RegulonDB RegulonDB 10.8: Operon Form
   

codBA operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: codBA
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: codBA
Synonym(s): OP00277
Gene(s): codB, codA   Genome Browser M3D Gene expression COLOMBOS
Note(s): The expression of the codBA operon, whose genes overlap, is induced under nitrogen-limited growth conditions and repressed by purines or pyrimidines. This induction and repression are mediated by NAC and PurR proteins, respectively Muse WB,2003. Danielsen S,1992.
Evidence: [ITC] Inferred through co-regulation
[PM] Polar mutation
Reference(s): [1] Danielsen S., et al., 1992
Promoter
Name: codBp
+1: 354884
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 38
Sequence: aaaaatatatttccccacgaaaacgattgctttttatcttcagatgaatagaatgcggcgGattttttgggtttcaaacag
                           -35                   -10        +1                   
Note(s): There are two codBA transcriptional start positions, G7 and A8, relative to the -10 box. A8 always leads to reiterative transcription and the synthesis of transcripts with sequence AUUUUn, which are not extended downstream to include the codBA structural gene sequences. The G7 does not engage in reiterative transcription, which permits them to be extended downstream to generate productive codBA mRNA Qi F,1995.
Evidence: [HIPP]
[TIM]
Reference(s): [2] Qi F., et al., 1995
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal Nac1 activator codBp 354819 354833 -58.0 gcactcattcATATAAAAAATATATttccccacga nd [AIBSCS], [BPP], [CV(GEA)], [CV(GEA)], [CV(GEA/SM)], [CV(SM)], [GEA], [SM] [3], [4]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal PurR-hypoxanthine1 repressor codBp 354840 354855 -36.5 atatttccccACGAAAACGATTGCTTtttatcttca nd [AIBSCS], [APIORCISFBSCS], [CV(GEA)], [GEA] [1], [5], [6]
Note(s): 1In a footprinting analysis the Nac protein of Klebsiella pneumoniae was used.1This PurR-binding site overlaps the -35 box of the codBp ptomoter Danielsen S,1992.1In a footprinting analysis the Nac protein of Klebsiella pneumoniae was used.
2This PurR-binding site overlaps the -35 box of the codBp ptomoter Danielsen S,1992.





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