RegulonDB RegulonDB 10.10: Operon Form
   

accA operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: accA
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: accA
Gene(s): accA   Genome Browser M3D Gene expression COLOMBOS
Evidence: [LTED] Length of transcript experimentally determined
Reference(s): [1] Li SJ., et al., 1993
Promoter
Name: accAp2
+1: 208326
Distance from start of the gene: 295
Sequence: acttatcgtcagcggacaggtcagctttgatgacttcagcggtgggcttaaaatgaccgcTcgcgaagtgatggatattga
Evidence: [RS-EPT-CBR]
[TIM]
Reference(s): [2] My L., et al., 2015
[3] Salgado H, et al., 2012
TF binding sites (TFBSs)
[FadR,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal FadR activator accAp2 208280 208296 -38.0 atcgtcagcgGACAGGTCAGCTTTGATgacttcagcg nd [GEA], [AIBSCS], [BPP] [2]


Transcription unit       
Name: accA
Gene(s): accA   Genome Browser M3D Gene expression COLOMBOS
Note(s): The accA gene is located at min 4.3 in a complex gene cluster. The messages observed may not be primary transcription products (the presence of 5'-triphosphates was not demonstrated); however, each of the 5' ends lies downstream of a reasonable -10 promoter element, strongly suggesting that nucleotide processing of the 5' ends occurrs.
The accAp promoter identified by 5'-RACE by My et al. My L,2015is 1 bp upstream from a promoter identified previously Li SJ,1993
Evidence: [LTED] Length of transcript experimentally determined
Reference(s): [1] Li SJ., et al., 1993
Promoter
Name: accAp1
+1: 208411
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 210
Sequence: gcccgggaaaaatatgctcgcgggcttgctatctcgctgacggacaggcaaattgatgacCagcttttaaaccgactccgt
                          -35                    -10        +1                   
Evidence: [ICWHO]
[TIM]
Reference(s): [4] Huerta AM., et al., 2003
[1] Li SJ., et al., 1993
[2] My L., et al., 2015


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [TIM] Transcription initiation mapping

 [GEA] Gene expression analysis

 [AIBSCS] Automated inference based on similarity to consensus sequences

 [BPP] Binding of purified proteins

 [ICWHO] Inferred computationally without human oversight


Reference(s)    

 [1] Li SJ., Cronan JE., 1993, Growth rate regulation of Escherichia coli acetyl coenzyme A carboxylase, which catalyzes the first committed step of lipid biosynthesis., J Bacteriol 175(2):332-40

 [2] My L., Ghandour Achkar N., Viala JP., Bouveret E., 2015, Reassessment of the Genetic Regulation of Fatty Acid Synthesis in Escherichia coli: Global Positive Control by the Dual Functional Regulator FadR., J Bacteriol 197(11):1862-72

 [3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [4] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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