RegulonDB RegulonDB 10.10: Operon Form
   

chbBCARFG operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: chbBCARFG
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: chbBCARFG
Gene(s): chbG, chbF, chbR, chbA, chbC, chbB   Genome Browser M3D Gene expression COLOMBOS
Note(s): chbBCARFG is a cryptic operon Parker LL,1990involved in the catabolism of N,N'-diacetylchitobiose Keyhani NO,1997 A Cel+ phenotype was conferred by mutations which acted concertedly to alter the regulation of this operon Kachroo AH,2007
Plumbridge and Pelligrini (2004) proposed a model for chbBCARFG expression in which they mentioned that when the operon is repressed, NagC and ChbR sites are occupied, probably forming a complex structure involving an interaction between the two NagC proteins and formation of a DNA loop. It could be that cAMP/CRP is present in the complex. When the cell is grown on chitobiose, it generates the inducing signals for both ChbR and NagC. Transport of chitobiose produces chitobiose-6P. Its subsequent metabolism generates GlcNAc-6P, the inducer for NagC, which displaces NagC from its sites. The unidentified inducer for ChbR converts ChbR into an activator in the presence of cAMP/CAP and allows RNA polymerase to bind and transcribe the chb operon Plumbridge J,2004
Based on cells with plasmids and three inducible promoters of genes (matA, safA, and chbB), three novel fluorescent biosensors sensitive to terahertz (THz) radiation were developed, and they controlled the expression of a fluorescent protein Serdyukov DS, Goryachkovskaya TN, Mescheryakova IA, Bannikova SV, Kuznetsov SA, Cherkasova OP, Popik VM, Peltek SE,2020 The induction of these biosensors was manifested as fluorescent luminosity and reflected the activity of transcription units matA, safA-ydeO, and chbBCARFG, which contain the transcription factors MatA, YdeO, and ChbR, respectively Serdyukov DS, Goryachkovskaya TN, Mescheryakova IA, Bannikova SV, Kuznetsov SA, Cherkasova OP, Popik VM, Peltek SE,2020
Reference(s): [1] Keyhani NO., et al., 1997
[2] Reizer J., et al., 1990
Promoter
Name: chbBp
+1: 1821726
Distance from start of the gene: 107
Sequence: gaaacaatttataaggtcaaagaaatactattgctcaggtctataccgtatactcctttcAgccacaaaaaaagtcatgtt
Evidence: [TIM]
Reference(s): [3] Plumbridge J., et al., 2004
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal CRP-cyclic-AMP activator chbBp 1821799 1821820 -83.5 tatttgcccgAAATGTGAAGAGGGTCATAACCacaggtcaag nd [GEA], [AIBSCS], [APIORCISFBSCS], [BPP] [3], [4]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal ChbR-6-phospho-β-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine activator chbBp 1821763 1821764 -38.0 aacaatttatAAGGTCAAAGAAATACTATTgctcaggtct nd [GEA], [BPP] [3]
proximal ChbR-6-phospho-β-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine activator chbBp 1821786 1821787 -61.0 gtcataaccaCAGGTCAAGGAGAAACAATTtataaggtca nd [GEA], [BPP] [3]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal ChbR repressor chbBp 1821763 1821764 -38.0 nd nd [GEA], [BPP] [3]
proximal ChbR repressor chbBp 1821786 1821787 -61.0 nd nd [GEA], [BPP] [3]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal NagC repressor chbBp 1821715 1821737 1.0 tctataccgtATACTCCTTTCAGCCACAAAAAAagtcatgttg nd [GEA], [APIORCISFBSCS], [BPP] [3]
remote NagC repressor chbBp 1821827 1821849 -112.0 ggcctgagttCTTAATTATCTTCGCGAATTATTtgcccgaaat nd [GEA], [AIBSCS], [APIORCISFBSCS], [BPP], [SM] [3]


RNA cis-regulatory element    
Regulation, transcriptional elongation  
Attenuator type: Translational
Strand: reverse
Evidence: [ICA] Inferred by computational analysis
Reference(s): [7] Merino E, et al., 2005
  Structure type Energy LeftPos RightPos Sequence (RNA-strand)
  terminator -16.9 1819826 1819851 ttttaatcgaTGCCGGTACGGGTAATCGTGCCGGTaagaaaagag
  terminator -10.0 1821233 1821254 ataaatcaatACCGCAATATTTAAATTGCGGtttttaaggg
Notes: "The provided "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appear as the reverse complement of the sequence delimited by LeftPos-RigtPos"





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