RegulonDB RegulonDB 10.8: Operon Form
   

ribF-ileS-lspA-fkpB-ispH operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: ribF-ileS-lspA-fkpB-ispH
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit       
Name: ribF-ileS-lspA-fkpB-ispH
Synonym(s): ileS-lsp, ileS-lspA-lytB, ribF-ileS-lspA-slpA-lytB
Gene(s): ribF, ileS, lspA, fkpB, ispH   Genome Browser M3D Gene expression COLOMBOS
Evidence: [IEP] Inferred from expression pattern
Reference(s): [1] Kamio Y., et al., 1985
[2] Miller KW., et al., 1987
[3] Miller KW., et al., 1987
Promoter
Name: ribFp
+1: 21383
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 24
Sequence: ttctgatttcggtactcaatcgccggttaaccttgaccgctgtacaaggtatactcggacGattttcactgttttgagcca
                           -35                    -10       +1                   
Evidence: [CV(RPF)]
[CV(RPF/RS-EPT-CBR)]
[CV(RPF/TIM)]
[CV(RS-EPT-CBR)]
[CV(TIM)]
[RPF]
[RS-EPT-CBR]
[TIM]
Reference(s): [1] Kamio Y., et al., 1985
[2] Miller KW., et al., 1987
[4] Salgado H, et al., 2012
Terminator(s)
Type: rho-independent
Sequence: aagttatgcgAAAATGCCGGTCTTGTTACCGGCATTTTttatggagaa
Reference(s): [5] Bouvier J., et al., 1991


Transcription unit       
Name: ileS-lspA-fkpB-ispH
Gene(s): ileS, lspA, fkpB, ispH   Genome Browser M3D Gene expression COLOMBOS
Evidence: [IEP] Inferred from expression pattern
[PM] Polar mutation
Reference(s): [6] Innis MA., et al., 1984
[3] Miller KW., et al., 1987
[7] Regue M., et al., 1984
[8] Tokunaga M., et al., 1985
Promoter
Name: ileSp1
+1: 21833
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 558
Sequence: gtaggtgatgatttccgctttggcgctggtcgtgaaggcgatttcttgttattacagaaaGctggcatggaatacggcttc
                          -35                     -10       +1                   
Evidence: [HIPP]
[TIM]
Reference(s): [2] Miller KW., et al., 1987
[8] Tokunaga M., et al., 1985
Terminator(s)
Type: rho-independent
Sequence: aagttatgcgAAAATGCCGGTCTTGTTACCGGCATTTTttatggagaa
Reference(s): [5] Bouvier J., et al., 1991


Transcription unit       
Name: ileS-lspA-fkpB-ispH
Gene(s): ileS, lspA, fkpB, ispH   Genome Browser M3D Gene expression COLOMBOS
Evidence: [IEP] Inferred from expression pattern
[ITC] Inferred through co-regulation
Reference(s): [6] Innis MA., et al., 1984
[3] Miller KW., et al., 1987
[7] Regue M., et al., 1984
[8] Tokunaga M., et al., 1985
Promoter
Name: ileSp2
+1: 22034
Distance from start of the gene: 357
Sequence: gccatctccgggcgtgtagtccacggtgatgaattagggcgcactataggtttcccgacgGcgaatgtaccgctgcgccgt
Evidence: [TIM]
Reference(s): [2] Miller KW., et al., 1987
Terminator(s)
Type: rho-independent
Sequence: aagttatgcgAAAATGCCGGTCTTGTTACCGGCATTTTttatggagaa
Reference(s): [5] Bouvier J., et al., 1991


