RegulonDB RegulonDB 10.7: Operon Form
   

creABCD operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: creABCD
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit       
Name: creABC
Gene(s): creA, creB, creC   Genome Browser M3D Gene expression COLOMBOS
Note(s): The function of the creC gene could only be detected in the phoR mutants.
The expression of the gene phoM (creC) is increased under acidic growth conditions in aerobiosis but not in microaerobiosis |CITS:[23274360]|. The increased expression appears to be caused by the transcription factor PhoB |CITS:[23274360]|. This gene is the third in the operon in which it is transcribed, but an effect of these growth conditions and PhoB on the other genes in the operon has not been observed.
Evidence: [ITC] Inferred through co-regulation
Reference(s): [1] Nonaka G., et al., 2006
Promoter
Name: creAp2
+1: 4635243
Sigma Factor: Sigma32 Sigmulon
Distance from start of the gene: 278
Sequence: ttaaacattctctgtaagtgccacttggaataacctgctttcgccgctacattgtcgagcGacaggggctgatccagatga
                         -35                 -10            +1                   
Evidence: [HIPP]
[TIM]
Reference(s): [1] Nonaka G., et al., 2006


Transcription unit          
Name: creABCD
Synonym(s): orf1-orf2-phoM-orf4, phoM
Gene(s): creA, creB, creC, creD   Genome Browser M3D Gene expression COLOMBOS
Note(s): The function of the creC gene could only be detected in the phoR mutants.
J. Oberto in 2010 predicted this binding site for NagC |CITS:[21070640]|, which overlaps with the divergent promoter creAp, but it is unknown whether this protein could regulate the rob gene. In this case it is not known if NagC regulates transcription in both directions.
The expression of the gene phoM (creC) is increased under acidic growth conditions in aerobiosis but not in microaerobiosis |CITS:[23274360]|. The increased expression appears to be caused by the transcription factor PhoB |CITS:[23274360]|. This gene is the third in the operon in which it is transcribed, but an effect of these growth conditions and PhoB on the other genes in the operon has not been observed.
Evidence: [BTEI] Boundaries of transcription experimentally identified
Reference(s): [2] Amemura M., et al., 1986
Promoter
Name: creAp
+1: 4635477
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 44
Sequence: ttagggcttgacggaaataaaagtattgagattttgttcttaatcaatatgttatttaccGtgacgaactaattgctcgtg
                          -35                   -10         +1                   
Evidence: [HIPP]
Reference(s): [2] Amemura M., et al., 1986
Terminator(s)
Type: rho-independent
Sequence: taaggttgaaAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTgacggtggta
Reference(s): [2] Amemura M., et al., 1986
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal NagC1 repressor creAp 4635467 4635489 2.0 taatcaatatGTTATTTACCGTGACGAACTAATtgctcgtgta nd [AIBSCS] [3]
Note(s): 1This binding site was determined from a computational prediction based on different statistical methods Oberto J.,2010 The function of the binding site was assigned according to the distance from the transcription start, with NagC as the transcriptional repressor.1This binding site was determined from a computational prediction based on different statistical methods Oberto J.,2010 The function of the binding site was assigned according to the distance from the transcription start, with NagC as the transcriptional repressor.


Transcription unit          
Name: creD
Gene(s): creD   Genome Browser M3D Gene expression COLOMBOS
Note(s): The expression of the gene creD is increased when colicin (at 30 ng/ml) is added to the medium |CITS: [23421615]|.
Evidence: [BTEI] Boundaries of transcription experimentally identified
Reference(s): [4] Cariss SJ., et al., 2008
Promoter
Name: creDp
+1: 4638160
Distance from start of the gene: 18
Sequence: cttcaccgtcacttcacatagcttcaaattcttcccacatagtcttcgtatcctgctgccAttgcaaaggagaagactatg
Evidence: [TIM]
Reference(s): [4] Cariss SJ., et al., 2008
Terminator(s)
Type: rho-independent
Sequence: taaggttgaaAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTgacggtggta
Reference(s): [2] Amemura M., et al., 1986
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal CreB-Phosphorylated activator creDp 4638099 4638117 -52.5 cctcgcttcgACTTCACCGTCACTTCACAtagcttcaaa nd [AIBSCS], [BPP], [GEA] [4], [5]


RNA cis-regulatory element    
Regulation, transcriptional elongation  
Attenuator type: Translational
Strand: forward
Evidence: [ICA] Inferred by computational analysis
Reference(s): [6] Merino E, et al., 2005
  Structure type Energy LeftPos RightPos Sequence (RNA-strand)
  terminator -19.3 4638137 4638180 ttcttcccacATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGttgaaatccc
Notes: "The provided "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appear as the reverse complement of the sequence delimited by LeftPos-RigtPos"





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