RegulonDB RegulonDB 10.6.3: Operon Form
   

glpTQ operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: glpTQ
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: glpTQ
Synonym(s): OP00304
Gene(s): glpQ, glpT   Genome Browser M3D Gene expression COLOMBOS
Note(s): GlpR bound to this region represses the glpTQ operon, and this repression depends on the GlpR-binding sites which are close to the glpABC operon, a divergent operon of glpTQ. All these GlpR sites possibly form a looped nucleoprotein complex |CITS:[9179845]|.
|CITS:[ 1556120]| identified five GlpR-binding sites upstream of the glpTQ operon. However , |CITS: [9179845]|, using footprinting analysis, concluded that one of them (at -41.5) is not an operator.
It was suggested that IHF represses the glpTQ operon by facilitating formation of a repression loop, in which GlpR is also a member of the complex.
Reference(s): [1] Larson TJ., et al., 1992
Promoter
Name: glpTQp
+1: 2352451
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 77
Sequence: atttaataatgtgtgcggcaattcacatttaatttatgaatgttttcttaacatcgcggcAactcaagaaacggcaggttc
                      -10                        -35        +1                   
Evidence: [HIPP]
Reference(s): [1] Larson TJ., et al., 1992
[2] Yang B., et al., 1997
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal CRP-cAMP activator glpTQp 2352482 2352503 -41.5 ttatttaataATGTGTGCGGCAATTCACATTTaatttatgaa nd [AIBSCS], [BPP], [GEA], [HIBSCS] [1], [2], [5], [8]
proximal CRP-cAMP activator glpTQp 2352532 2352553 -91.5 ttcgaaagtgAAACGTGATTTCATGCGTCATTttgaacattt nd [BPP], [GEA], [HIBSCS] [1], [2], [5]
remote CRP-cAMP activator glpTQp 2352572 2352593 -131.5 aaaacatgaaTTGTTTGATTTCGCGCATATTCgctcataatt nd [BPP], [GEA], [HIBSCS] [1], [2], [5]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal FNR activator glpTQp 2352536 2352549 -91.5 aaagtgaaacGTGATTTCATGCGTcattttgaac nd [AIBSCS], [GEA] [2], [6], [7]
remote FNR activator glpTQp 2352576 2352589 -131.5 catgaattgtTTGATTTCGCGCATattcgctcat nd [GEA], [HIBSCS] [2], [6], [7]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote Fis activator glpTQp 2352396 2352410 49.0 tcaggctgttAATCATAAATAAGACcacgggccac nd [AIBSCS], [GEA] [4]
proximal Fis activator glpTQp 2352460 2352474 -16.0 tttaatttatGAATGTTTTCTTAACatcgcggcaa nd [AIBSCS], [GEA] [4]
proximal Fis activator glpTQp 2352508 2352522 -64.0 tttgaacattTTGTAAATCTTATTTaataatgtgt nd [AIBSCS], [GEA] [4]
proximal Fis activator glpTQp 2352515 2352529 -71.0 gcgtcattttGAACATTTTGTAAATcttatttaat nd [AIBSCS], [GEA] [4]
remote Fis activator glpTQp 2352557 2352571 -113.0 gcgcatattcGCTCATAATTCGAAAgtgaaacgtg nd [AIBSCS], [GEA] [4]
remote Fis activator glpTQp 2352610 2352624 -166.0 gaagttcgttTTTTACCATTTAGCCatagtaaaaa nd [AIBSCS], [GEA] [4]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote GlpR repressor glpTQp 2352094 2352113 348.5 ttcggtatcgGATCGCTCGAATCCGCGCGTtttcctgccc nd [BPP], [GEA], [SM] [2]
remote GlpR repressor glpTQp 2352109 2352128 333.5 gaaattcatcATGGGTTCGGTATCGGATCGctcgaatccg nd [BPP], [GEA], [SM] [2]
remote GlpR repressor glpTQp 2352127 2352146 315.5 gattgcttatGGATTTTCGAAATTCATCATgggttcggta nd [BPP], [GEA], [SM] [2]
proximal GlpR repressor glpTQp 2352483 2352502 -41.5 tatttaataaTGTGTGCGGCAATTCACATTtaatttatga nd [BPP], [HIBSCS], [SM] [1], [3]
proximal GlpR repressor glpTQp 2352523 2352542 -81.5 aacgtgatttCATGCGTCATTTTGAACATTttgtaaatct nd [BPP], [HIBSCS], [SM] [1], [2], [3]
remote GlpR repressor glpTQp 2352555 2352574 -113.5 ttcgcgcataTTCGCTCATAATTCGAAAGTgaaacgtgat nd [BPP], [HIBSCS], [SM] [1], [2], [3]
remote GlpR repressor glpTQp 2352574 2352593 -132.5 aaaacatgaaTTGTTTGATTTCGCGCATATtcgctcataa nd [BPP], [HIBSCS], [SM] [1], [2], [3]
remote GlpR repressor glpTQp 2352614 2352633 -172.5 tatccctctgAAGTTCGTTTTTTACCATTTagccatagta nd [BPP], [HIBSCS], [SM] [1], [2], [3]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote IHF repressor glpTQp 2352244 2352256 202.0 ggctatgcggCTTACTATTTGGTtcgtaagaac nd [BPP], [GEA], [HIBSCS] [2]
remote IHF repressor glpTQp 2352411 2352423 35.0 ttctctcactGAATCAGGCTGTTaatcataaat nd [BPP], [GEA], [HIBSCS] [2]




Reference(s)    

 [1] Larson TJ., Cantwell JS., van Loo-Bhattacharya AT., 1992, Interaction at a distance between multiple operators controls the adjacent, divergently transcribed glpTQ-glpACB operons of Escherichia coli K-12., J Biol Chem 267(9):6114-21

 [2] Yang B., Gerhardt SG., Larson TJ., 1997, Action at a distance for glp repressor control of glpTQ transcription in Escherichia coli K-12., Mol Microbiol 24(3):511-21

 [3] Zhao N., Oh W., Trybul D., Thrasher KS., Kingsbury TJ., Larson TJ., 1994, Characterization of the interaction of the glp repressor of Escherichia coli K-12 with single and tandem glp operator variants., J Bacteriol 176(8):2393-7

 [4] Bradley MD., Beach MB., de Koning AP., Pratt TS., Osuna R., 2007, Effects of Fis on Escherichia coli gene expression during different growth stages., Microbiology 153(Pt 9):2922-40

 [5] Weissenborn DL., Wittekindt N., Larson TJ., 1992, Structure and regulation of the glpFK operon encoding glycerol diffusion facilitator and glycerol kinase of Escherichia coli K-12., J Biol Chem 267(9):6122-31

 [6] Kuritzkes DR., Zhang XY., Lin EC., 1984, Use of phi(glp-lac) in studies of respiratory regulation of the Escherichia coli anaerobic sn-glycerol-3-phosphate dehydrogenase genes (glpAB)., J Bacteriol 157(2):591-8

 [7] Wong KK., Kwan HS., 1992, Transcription of glpT of Escherichia coli K12 is regulated by anaerobiosis and fnr., FEMS Microbiol Lett 73(1-2):15-8

 [8] Zheng D., Constantinidou C., Hobman JL., Minchin SD., 2004, Identification of the CRP regulon using in vitro and in vivo transcriptional profiling., Nucleic Acids Res 32(19):5874-93


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