RegulonDB RegulonDB 10.7: Operon Form
   

gntT operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: gntT
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: gntT
Synonym(s): OP00324
Gene(s): gntT   Genome Browser M3D Gene expression COLOMBOS
Evidence: [LTED] Length of transcript experimentally determined
[LTED] Length of transcript experimentally determined
Reference(s): [1] Porco A., et al., 1997
Promoter
Name: gntTp1
+1: 3546405
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 154
Sequence: tttaaatttaccccgctctggtgattctcaaacgccagatgttacccgtatcattcacatGggtaccaaacatactcctga
                         -35                     -10        +1                   
Evidence: [TIM] ; Method: Primer Extension
[TIM]
Reference(s): [2] Izu H., et al., 1997
[1] Porco A., et al., 1997
TF binding sites (TFBSs)
[CRP,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal CRP-cAMP activator gntTp1 3546323 3546344 -71.5 ttccccgcgaTAATATGACCAACCTCTCATAAtttaaattta nd [APIORCISFBSCS], [BPP], [GEA], [SM], [TASES] [2], [3], [4], [5]
[GntR,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal GntR repressor gntTp1 3546381 3546400 -14.5 ctcaaacgccAGATGTTACCCGTATCATTCacatgggtac nd [AIBSCS], [APIORCISFBSCS], [BPP], [GEA], [TASES] [2], [3], [4], [5], [6]
remote GntR repressor gntTp1 3546522 3546541 127.5 gtcagaaaatTGACGTTACCCATAACAAATgaaaggccag nd [AIBSCS], [APIORCISFBSCS], [BPP], [GEA], [TASES] [3], [4], [5], [6]


Transcription unit          
Name: gntT
Gene(s): gntT   Genome Browser M3D Gene expression COLOMBOS
Evidence: [LTED] Length of transcript experimentally determined
[LTED] Length of transcript experimentally determined
Reference(s): [1] Porco A., et al., 1997
Promoter
Name: gntTp2
+1: 3546446
Distance from start of the gene: 113
Sequence: ttacccgtatcattcacatgggtaccaaacatactcctgacatctgactacaataattagTtttagtgggtatcagtcgtg
Evidence: [TIM]
Reference(s): [2] Izu H., et al., 1997
TF binding sites (TFBSs)
[CRP,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote CRP-cAMP activator gntTp2 3546323 3546344 -112.5 ttccccgcgaTAATATGACCAACCTCTCATAAtttaaattta nd [APIORCISFBSCS], [BPP], [GEA], [SM], [TASES] [2], [3], [4], [5]
[GntR,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal GntR repressor gntTp2 3546381 3546400 -55.5 ctcaaacgccAGATGTTACCCGTATCATTCacatgggtac nd [AIBSCS], [APIORCISFBSCS], [BPP], [GEA], [TASES] [2], [3], [4], [5], [6]
remote GntR repressor gntTp2 3546522 3546541 86.5 gtcagaaaatTGACGTTACCCATAACAAATgaaaggccag nd [AIBSCS], [APIORCISFBSCS], [BPP], [GEA], [TASES] [3], [4], [5], [6]


Transcription unit          
Name: gntT
Gene(s): gntT   Genome Browser M3D Gene expression COLOMBOS
Evidence: [LTED] Length of transcript experimentally determined
[LTED] Length of transcript experimentally determined
Reference(s): [1] Porco A., et al., 1997
Promoter
Name: gntTp3
+1: 3546449
Distance from start of the gene: 110
Sequence: cccgtatcattcacatgggtaccaaacatactcctgacatctgactacaataattagtttTagtgggtatcagtcgtggtg
Evidence: [TIM]
Reference(s): [2] Izu H., et al., 1997
TF binding sites (TFBSs)
[CRP,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote CRP-cAMP activator gntTp3 3546323 3546344 -115.5 ttccccgcgaTAATATGACCAACCTCTCATAAtttaaattta nd [APIORCISFBSCS], [BPP], [GEA], [SM], [TASES] [2], [3], [4], [5]
[GntR,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal GntR repressor gntTp3 3546381 3546400 -58.5 ctcaaacgccAGATGTTACCCGTATCATTCacatgggtac nd [AIBSCS], [APIORCISFBSCS], [BPP], [GEA], [TASES] [2], [3], [4], [5], [6]
remote GntR repressor gntTp3 3546522 3546541 83.5 gtcagaaaatTGACGTTACCCATAACAAATgaaaggccag nd [AIBSCS], [APIORCISFBSCS], [BPP], [GEA], [TASES] [3], [4], [5], [6]


Evidence    

 [TIM] Transcription initiation mapping

 [APIORCISFBSCS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.

 [BPP] Binding of purified proteins

 [GEA] Gene expression analysis

 [SM] Site mutation

 [TASES] Traceable author statement to experimental support

 [AIBSCS] Automated inference based on similarity to consensus sequences


Reference(s)    

 [1] Porco A., Peekhaus N., Bausch C., Tong S., Isturiz T., Conway T., 1997, Molecular genetic characterization of the Escherichia coli gntT gene of GntI, the main system for gluconate metabolism., J Bacteriol 179(5):1584-90

 [2] Izu H., Kawai T., Yamada Y., Aoshima H., Adachi O., Yamada M., 1997, Characterization of the gntT gene encoding a high-affinity gluconate permease in Escherichia coli., Gene 199(1-2):203-10

 [3] Peekhaus N., Conway T., 1998, Positive and negative transcriptional regulation of the Escherichia coli gluconate regulon gene gntT by GntR and the cyclic AMP (cAMP)-cAMP receptor protein complex., J Bacteriol 180(7):1777-85

 [4] Peekhaus N., Conway T., 1998, What's for dinner?: Entner-Doudoroff metabolism in Escherichia coli., J Bacteriol 180(14):3495-502

 [5] Rodionov DA., Mironov AA., Rakhmaninova AB., Gelfand MS., 2000, Transcriptional regulation of transport and utilization systems for hexuronides, hexuronates and hexonates in gamma purple bacteria., Mol Microbiol 38(4):673-83

 [6] Suvorova IA., Korostelev YD., Gelfand MS., 2015, GntR Family of Bacterial Transcription Factors and Their DNA Binding Motifs: Structure, Positioning and Co-Evolution., PLoS One 10(7):e0132618

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