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Name: | chbBCARFG |
Gene(s): | chbG, chbF, chbR, chbA, chbC, chbB Genome Browser M3D Gene expression COLOMBOS |
Note(s): | chbBCARFG is a cryptic operon Parker LL,1990involved in the catabolism of N,N'-diacetylchitobiose Keyhani NO,1997 A Cel+ phenotype was conferred by mutations which acted concertedly to alter the regulation of this operon Kachroo AH,2007 Plumbridge and Pelligrini (2004) proposed a model for chbBCARFG expression in which they mentioned that when the operon is repressed, NagC and ChbR sites are occupied, probably forming a complex structure involving an interaction between the two NagC proteins and formation of a DNA loop. It could be that cAMP/CRP is present in the complex. When the cell is grown on chitobiose, it generates the inducing signals for both ChbR and NagC. Transport of chitobiose produces chitobiose-6P. Its subsequent metabolism generates GlcNAc-6P, the inducer for NagC, which displaces NagC from its sites. The unidentified inducer for ChbR converts ChbR into an activator in the presence of cAMP/CAP and allows RNA polymerase to bind and transcribe the chb operon Plumbridge J,2004 |
Reference(s): |
[1] Keyhani NO., et al., 1997 [2] Reizer J., et al., 1990 |
Promoter | |
Name: | chbBp |
+1: | 1821726 |
Distance from start of the gene: | 107 |
Sequence: |
gaaacaatttataaggtcaaagaaatactattgctcaggtctataccgtatactcctttcAgccacaaaaaaagtcatgtt |
Evidence: | [TIM] |
Reference(s): | [3] Plumbridge J., et al., 2004 |
Type | Transcription factor | Function | Promoter | Binding Sites | Growth Conditions | Evidence (Confirmed, Strong, Weak) | Reference(s) | |||
---|---|---|---|---|---|---|---|---|---|---|
LeftPos | RightPos | Central Rel-Pos | Sequence | |||||||
proximal | CRP-cAMP | activator | chbBp | 1821799 | 1821820 | -83.5 | tatttgcccgAAATGTGAAGAGGGTCATAACCacaggtcaag | nd | [AIBSCS], [APIORCISFBSCS], [BPP], [CV(GEA)], [CV(GEA)], [GEA] | [3], [6] |
Type | Transcription factor | Function | Promoter | Binding Sites | Growth Conditions | Evidence (Confirmed, Strong, Weak) | Reference(s) | |||
---|---|---|---|---|---|---|---|---|---|---|
LeftPos | RightPos | Central Rel-Pos | Sequence | |||||||
proximal | ChbR-N-monoacetylchitobiose 6'-phosphate1 | activator | chbBp | 1821755 | 1821773 | -38.0 | acaatttataAGGTCAAAGAAATACTATTgctcaggtct | nd | [BPP], [GEA] | [3], [4] |
proximal | ChbR-N-monoacetylchitobiose 6'-phosphate2 | activator | chbBp | 1821778 | 1821796 | -61.0 | tcataaccacAGGTCAAGGAGAAACAATTtataaggtca | nd | [BPP], [GEA] | [3], [4] |
Type | Transcription factor | Function | Promoter | Binding Sites | Growth Conditions | Evidence (Confirmed, Strong, Weak) | Reference(s) | |||
---|---|---|---|---|---|---|---|---|---|---|
LeftPos | RightPos | Central Rel-Pos | Sequence | |||||||
proximal | ChbR1 | repressor | chbBp | 1821755 | 1821773 | -38.0 | acaatttataAGGTCAAAGAAATACTATTgctcaggtct | nd | [BPP], [GEA] | [3], [4] |
proximal | ChbR2 | repressor | chbBp | 1821778 | 1821796 | -61.0 | tcataaccacAGGTCAAGGAGAAACAATTtataaggtca | nd | [BPP], [GEA] | [3], [4] |
Type | Transcription factor | Function | Promoter | Binding Sites | Growth Conditions | Evidence (Confirmed, Strong, Weak) | Reference(s) | |||
---|---|---|---|---|---|---|---|---|---|---|
LeftPos | RightPos | Central Rel-Pos | Sequence | |||||||
proximal | NagC1 | repressor | chbBp | 1821715 | 1821737 | 1.0 | tctataccgtATACTCCTTTCAGCCACAAAAAAagtcatgttg | nd | [APIORCISFBSCS], [BPP], [CV(GEA)], [CV(GEA)], [GEA] | [3] |
remote | NagC2 | repressor | chbBp | 1821827 | 1821849 | -112.0 | ggcctgagttCTTAATTATCTTCGCGAATTATTtgcccgaaat | nd | [AIBSCS], [APIORCISFBSCS], [BPP], [CV(GEA)], [CV(GEA)], [CV(GEA/SM)], [CV(SM)], [GEA], [SM] | [3], [4], [5] |
Note(s): |
1ChbR and NagC appear to act as repressors for the chbB operon in the absence of the inducing sugar chitobiose Plumbridge J,2004 2ChbR and NagC appear to act as repressors for the chbB operon in the absence of the inducing sugar chitobiose Plumbridge J,20041ChbR and NagC appear to act as repressors for the chbB operon in the absence of the inducing sugar chitobiose Plumbridge J,2004 2ChbR and NagC appear to act as repressors for the chbB operon in the absence of the inducing sugar chitobiose Plumbridge J,20041NagC and ChbR appear to act as repressors for the chbB operon in the absence of the inducing sugar chitobiose Plumbridge J,2004. 2NagC and ChbR appear to act as repressors for the chbB operon in the absence of the inducing sugar chitobiose Plumbridge J,2004.1NagC and ChbR appear to act as repressors for the chbB operon in the absence of the inducing sugar chitobiose Plumbridge J,2004. 3ChbR and NagC appear to act as repressors for the chbB operon in the absence of the inducing sugar chitobiose Plumbridge J,2004 4ChbR and NagC appear to act as repressors for the chbB operon in the absence of the inducing sugar chitobiose Plumbridge J,2004 5ChbR and NagC appear to act as repressors for the chbB operon in the absence of the inducing sugar chitobiose Plumbridge J,2004 6ChbR and NagC appear to act as repressors for the chbB operon in the absence of the inducing sugar chitobiose Plumbridge J,2004 7NagC and ChbR appear to act as repressors for the chbB operon in the absence of the inducing sugar chitobiose Plumbridge J,2004. |
RNA cis-regulatory element | ![]() |
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Regulation, transcriptional elongation | |
Attenuator type: | Translational |
Strand: | reverse |
Structure type | Energy | LeftPos | RightPos | Sequence (RNA-strand) | |
---|---|---|---|---|---|
terminator | -16.9 | 1819826 | 1819851 | ttttaatcgaTGCCGGTACGGGTAATCGTGCCGGTaagaaaagag | |
terminator | -10.0 | 1821233 | 1821254 | ataaatcaatACCGCAATATTTAAATTGCGGtttttaaggg |
Notes: "The provided "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appear as the reverse complement of the sequence delimited by LeftPos-RigtPos" |
Reference(s) |
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