RegulonDB RegulonDB 11.2: Operon Form
   

aroG operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: aroG
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: aroG
Synonym(s): OP00011
Gene(s): aroG   Genome Browser M3D Gene expression COLOMBOS
Note(s): The aroG gene has been shown to be repressed by TyrR in the presence of phenylalanine and tryptophan in vivo, while tyrosine reverses this repression Davies WD, Pittard J, Davidson BE,1985. Brown KD,1968. However, according to Baseggio et al., the three aromatic amino acids did not show any effect in a footprinting analysis using purified TyrR, although they suggested that the footprinting analysis perhaps lacked a cellular component necessary for the interaction of the aromatic amino acids with TyrR Baseggio N,1990. In contrast with the regulatory region of other members of the TyrR regulon that have two or three TyrR boxes, the regulatory region of the aroG gene has a single TyrR box Baseggio N,1990
The expression of the gene aroG is enhanced under oxygen limitation Kawaguchi H, Miyagawa H, Nakamura-Tsuruta S, Takaya N, Ogino C, Kondo A,2019.
Evidence: [EXP-IDA-BOUNDARIES-DEFINED] Boundaries of transcription experimentally identified
Reference(s): [1] Baseggio N., et al., 1990
[2] Kikuchi Y., et al., 1997
[3] Nemoz G., et al., 1976
Promoter
Name: aroGp
+1: 785591
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 42
Sequence: taatccgttcatagtgtaaaaccccgtttacacattctgacggaagatatagattggaagTattgcattcactaagataag
                           -35                    -10       +1                   
Evidence: [COMP-AINF]
[COMP-HINF-POSITIONAL-IDENTIFICATION]
[EXP-IDA-RNA-POLYMERASE-FOOTPRINTING]
[EXP-IDA-TRANSCRIPTION-INIT-MAPPING]
[RS-EPT-CBR]
Reference(s): [1] Baseggio N., et al., 1990
[4] Huerta AM., et al., 2003
[5] Salgado H, et al., 2012
[6] Yamamoto K., et al., 2006
Terminator(s)
Type: rho-independent
Sequence: tagcaacaaaAAAGCCGACTCACTTGCAGTCGGCTTTctcattttaa
Reference(s): [1] Baseggio N., et al., 1990
[7] Davies WD., et al., 1982
TF binding sites (TFBSs)
[CpxR,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence Confidence level (C: Confirmed, S: Strong, W: Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal CpxR-phosphorylated activator aroGp 785552 785553 -38.0 agtgtaaaacCccgtttacac nd [COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] nd [6]
[TyrR,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence Confidence level (C: Confirmed, S: Strong, W: Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal TyrR-L-phenylalanine repressor aroGp 785544 785561 -38.0 tccgttcataGTGTAAAACCCCGTTTACacattctgac nd [COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS], [EXP-IMP-SITE-MUTATION] C [1]


Evidence    

 [COMP-AINF] Inferred computationally without human oversight

 [COMP-HINF-POSITIONAL-IDENTIFICATION] Human inference of promoter position

 [EXP-IDA-RNA-POLYMERASE-FOOTPRINTING] RNA polymerase footprinting

 [EXP-IDA-TRANSCRIPTION-INIT-MAPPING] Transcription initiation mapping

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [COMP-HINF-SIMILAR-TO-CONSENSUS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.

 [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] Binding of purified proteins

 [EXP-IMP-SITE-MUTATION] Site mutation


Reference(s)    

 [1] Baseggio N., Davies WD., Davidson BE., 1990, Identification of the promoter, operator, and 5' and 3' ends of the mRNA of the Escherichia coli K-12 gene aroG., J Bacteriol 172(5):2547-57

 [2] Kikuchi Y., Tsujimoto K., Kurahashi O., 1997, Mutational analysis of the feedback sites of phenylalanine-sensitive 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase of Escherichia coli., Appl Environ Microbiol 63(2):761-2

 [3] Nemoz G., Robert-Baudouy J., Stoeber F., 1976, Physiological and genetic regulation of the aldohexuronate transport system in Escherichia coli., J Bacteriol 127(2):706-18

 [4] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [5] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [6] Yamamoto K., Ishihama A., 2006, Characterization of copper-inducible promoters regulated by CpxA/CpxR in Escherichia coli., Biosci Biotechnol Biochem 70(7):1688-95

 [7] Davies WD., Davidson BE., 1982, The nucleotide sequence of aroG, the gene for 3-deoxy-D-arabinoheptulosonate-7-phosphate synthetase (phe) in Escherichia coli K12., Nucleic Acids Res 10(13):4045-58

RegulonDB