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| Operon |  |
|---|---|
| Name: | motAB-cheAW |
| This page displays every known transcription unit of this operon and their known regulation. | |
| Name: | motAB-cheAW |
| Gene(s): | cheW, cheA, motB, motA Genome Browser M3D Gene expression COLOMBOS |
| Note(s): | The CpxR-dependent expression of the motAB-cheAW operon is diminished under the stress condition caused by the introduction of the R1-16 plasmid, which encodes the type IV secretion system (T4S machinery) in the cell Zahrl D,2006 The mRNA produced by the motAB-cheAW operon has been observed mainly in the poles of the cell 31540875. In a study where it was shown that the L-tryptophan eradicated persister cells, it was observed that the exogenous amino acid downregulated the expression of motAB as well as other flagellar genes 31271063. |
| Reference(s): |
[1] Mirel DB., et al., 1992 [2] Silverman M., et al., 1977 |
| Promoter | |
| Name: | motAp |
| +1: | 1977302 |
| Sigma Factor: | Sigma28 Sigmulon |
| Distance from start of the gene: | 163 |
| Sequence: |
accgccatcccgggcagtaaaaagacgtaaactttcccagaatcctgccgatattatcccAcaactgctggatgaacagag -35 -10 +1 |
| Note(s): | The sequence of this promoter has been defined, by computational analyses, from the consensus sequence of the σ28 (σF) promoters TAAAGTTT in the -35 box and GCCGATAA in the -10 box Park K,2001. Ko M,2000. Ide N,1999. Therefore, we assigned a putative transcription start site for this promoter based on the observation that the majority of the σ28 promoters, determined experimentally, present an important -10 motif (5'-CGA-3') at the positions -12 to -10 and a distance of 6 nucleotides between the transcription start site and the -10 box 16855242. Yu HH,2006. Park K,2001 The motAp promoter is an intragenic promoter controlled by FliA, which is inside the flhC gene. The FliA promoter within the flhC gene constrains evolution of the FlhC amino acid sequence. 29476659 Although FlhC has two amino acids (Ala177 and Asp178) overlapping the -10 region, which are highly conserved among γ-proteobacteria, Asp178 is not required for FlhC function 29476659 The Ala-Asp motif is conserved due to selective pressure on the motA promoter, rather than on the amino acids themselves 29476659 motAp, the promoter within the flhC gene, is the most highly conserved of all FliA promoters 29476659 |
| Evidence: | [HIPP] |
| Reference(s): |
[3] Ide N., et al., 1999 [4] Ko M., et al., 2000 [5] Park K., et al., 2001 |
| Terminator(s) | |
| Type: | rho-independent |
| Sequence: | acccgccgcgAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAatcgggagaa |
| Reference(s): |
[6] Feng CQ., et al., 2019 [7] Lesnik EA., et al., 2001 |
| Type | Transcription factor | Function | Promoter | Binding Sites | Growth Conditions | Evidence (Confirmed, Strong, Weak) | Reference(s) | |||
|---|---|---|---|---|---|---|---|---|---|---|
| LeftPos | RightPos | Central Rel-Pos | Sequence | |||||||
| proximal | CpxR-Phosphorylated | repressor | motAp | 1977332 | 1977346 | -37.0 | atcccgggcaGTAAAAAGACGTAAActttcccaga | nd | [BPP], [GEA] | [8], [9] |
| RNA cis-regulatory element | |
|---|
| Regulation, transcriptional elongation | |
| Attenuator type: | Transcriptional |
| Strand: | reverse |
| Structure type | Energy | LeftPos | RightPos | Sequence (RNA-strand) | |
|---|---|---|---|---|---|
| terminator | -16.7 | 1977207 | 1977240 | gcaacattccAGCAGCGGTAACGACGTACCGCTGCTTTTTTTTgccccaatcg | |
| anti-terminator | -5.6 | 1977232 | 1977274 | gagagtacagGCTGTTTAACTGATACGGTGAGGCGCAACATTCCAGCAGCGGtaacgacgta | |
| anti-anti-terminator | -10.6 | 1977252 | 1977305 | ccgatattatCCCACAACTGCTGGATGAACAGAGAGTACAGGCTGTTTAACTGATACGGTGAGgcgcaacatt |
| Notes: "The provided "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appear as the reverse complement of the sequence delimited by LeftPos-RigtPos" |
| Reference(s) |
|
|---|---|
[HIPP] Human inference of promoter position