]
[TIM]
Reference(s): [3] Plamann MD., et al., 1983
Terminator(s)
Type: rho-independent
Sequence: taacgtagaaAGGCTTCCCGAAGGAAGCCttgatgatca
Reference(s): [4] Plamann MD., et al., 1983
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote MetR-L-homocysteine activator glyAp 2685688 2685702 -121.0 catcacaaacCTGAAAAGAAATCCGtttccggttg nd [APIORCISFBSCS], [BPP], [CV(GEA)], [CV(GEA)], [CV(GEA/SM)], [CV(SM)], [GEA], [SM] [1], [5], [6], [7]
remote MetR-L-homocysteine activator glyAp 2685710 2685724 -143.0 atgtgatgaaGTGAAAAATTTGCATcacaaacctg nd [APIORCISFBSCS], [BPP], [CV(GEA)], [CV(GEA)], [CV(GEA/SM)], [CV(SM)], [GEA], [IMP], [SM] [1], [5], [6], [7], [8]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote MetR-L-homocysteine repressor glyAp 2685710 2685724 -143.0 atgtgatgaaGTGAAAAATTTGCATcacaaacctg nd [APIORCISFBSCS], [BPP], [CV(GEA)], [CV(GEA)], [CV(GEA/SM)], [CV(SM)], [GEA], [IMP], [SM] [1], [5], [6], [7], [8]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal PurR-hypoxanthine repressor glyAp 2685626 2685641 -59.5 cgtagcctgaAGGTAATCGTTTGCGTaaattccttt nd [AIBSCS], [APIORCISFBSCS], [BPP], [CV(GEA)], [CV(GEA)], [CV(GEA/SM)], [CV(SM)], [GEA], [SM] [1], [2], [5], [9]




Reference(s)    

 [1] Lorenz E., Stauffer GV., 1995, Characterization of the MetR binding sites for the glyA gene of Escherichia coli., J Bacteriol 177(14):4113-20

 [2] Steiert JG., Kubu C., Stauffer GV., 1992, The PurR binding site in the glyA promoter region of Escherichia coli., FEMS Microbiol Lett 78(2-3):299-304

 [3] Plamann MD., Stauffer GV., 1983, Characterization of the Escherichia coli gene for serine hydroxymethyltransferase., Gene 22(1):9-18

 [4] Plamann MD., Stauffer LT., Urbanowski ML., Stauffer GV., 1983, Complete nucleotide sequence of the E. coli glyA gene., Nucleic Acids Res 11(7):2065-75

 [5] Lorenz E., Stauffer GV., 1996, RNA polymerase, PurR and MetR interactions at the glyA promoter of Escherichia coli., Microbiology 142 ( Pt 7):1819-24

 [6] Lorenz E., Stauffer GV., 1996, MetR-mediated repression of the glyA gene in Escherichia coli., FEMS Microbiol Lett 144(2-3):229-33

 [7] Lorenz E., Stauffer GV., 1996, Cooperative MetR binding in the Escherichia coli glyA control region., FEMS Microbiol Lett 137(2-3):147-52

 [8] Membrillo-Hernandez J., Coopamah MD., Channa A., Hughes MN., Poole RK., 1998, A novel mechanism for upregulation of the Escherichia coli K-12 hmp (flavohaemoglobin) gene by the 'NO releaser', S-nitrosoglutathione: nitrosation of homocysteine and modulation of MetR binding to the glyA-hmp intergenic region., Mol Microbiol 29(4):1101-12

 [9] Cho BK., Federowicz SA., Embree M., Park YS., Kim D., Palsson BO., 2011, The PurR regulon in Escherichia coli K-12 MG1655., Nucleic Acids Res 39(15):6456-64


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