RegulonDB RegulonDB 10.8: Operon Form
   

nadB operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: nadB
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: nadB
Synonym(s): OP00272
Gene(s): nadB   Genome Browser M3D Gene expression COLOMBOS
Reference(s): [1] Otsuka J., et al., 1996
Promoter
Name: nadBp
+1: 2710357
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 63
Sequence: tttttctcgccgccctgcgtcagcgtgtttagcaactgtaacaaatattaaaatagcaggTgtttatccgcacaacatgat
                            -35                  -10        +1                   
Evidence: [AIPP]
Reference(s): [2] Huerta AM., et al., 2003
TF binding sites (TFBSs)
[NadR,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote NadR repressor nadBp 2710396 2710413 48.5 gaccaaaccaTGTTTAGTAAATTAAACAaagaaaatga nd [AIBSCS], [BPP], [CV(GEA)], [CV(GEA)], [GEA], [IMP] [1], [3], [4], [5]


Evidence    

 [AIPP] Automated inference of promoter position

 [AIBSCS] Automated inference based on similarity to consensus sequences

 [BPP] Binding of purified proteins

 [CV(GEA)] cross validation(GEA)

 [GEA] Gene expression analysis

 [IMP] Inferred from mutant phenotype


Reference(s)    

 [1] Otsuka J., Watanabe H., Mori KT., 1996, Evolution of transcriptional regulation system through promiscuous coupling of regulatory proteins with operons; suggestion from protein sequence similarities in Escherichia coli., J Theor Biol 178(2):183-204

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [3] Gerasimova AV., Gelfand MS., 2005, Evolution of the NadR regulon in Enterobacteriaceae., J Bioinform Comput Biol 3(4):1007-19

 [4] Raffaelli N., Lorenzi T., Mariani PL., Emanuelli M., Amici A., Ruggieri S., Magni G., 1999, The Escherichia coli NadR regulator is endowed with nicotinamide mononucleotide adenylyltransferase activity., J Bacteriol 181(17):5509-11

 [5] Tritz GJ., Chandler JL., 1973, Recognition of a gene involved in the regulation of nicotinamide adenine dinucleotide biosynthesis., J Bacteriol 114(1):128-36

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