RegulonDB RegulonDB 10.10: Operon Form

fhuACDB operon and associated TUs in Escherichia coli K-12 genome

Name: fhuACDB
This page displays every known transcription unit of this operon and their known regulation.

Transcription unit          
Name: fhuACDB
Synonym(s): OP00027, fhuA
Gene(s): fhuA, fhuC, fhuD, fhuB   Genome Browser M3D Gene expression COLOMBOS
Reference(s): [1] Burkhardt R., et al., 1987
[2] Fecker L., et al., 1983
[3] Koster W., et al., 1986
Name: fhuAp
+1: 167442
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 42
Sequence: tgcgtaccgcttgcgaacccgccagcgtttcgaatattatcttatctttataataatcatTctcgtttacgttatcattca
                            -35                  -10        +1                   
Evidence: [AIPP]
Reference(s): [4] Huerta AM., et al., 2003
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal Fur-Fe2+ repressor fhuAp 167430 167448 -3.0 atcttatcttTATAATAATCATTCTCGTTtacgttatca nd [GEA], [APIORCISFBSCS], [BPP] [5], [6], [7], [8]

RNA cis-regulatory element    
Regulation, transcriptional elongation  
Attenuator type: Translational
Strand: forward
Evidence: [ICA] Inferred by computational analysis
Reference(s): [9] Merino E, et al., 2005
  Structure type Energy LeftPos RightPos Sequence (RNA-strand)
  terminator -16.5 169739 169758 tctcttttggGGCACGGATTTCCGTGCCCatttcacaag
Notes: "The provided "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appear as the reverse complement of the sequence delimited by LeftPos-RigtPos"


 [1] Burkhardt R., Braun V., 1987, Nucleotide sequence of the fhuC and fhuD genes involved in iron (III) hydroxamate transport: domains in FhuC homologous to ATP-binding proteins., Mol Gen Genet 209(1):49-55

 [2] Fecker L., Braun V., 1983, Cloning and expression of the fhu genes involved in iron(III)-hydroxamate uptake by Escherichia coli., J Bacteriol 156(3):1301-14

 [3] Koster W., Braun V., 1986, Iron hydroxamate transport of Escherichia coli: nucleotide sequence of the fhuB gene and identification of the protein., Mol Gen Genet 204(3):435-42

 [4] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [5] Chen Z., Lewis KA., Shultzaberger RK., Lyakhov IG., Zheng M., Doan B., Storz G., Schneider TD., 2007, Discovery of Fur binding site clusters in Escherichia coli by information theory models., Nucleic Acids Res 35(20):6762-77

 [6] Coulton JW., Mason P., Cameron DR., Carmel G., Jean R., Rode HN., 1986, Protein fusions of beta-galactosidase to the ferrichrome-iron receptor of Escherichia coli K-12., J Bacteriol 165(1):181-92

 [7] Gerken H., Vuong P., Soparkar K., Misra R., 2020, Roles of the EnvZ/OmpR Two-Component System and Porins in Iron Acquisition in Escherichia coli., mBio 11(3)

 [8] Hantke K., 1981, Regulation of ferric iron transport in Escherichia coli K12: isolation of a constitutive mutant., Mol Gen Genet 182(2):288-92

 [9] Merino E, Yanofsky C., 2005, Transcription attenuation: a highly conserved regulatory strategy used by bacteria., Trends Genet. 2005 May;21(5):260-4.