RegulonDB RegulonDB 10.6.3: Operon Form
   

glpD operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: glpD
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: glpD
Synonym(s): OP00038
Gene(s): glpD   Genome Browser M3D Gene expression COLOMBOS
Reference(s): [1] Iuchi S., et al., 1990
Promoter
Name: glpDp
+1: 3561971
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 42
Sequence: tcactctaaaatgttttttcaatgttacctaaagcgcgattctttgctaatatgttcgatAacgaacatttatgagcttta
                         -35                    -10         +1                   
Note(s): In a gene expression analysis, RpoS positively regulated the glpD gene in the stationary phase. Its effect could be indirect Becker-Hapak M,1995
Evidence: [TIM] ; Method: Primer Extension
[TIM]
Reference(s): [2] Yang B., et al., 1996
[3] Ye SZ., et al., 1988
TF binding sites (TFBSs)
[ArcA,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd ArcA-Phosphorylated repressor glpDp nd nd nd nd nd [GEA] [1]
[CRP,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal CRP-cAMP activator glpDp 3561897 3561918 -63.5 ataaacgccaTAATGTTATACATATCACTCTAaaatgttttt nd [BPP], [GEA], [HIBSCS] [2], [3], [4]
[GlpR,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal GlpR repressor glpDp 3561961 3561980 -0.5 tctttgctaaTATGTTCGATAACGAACATTtatgagcttt nd [BPP], [GEA], [HIBSCS] [2], [3], [5]
proximal GlpR repressor glpDp 3561980 3561999 19.5 taacgaacatTTATGAGCTTTAACGAAAGTgaatgagggc nd [BPP], [GEA], [HIBSCS] [2], [3], [5]
remote GlpR repressor glpDp 3562375 3562394 414.5 cgggatcaacTGGTTTGCGTTTTGGCGCAAattcagtgtt nd [BPP], [GEA], [HIBSCS], [SM] [2]
remote GlpR4 repressor glpDp 3562416 3562435 455.5 aaaccggaaaTTAAGCGCGGATTCGAATATtctgactgtt nd [BPP], [GEA], [HIBSCS], [SM] [2]
Note(s): 4Although the internal operators required sites at +1 and +20 for repression in vivo, these sites were not required to demonstrate the binding of GlpR to sites +415.5 and +456.5.5Although the internal operators required sites at +1 and +20 for repression in vivo, these sites were not required to demonstrate the binding of GlpR to sites +415.5 and +456.5.


Evidence    

 [TIM] Transcription initiation mapping

 [GEA] Gene expression analysis

 [BPP] Binding of purified proteins

 [HIBSCS] Human inference based on similarity to consensus sequences

 [SM] Site mutation


Reference(s)    

 [1] Iuchi S., Cole ST., Lin EC., 1990, Multiple regulatory elements for the glpA operon encoding anaerobic glycerol-3-phosphate dehydrogenase and the glpD operon encoding aerobic glycerol-3-phosphate dehydrogenase in Escherichia coli: further characterization of respiratory control., J Bacteriol 172(1):179-84

 [2] Yang B., Larson TJ., 1996, Action at a distance for negative control of transcription of the glpD gene encoding sn-glycerol 3-phosphate dehydrogenase of Escherichia coli K-12., J Bacteriol 178(24):7090-8

 [3] Ye SZ., Larson TJ., 1988, Structures of the promoter and operator of the glpD gene encoding aerobic sn-glycerol-3-phosphate dehydrogenase of Escherichia coli K-12., J Bacteriol 170(9):4209-15

 [4] Weissenborn DL., Wittekindt N., Larson TJ., 1992, Structure and regulation of the glpFK operon encoding glycerol diffusion facilitator and glycerol kinase of Escherichia coli K-12., J Biol Chem 267(9):6122-31

 [5] Zhao N., Oh W., Trybul D., Thrasher KS., Kingsbury TJ., Larson TJ., 1994, Characterization of the interaction of the glp repressor of Escherichia coli K-12 with single and tandem glp operator variants., J Bacteriol 176(8):2393-7

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