RegulonDB RegulonDB 10.7: Operon Form

aroG operon and associated TUs in Escherichia coli K-12 genome

Name: aroG
This page displays every known transcription unit of this operon and their known regulation.

Transcription unit          
Name: aroG
Synonym(s): OP00011
Gene(s): aroG   Genome Browser M3D Gene expression COLOMBOS
Note(s): The aroG gene has been shown to be repressed by TyrR in the presence of phenylalanine and tryptophan in vivo, while tyrosine reverses this repression |CITS: [2861143] [4884590]|. However, according to Baseggio et al., the three aromatic amino acids did not show any effect in a footprinting analysis using purified TyrR, although they suggested that the footprinting analysis perhaps lacked a cellular component necessary for the interaction of the aromatic amino acids with TyrR |CITS: [1970563]|. In contrast with the regulatory region of other members of the TyrR regulon that have two or three TyrR boxes, the regulatory region of the aroG gene has a single TyrR box |CITS:[1970563]|.
Evidence: [BTEI] Boundaries of transcription experimentally identified
Reference(s): [1] Baseggio N., et al., 1990
[2] Kikuchi Y., et al., 1997
[3] Nemoz G., et al., 1976
Name: aroGp
+1: 785591
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 42
Sequence: taatccgttcatagtgtaaaaccccgtttacacattctgacggaagatatagattggaagTattgcattcactaagataag
                           -35                    -10       +1                   
Evidence: [CV(RPF/RS-EPT-CBR)]
[TIM] ; Method: Primer Extension
Reference(s): [1] Baseggio N., et al., 1990
[4] Salgado H, et al., 2012
[5] Yamamoto K., et al., 2006
Type: rho-independent
Sequence: tagcaacaaaAAAGCCGACTCACTTGCAGTCGGCTTTctcattttaa
Reference(s): [1] Baseggio N., et al., 1990
[6] Davies WD., et al., 1982
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal CpxR-Phosphorylated activator aroGp 785546 785560 -38.0 cgttcatagtGTAAAACCCCGTTTAcacattctga nd [APIORCISFBSCS], [BPP] [5]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal TyrR-phenylalanine repressor aroGp 785544 785561 -38.0 tccgttcataGTGTAAAACCCCGTTTACacattctgac nd [APIORCISFBSCS], [BPP], [SM] [1]


 [1] Baseggio N., Davies WD., Davidson BE., 1990, Identification of the promoter, operator, and 5' and 3' ends of the mRNA of the Escherichia coli K-12 gene aroG., J Bacteriol 172(5):2547-57

 [2] Kikuchi Y., Tsujimoto K., Kurahashi O., 1997, Mutational analysis of the feedback sites of phenylalanine-sensitive 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase of Escherichia coli., Appl Environ Microbiol 63(2):761-2

 [3] Nemoz G., Robert-Baudouy J., Stoeber F., 1976, Physiological and genetic regulation of the aldohexuronate transport system in Escherichia coli., J Bacteriol 127(2):706-18

 [4] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [5] Yamamoto K., Ishihama A., 2006, Characterization of copper-inducible promoters regulated by CpxA/CpxR in Escherichia coli., Biosci Biotechnol Biochem 70(7):1688-95

 [6] Davies WD., Davidson BE., 1982, The nucleotide sequence of aroG, the gene for 3-deoxy-D-arabinoheptulosonate-7-phosphate synthetase (phe) in Escherichia coli K12., Nucleic Acids Res 10(13):4045-58