RegulonDB RegulonDB 10.8: Operon Form
   

btuCED operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: btuCED
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit       
Name: btuD
Gene(s): btuD   Genome Browser M3D Gene expression COLOMBOS
Evidence: [IEP] Inferred from expression pattern
Reference(s): [1] Li Z., et al., 2019
Promoter
Name: btuDp
+1: 1793583
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 25
Sequence: gaaaagtcatccagcgtttttccccggatatgacgccggaagatcccattgtgatggaaaGcattaaactggcgttggcaa
                         -35                      -10       +1                   
Evidence: [RE]
[TIM]
Reference(s): [1] Li Z., et al., 2019


Transcription unit       
Name: btuE
Gene(s): btuE   Genome Browser M3D Gene expression COLOMBOS
Evidence: [IEP] Inferred from expression pattern
Reference(s): [1] Li Z., et al., 2019
Promoter
Name: btuEp
+1: 1794124
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 15
Sequence: agcaggacgttagccgcaaaaagacggtctatgattaaaagctaagatttttactgaccaCacccaggagaaacgatgcaa
                          -35                     -10       +1                   
Evidence: [RE]
[TIM]
Reference(s): [1] Li Z., et al., 2019


Transcription unit       
Name: btuE
Gene(s): btuE   Genome Browser M3D Gene expression COLOMBOS
Evidence: [IEP] Inferred from expression pattern
Reference(s): [1] Li Z., et al., 2019
Promoter
Name: btuEp2
+1: 1794129
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 20
Sequence: ttaaaagcaggacgttagccgcaaaaagacggtctatgattaaaagctaagatttttactGaccacacccaggagaaacga
                          -35                      -10      +1                   
Evidence: [RE]
[TIM]
Reference(s): [1] Li Z., et al., 2019


Transcription unit       
Name: btuCED
Gene(s): btuD, btuE, btuC   Genome Browser M3D Gene expression COLOMBOS
Reference(s): [2] Rioux CR., et al., 1989


RNA cis-regulatory element    
Regulation, transcriptional elongation  
Attenuator type: Transcriptional
Strand: reverse
  Structure type Energy LeftPos RightPos Sequence (RNA-strand)
  terminator -15.3 1795210 1795240 tgatctaactAAAAAGGCCGCTCTGCGGCCTTTTTTCTTTtcactgtcga
Notes: "The provided "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appear as the reverse complement of the sequence delimited by LeftPos-RigtPos"


Evidence    

 [RE] RNA-seq evidence

 [TIM] Transcription initiation mapping


Reference(s)    

 [1] Li Z., Pan Q., Xiao Y., Fang X., Shi R., Fu C., Danchin A., You C., 2019, Deciphering global gene expression and regulation strategy in Escherichia coli during carbon limitation., Microb Biotechnol 12(2):360-376

 [2] Rioux CR., Kadner RJ., 1989, Vitamin B12 transport in Escherichia coli K12 does not require the btuE gene of the btuCED operon., Mol Gen Genet 217(2-3):301-8

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