RegulonDB RegulonDB 10.8: Operon Form
   

argE operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: argE
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: argE
Synonym(s): OP00321
Gene(s): argE   Genome Browser M3D Gene expression COLOMBOS
Reference(s): [1] Natter W., et al., 1977
[2] Sens D., et al., 1977
Promoter
Name: argEp1
+1: 4154897
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 50
Sequence: aaaacattcaccttacggctggtgggttttattacgctcaacgttagtgtatttttattcAtaaatactgcatgaatattg
                            -35                   -10       +1                   
Evidence: [CV(RS-EPT-CBR)]
[CV(TIM)]
[HIPP]
[RS-EPT-CBR]
[TIM]
Reference(s): [3] Cunin R., et al., 1983
[4] Piette J., et al., 1982
[5] Salgado H, et al., 2012
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal ArgR-L-arginine repressor argEp1 4154873 4154890 16.5 ttcataaataCTGCATGAATATTGATACtatcatgacc nd [AIBSCS], [BCE], [BPP], [CV(GEA)], [CV(GEA)], [CV(GEA/SM)], [CV(SM)], [GEA], [SM] [3], [6], [7], [8]
proximal ArgR-L-arginine repressor argEp1 4154895 4154912 -6.5 gctcaacgttAGTGTATTTTTATTCATAaatactgcat nd [AIBSCS], [BCE], [BPP], [CV(GEA)], [CV(GEA)], [CV(GEA/SM)], [CV(SM)], [GEA], [SM] [3], [6], [7], [8]


Transcription unit       
Name: argE
Gene(s): argE   Genome Browser M3D Gene expression COLOMBOS
Evidence: [ICWHO] Inferred computationally without human oversight
Promoter
Name: argEp2
+1: 4155078
Distance from start of the gene: 231
Sequence: ggagattaactttcccgcatcattgctttgcgctgaaacagtcaaagcggttatgttcatAtgcggatggcgatttacata
Evidence: [TIM]
Reference(s): [3] Cunin R., et al., 1983
[4] Piette J., et al., 1982




Reference(s)    

 [1] Natter W., Sens D., James E., 1977, Metabolism of arginine-specific messenger ribonucleic acid in Escherichia coli K-12., J Bacteriol 131(1):214-23

 [2] Sens D., Natter W., James E., 1977, In vitro transcription of the Escherichia coli K-12 argA, argE, and argCBH operons., J Bacteriol 130(2):642-55

 [3] Cunin R., Eckhardt T., Piette J., Boyen A., Pierard A., Glansdorff N., 1983, Molecular basis for modulated regulation of gene expression in the arginine regulon of Escherichia coli K-12., Nucleic Acids Res 11(15):5007-19

 [4] Piette J., Cunin R., Boyen A., Charlier D., Crabeel M., Van Vliet F., Glansdorff N., Squires C., Squires CL., 1982, The regulatory region of the divergent argECBH operon in Escherichia coli K-12., Nucleic Acids Res 10(24):8031-48

 [5] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [6] Caldara M., Charlier D., Cunin R., 2006, The arginine regulon of Escherichia coli: whole-system transcriptome analysis discovers new genes and provides an integrated view of arginine regulation., Microbiology 152(Pt 11):3343-54

 [7] Charlier D., Roovers M., Van Vliet F., Boyen A., Cunin R., Nakamura Y., Glansdorff N., Pierard A., 1992, Arginine regulon of Escherichia coli K-12. A study of repressor-operator interactions and of in vitro binding affinities versus in vivo repression., J Mol Biol 226(2):367-86

 [8] Makarova KS., Mironov AA., Gelfand MS., 2001, Conservation of the binding site for the arginine repressor in all bacterial lineages., Genome Biol 2(4):RESEARCH0013


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