RegulonDB RegulonDB 10.9: Operon Form

metF operon and associated TUs in Escherichia coli K-12 genome

Name: metF
This page displays every known transcription unit of this operon and their known regulation.

Transcription unit          
Name: metF
Synonym(s): OP00308
Gene(s): metF   Genome Browser M3D Gene expression COLOMBOS
Note(s): The regulatory effect of MetJ on the promoter metFp is as yet not known. However, we assigned a negative effect to this regulatory interaction based on two facts: the protein's binding site is very close to the promoter, and MetJ is known to act, so far without exceptions, as a negative regulator.
Reference(s): [1] Greene RC., et al., 1984
Name: metFp
+1: 4132546
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 70
Sequence: tttcctcaccatcattggtcatttttcggttgacgcccttcggcttttccttcatctttaCatctggacgtctaaacggat
                              -35                    -10    +1                   
Evidence: [ICWHO]
Reference(s): [2] Huerta AM., et al., 2003
[3] Saint-Girons I., et al., 1983
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal MetJ-S-adenosyl-L-methionine repressor metFp 4132530 4132545 -8.5 gcccttcggcTTTTCCTTCATCTTTACatctggacgt nd [BCE], [SM] nd
proximal MetJ-S-adenosyl-L-methionine repressor metFp 4132539 4132554 2.0 cttttccttcATCTTTACATCTGGACGtctaaacgga nd [AIBSCS] [4]
proximal MetJ-S-adenosyl-L-methionine repressor metFp 4132542 4132557 4.5 ttccttcatcTTTACATCTGGACGTCTaaacggatag nd [BCE], [SM] nd
proximal MetJ-S-adenosyl-L-methionine repressor metFp 4132545 4132560 7.5 cttcatctttACATCTGGACGTCTAAAcggatagatg nd [AIBSCS] [4]
proximal MetJ-S-adenosyl-L-methionine repressor metFp 4132547 4132562 9.5 tcatctttacATCTGGACGTCTAAACGgatagatgtg nd [AIBSCS] [4]
proximal MetJ-S-adenosyl-L-methionine repressor metFp 4132555 4132570 17.5 acatctggacGTCTAAACGGATAGATGtgcacaacac nd [AIBSCS] [4]
proximal MetJ-S-adenosyl-L-methionine repressor metFp 4132558 4132573 20.5 tctggacgtcTAAACGGATAGATGTGCacaacacaac nd [BCE], [SM] nd

RNA cis-regulatory element    
Regulation, transcriptional elongation  
Attenuator type: Transcriptional
Strand: forward
  Structure type Energy LeftPos RightPos Sequence (RNA-strand)
  terminator -13.4 4132416 4132453 tatatttgcaGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTaccatcctga
  anti-terminator -6.8 4132383 4132430 atccggcaagAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTgcataccgga
Notes: "The provided "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appear as the reverse complement of the sequence delimited by LeftPos-RigtPos"