Transcription unit       
Name: ileS-lspA-fkpB-ispH
Gene(s): ileS, lspA, fkpB, ispH   Genome Browser M3D Gene expression COLOMBOS
Evidence: [IEP] Inferred from expression pattern
[ITC] Inferred through co-regulation
Reference(s): [6] Innis MA., et al., 1984
[3] Miller KW., et al., 1987
[7] Regue M., et al., 1984
[8] Tokunaga M., et al., 1985
Promoter
Name: ileSp3
+1: 22229
Sigma Factor: Sigma32 Sigmulon
Distance from start of the gene: 162
Sequence: cagctggaagtgcatttgttagatgttgcaatggacctttacggtcgccatatacaagtaGtgctgcgtaaaaaaatacgc
                          -35                 -10           +1                   
Evidence: [AIPP]
[CV(RS-EPT-CBR)]
[CV(TIM)]
[RS-EPT-CBR]
[TIM]
Reference(s): [9] Nonaka G., et al., 2006
[4] Salgado H, et al., 2012
Terminator(s)
Type: rho-independent
Sequence: aagttatgcgAAAATGCCGGTCTTGTTACCGGCATTTTttatggagaa
Reference(s): [5] Bouvier J., et al., 1991


Transcription unit       
Name: lsp-fkpB-ispH
Gene(s): lspA, fkpB, ispH   Genome Browser M3D Gene expression COLOMBOS
Evidence: [IEP] Inferred from expression pattern
Reference(s): [2] Miller KW., et al., 1987
[3] Miller KW., et al., 1987
[8] Tokunaga M., et al., 1985
Promoter
Name: lspAp
+1: 25014
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 193
Sequence: tgggcgatgaattacgatttgtcctgttgacctccggcgctaccgttgcagactataacgAcgcacctgctgatgctcagc
                           -35                   -10        +1                   
Evidence: [CV(TIM)]
[ICWHO]
[IDA]
[TIM]
Reference(s): [1] Kamio Y., et al., 1985
[2] Miller KW., et al., 1987
[3] Miller KW., et al., 1987
[10] Thomason MK., et al., 2015
Terminator(s)
Type: rho-independent
Sequence: aagttatgcgAAAATGCCGGTCTTGTTACCGGCATTTTttatggagaa
Reference(s): [5] Bouvier J., et al., 1991




Reference(s)    

 [1] Kamio Y., Lin CK., Regue M., Wu HC., 1985, Characterization of the ileS-lsp operon in Escherichia coli. Identification of an open reading frame upstream of the ileS gene and potential promoter(s) for the ileS-lsp operon., J Biol Chem 260(9):5616-20

 [2] Miller KW., Bouvier J., Stragier P., Wu HC., 1987, Identification of the genes in the Escherichia coli ileS-lsp operon. Analysis of multiple polycistronic mRNAs made in vivo., J Biol Chem 262(15):7391-7

 [3] Miller KW., Wu HC., 1987, Cotranscription of the Escherichia coli isoleucyl-tRNA synthetase (ileS) and prolipoprotein signal peptidase (lsp) genes. Fine-structure mapping of the lsp internal promoter., J Biol Chem 262(1):389-93

 [4] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [5] Bouvier J., Stragier P., 1991, Nucleotide sequence of the lsp-dapB interval in Escherichia coli., Nucleic Acids Res 19(1):180

 [6] Innis MA., Tokunaga M., Williams ME., Loranger JM., Chang SY., Chang S., Wu HC., 1984, Nucleotide sequence of the Escherichia coli prolipoprotein signal peptidase (lsp) gene., Proc Natl Acad Sci U S A 81(12):3708-12

 [7] Regue M., Remenick J., Tokunaga M., Mackie GA., Wu HC., 1984, Mapping of the lipoprotein signal peptidase gene (lsp)., J Bacteriol 158(2):632-5

 [8] Tokunaga M., Loranger JM., Chang SY., Regue M., Chang S., Wu HC., 1985, Identification of prolipoprotein signal peptidase and genomic organization of the lsp gene in Escherichia coli., J Biol Chem 260(9):5610-5

 [9] Nonaka G., Blankschien M., Herman C., Gross CA., Rhodius VA., 2006, Regulon and promoter analysis of the E. coli heat-shock factor, sigma32, reveals a multifaceted cellular response to heat stress., Genes Dev 20(13):1776-89

 [10] Thomason MK., Bischler T., Eisenbart SK., Forstner KU., Zhang A., Herbig A., Nieselt K., Sharma CM., Storz G., 2015, Global transcriptional start site mapping using differential RNA sequencing reveals novel antisense RNAs in Escherichia coli., J Bacteriol 197(1):18-28


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