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Name: | aroL-yaiA-aroM |
Synonym(s): | OP00013, aroLM |
Gene(s): | aroL, yaiA, aroM Genome Browser M3D Gene expression COLOMBOS |
Note(s): | The expression of the aroL-yiaA-aroM operon seems to be repressed by the interaction of two proteins, TyrR and TrpR, because the effect of TrpR cannot be seen in the absence of TyrR and the effect of TyrR is enhanced in the presence of TrpR. This suggests that a putative physical interaction between TyrR and TrpR or a conformational change in the DNA caused by TyrR facilitates the binding of TrpR. Moreover, TrpR can stabilize TyrR binding Lawley B,1994 In the presence of tyrosine plus ATP, tryptophan, and to a lesser extent phenylalanine, the TyrR protein binds to three TyrR boxes located at positions bp -45, +12, and +31 from the transcriptional start site (TSS) of the aroLp promoter. The -45 and +31 sites are essential to the repression, and the TyrR dimers bound to them seem to interact through the formation of the DNA loop structure that would sequester the RNA polymerase-binding region Lawley B,1994 The +12 site, which is a weak TyrR box, may not be essential for repression Lawley B,1994 A putative IHF site was identified around the +31 TyrR box. This site seems to have a negative effect on the repression mediated by tyrosine Lawley B,1994 |
Evidence: | [LTED] Length of transcript experimentally determined |
Reference(s): | [1] DeFeyter RC., et al., 1986 |
Promoter | |
Name: | aroLp |
+1: | 406280 |
Sigma Factor: | Sigma70 Sigmulon |
Distance from start of the gene: | 125 |
Sequence: |
taaatgtaatttattatttacacttcattcttgaatatttattggtatagtaaggggtgtAttgagattttcactttaagt -35 -10 +1 |
Note(s): | aroLp was validated experimentally in anaerobic conditions Chung D,2013. |
Evidence: | [TIM] |
Reference(s): |
[2] Chung D., et al., 2013 [3] Millar G., et al., 1986 |
Type | Transcription factor | Function | Promoter | Binding Sites | Growth Conditions | Evidence (Confirmed, Strong, Weak) | Reference(s) | |||
---|---|---|---|---|---|---|---|---|---|---|
LeftPos | RightPos | Central Rel-Pos | Sequence | |||||||
remote | TrpR-tryptophan | repressor | aroLp | 406319 | 406336 | 48.5 | ttctttacaaTCGAAATTGTACTAGTTTgatggtatga | nd | [APIORCISFBSCS], [BPP], [CV(GEA)], [CV(GEA)], [CV(GEA/SM)], [CV(SM)], [GEA], [SM] | [4], [5], [6], [7] |
remote | TrpR-tryptophan | repressor | aroLp | 406327 | 406344 | 56.5 | aatcgaaattGTACTAGTTTGATGGTATgatcgctatt | nd | [APIORCISFBSCS], [BPP], [CV(GEA)], [CV(GEA)], [CV(GEA/SM)], [CV(SM)], [GEA], [SM] | [4], [5], [6], [7] |
Type | Transcription factor | Function | Promoter | Binding Sites | Growth Conditions | Evidence (Confirmed, Strong, Weak) | Reference(s) | |||
---|---|---|---|---|---|---|---|---|---|---|
LeftPos | RightPos | Central Rel-Pos | Sequence | |||||||
proximal | TyrR-tyrosine | repressor | aroLp | 406226 | 406243 | -45.0 | tggctaaatgTAATTTATTATTTACACTtcattcttga | nd | [BPP], [CV(SM)], [GEA], [SM] | [1], [4], [5] |
proximal | TyrR-tyrosine | repressor | aroLp | 406282 | 406299 | 12.0 | aggggtgtatTGAGATTTTCACTTTAAGtggaattttt | nd | [BPP], [CV(SM)], [GEA], [SM] | [1], [4], [5] |
remote | TyrR-tyrosine | repressor | aroLp | 406301 | 406318 | 31.0 | cactttaagtGGAATTTTTTCTTTACAAtcgaaattgt | nd | [BPP], [CV(SM)], [GEA], [SM] | [1], [4], [5] |
Name: | aroL-yaiA-aroM |
Gene(s): | aroL, yaiA, aroM Genome Browser M3D Gene expression COLOMBOS |
Evidence: | [LTED] Length of transcript experimentally determined |
Reference(s): | [1] DeFeyter RC., et al., 1986 |
Promoter | |
Name: | aroLp2 |
+1: | 406355 |
Distance from start of the gene: | 50 |
Sequence: |
ttaagtggaattttttctttacaatcgaaattgtactagtttgatggtatgatcgctattCtcatgacaccggctttcgcc |
Note(s): | aroLp2 was validated experimentally in anaerobic conditions Chung D,2013. |
Evidence: | [TIM] |
Reference(s): | [2] Chung D., et al., 2013 |
Name: | aroM |
Gene(s): | aroM Genome Browser M3D Gene expression COLOMBOS |
Evidence: | [IEP] Inferred from expression pattern |
Reference(s): | [8] Li Z., et al., 2019 |
Promoter | |
Name: | aroMp |
+1: | 407400 |
Sigma Factor: | Sigma70 Sigmulon |
Distance from start of the gene: | 28 |
Sequence: |
tgtcatttgtagcacaagtaagtgtcagcagtggtgcttcacacttgcccggtaattaacGacgaaagaaaagtaaggtgg -35 -10 +1 |
Evidence: |
[RE] [TIM] |
Reference(s): | [8] Li Z., et al., 2019 |
Name: | yaiA-aroM |
Gene(s): | yaiA, aroM Genome Browser M3D Gene expression COLOMBOS |
Promoter | |
Name: | yaiAp |
+1: | Unknown |
Note(s): | Zaslaver et al. demonstrated in 2006, by means of a library of fluorescent transcription fusions, that this promoter can be transcribed in vitro Zaslaver A,2006. Based on this, a putative promoter was suggested, but the +1 site of the transcription initiation has not been determined, although there exists promoter activity. |
Evidence: | [IEP] |
Reference(s): | [9] Zaslaver A., et al., 2006 |
Reference(s) |
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